Abstract
In the course of screening for immunomodulators, we found a significant blastogenic activity specific for splenic B cells in the extracts of safflower (Carthamus tinctorius L.). Active fractions termed SF1 and SF2 were purified from dried petals of safflower by boiling water extraction, ethanol precipitation and Sepharose CL-2B column chromatography. The elution profiles of the gel filtration indicated that the molecular weight of SF1 and SF2 was estimated to be more than 100 kD. Major components of SF1 and SF2 seem to be polysaccharides, and structural analysis of alditol acetate derivatives by GC-MS revealed some differences between SF1 and SF2 in the sugar component. Biological activities of SF1 and SF2 on B cells and macrophages were examined in comparison with lipopolysaccharides (LPS). SF1 and SF2 induced both the proliferation and the IgM production of B cells to the equivalent level as those induced by LPS. In macrophages, SF1 and SF2 effectively stimulated the production of NO. However, SF1 stimulated the production of IL-1, IL-6, and TNF as much as LPS, while SF2 induced them only weakly or not at all. Thus, these results suggest that SF1 and SF2 activate B cells and macrophages in different mechanisms.
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References
Brander WT, Clarke DA and Stock CC (1958) Stimulation of Host Defence against Experimental Cancer I. Zymosan and Sarcoma 180 in mice. Cancer Res 18: 347–351.
Brander WT and Clarke DA (1959) Stimulation of Host Defence against Experimental Cancer II. Temporal and Reversal Studies of the Zymosan Effect. Cancer Res 19: 673–678.
Chihara G, Maeda Y, Hamuro J, Sasaki T and Fukuoka F (1969) Inhibition of Mouse Sarcoma 180 by Polysaccharides from Lentinus edodes (Berk.) Sing. Nature 222: 687–688.
Chihara G, Hamoro J, Maeda YY, Arai Y and Fukuoka F (1970) Fractionation and Purification of the Polysaccharides with Marked Antitumor Activity, Especally Lentinan, from Lentinus edodes (Berk.) Sing. (an Edible Mushroom). Cancer Res 30: 2776–2781.
Cohen L, Haziot A, Shen DR, Lin X, Sia C, Harper R, Silver J and Goyert SM (1995) CD14-Independent Responses to LPS Require a Serum Factor That Is Absent from Neonates. J Immunol 155: 5337–5342.
Haziot A, Ferreo E, Köntgen F, Hijima N, Yamamoto S, Silver J, Stewart CL and Goyert SM (1996) Resistance to Endotokin Shock and Reduced Dissemination of Gram-Negative Bacteria in CD14-Deficient Mice. Immunity 4: 407–414.
Hofstad T, Skaug N and Sveen K (1993) Stimulation of B Lymphocytes by Lipopolusaccharides from Anaerobic Bacteria. Clin Infect Dis 16: 200–202.
Itoh W, Sugawara I, Kimura S, Tabata K, Hirata A, Kojima T, Mori S and Shimada K (1990) Immunopharmacological study of schizophyllan (SPG). I. — Its action as a mitogen and anti-HIV agent. Int J Immunopharmacol 12: 225–233.
Kalechman Y, Albeck M, Oron M, Sobelman D, Gurwith M, Seghal S and Sredni B (1990) Radioprotective effects of the immunomodulator AS101. J Immunol 145: 1512–1517.
Kalechman Y, Shani A, Dovrat S, Whisnant JK, Mettinger K, Albeck M and Sredni B (1996) The Antitumoral Effect of the Immunomodulator AS101 and Paclitaxel (Taxol) in Murine Model of Lung Adenocarcinoma. J Immunol 156: 1101–1109.
Kato M, Hirose K, Hakozaki M, Ohno M, Saito Y, Izutani R, Noguchi J, Hori Y, Okumoto S and Kuroda D (1995) Induction of gene expression for immunomodulating cytokines in peripheral blood mononuclear cells in response to orally administered PSK, an immunomodulating protein-bound polysaccharide. Cancer Immunol Immunother 40: 152–156.
Kawaguchi T, Suematsu M, Koizumi HM, Mitsui H, Suzuki S, Matsuno T, Ogawa H and Nomoto K (1983) Activation of Macrophage Function by Intraperitoneal Administration of the Streptococcal Antitumor Agent OK-432. Immunopharmacol 6: 177–189.
Kurakata Y, Sakagami H, Takeda M, Konno K, Kitajima K, Ichikawa S, Hara N and Sato T (1989) Mitogenic activity of pine cone extracts against cultured splenocytes from normal and tumorbearing animals. Anticancer Res 9: 961–966.
Lim BO, Yamada K, Yoshimura K, Watanabe T, Hung P, Taniguchi S and Sugano M (1995) Free Bile Acids Inhibit IgE Production by Mouse Spleen Lymphocytes Stimulated by Lipopolysaccharide and Interleukins. Biosci Biotech Biochem 59: 624–627.
Matsuda T, Hirano T and Kishimoto T (1988) Establishment of an interleukin 6 (IL-6)/B cell stimulatory factor 2-dependent cell line and preparation of anti-IL-6 monoclonal antibodies. Eur J Immunol 18: 951–956.
Mizuno T, Kinoshita T, Zhuang C, Ito H and Mayuzumi Y (1995) Antitumor-active heteroglycans from Niohshimeji mushroom, Tricholoma giganteum. Biosci Biotech Biochem 59: 568–571.
Nemoto J, Ohno N, Saito K, Adachi Y and Yadomae T (1994) Analysis of Cytokine mRNAs Induced by the Administration of a Highly Branched (1→3)-beta-D-blucan, OL-2. Biol Pharm Bull 17: 948–954.
Nick JA, Avdi NJ, Gerwins P, Johnson GL and Worthen GS (1996) Activation of a p38 Mitogen-Activated Protein Kinase in Human Neutrophils by Lipopolysaccharide. J Immunol 156: 4867–4875.
Sakagami H, Sugaya K, Utsumi A, Fujinaga S, Sato T and Takeda M (1993) Stimulation by PSK of Interleukin-1 Production by Human Peripheral Blood Mononuclear Cells. Anticancer Res 13: 671–676.
Stimpel M, Proksch A, Wagner H and Lohmann-Matthes ML (1984) Macrophage Activation and Induction of Macrophage Cytotoxicity by Purified Polysaccharide Fractions from the plant Echinacea purpurea. Infect Immun 46: 845–849.
Takasuka N, Tokunaga T and Akagawa K (1991) Preexposure of macrophages to low doses of lipopolysaccharide inhibits the expression of tumor necrosis factor-α mRNA but not of IL-1β mRNA. J Immunol 146: 3824–3830.
Wang G, Zhang J, Mizuno T, Zhuang C, Ito H, Mayuzumi H, Okamoto H and Li J (1993) Antitumor Active Polysaccharides from the Chinese Mushroom Songshan Lingzhi, the Fruiting Body of Ganoderma tsugae. Biosci Biotech Biochem 57: 894–900.
Ziegler-Heitbrock HWL and Ulevitch RJ (1993) CD14: Cell surface receptor and differentiation marker. Immunol Today 14: 121–125.
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Wakabayashi, T., Hirokawa, S., Yamauchi, N. et al. Immunomodulating activities of polysaccharide fractions from dried safflower petals. Cytotechnology 25, 205–211 (1997). https://doi.org/10.1023/A:1007947329496
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DOI: https://doi.org/10.1023/A:1007947329496