Abstract
The effect of Ca2+/calmodulin (CaM) on the specific binding of [125I]omega-conotoxin GVIA (125I-ω-CTX) to crude membranes from chick brain was investigated. When we examined the effects of the activation of various endogenous protein kinases on specific [125I]ω-CTX binding to crude membranes, we observed that Ca2+/CaM had an inhibitory effect regardless of whether or not the standard medium contained ATP (0.5 mM). Ca2+/CaM also had an inhibitory effect in a simple binding-assay medium containing HEPES-HCl buffer, BSA, Ca2+ and CaM, and this effect was dependent on the concentration of Ca2+. The effect of Ca2+/CaM was attenuated by the CaM antagonists W-7 and CaM-kinase II fragment (290–309). An experiment with modified ELISA using purified anti ω-CTX antibody indicated that Ca2+/CaM did not affect the direct binding of [125I]ω-CTX and CaM. These results suggest that Ca2+/CaM either directly or indirectly affects specific [125I]ω-CTX binding sites, probably N-type Ca2+ channels in crude membranes from chick whole brain.
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Ichida, S., Abe, J., Yu-an, Z. et al. Calcium/Calmodulin Inhibits the Binding of Specific [125I]Omega-Conotoxin GVIA to Chick Brain Membranes. Neurochem Res 25, 335–340 (2000). https://doi.org/10.1023/A:1007532818918
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DOI: https://doi.org/10.1023/A:1007532818918