Abstract
N-glycans of neuropsin (serine protease in the murine hippocampus) expressed in Trichoplusia ni cells were released from the glycopeptides by digestion with glycoamidase A (from sweet almond), and the reducing ends of the oligosaccharides were reductively aminated with 2-aminopyridine. The derivatized N-glycans were separated and structurally identified by a two dimensional high-performance liquid chromatography (HPLC) mapping technique on two kinds of HPLC columns. Fourteen different major N-glycan structures were identified, of which 6 were high-mannose type (9.1%), and the remaining 8 were paucimannosidic type. The presence of insect specific N-glycan structures containing both α1,3- and α1,6- di-fucosylated innermost N-acetylglucosamine residue (23.3%), as below, was also confirmed by 600 MHz 1H-NMR spectroscopy.
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Takahashi, N., Tsukamoto, Y., Shiosaka, S. et al. N-glycan structures of murine hippocampus serine protease, neuropsin, produced in Trichoplusia ni cells. Glycoconj J 16, 405–414 (1999). https://doi.org/10.1023/A:1007082612019
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DOI: https://doi.org/10.1023/A:1007082612019