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The analysis of the poly(ADPR)polymerase mode of action in rat testis nuclear fractions defines a specific poly(ADP-ribosyl)ation system associated with the nuclear matrix

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Abstract

The poly(ADP-ribosyl)ation system, associated with different nuclear fractions of rat testis, has been analyzed for both pADPR and pADPR acceptor proteins. The DNase I sensitive and resistant chromatin contain 35% and 40%, respectively, of the total pADPR synthesized in intact nuclei incubated with [32P]NAD. Moreover, the residual 25% were estimated to be associated with the nuclear matrix.Three different classes of pADPR are present in the nuclei. The longest and branched ADPribose polymers modify proteins present in the DNase I resistant (2 M NaCl extractable) chromatin and in the nuclear matrix, whereas polymers of > 20 residues interact with the components of the DNase I sensitive chromatin and oligomers of 6 ADPribose residues are bound specifically to the acid-soluble chromosomal proteins, present in isolated nuclear matrix. The main pADPR acceptor protein in all the nuclear fractions is represented by the PARP itself (auto-modification reaction). The hetero-modification reaction occurs mostly on histone H1 and core histones, that have been found associated to DNase I sensitive and resistant chromatin, respectively. Moreover, an oligo(ADP-ribosyl)ation occurs on core histones tightly-bound to the matrix associated regions (MARs) of chromatin loops.

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Authors and Affiliations

  1. Department of Organic and Biological Chemistry, University Federico II of Naples, Via Mezzocannone 16, 80134, Napoli, Italy

    Piera Quesada, Filomena Tramontano, M. Rosaria Faraone-Mennella & Benedetta Farina

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  1. Piera Quesada
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  2. Filomena Tramontano
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  3. M. Rosaria Faraone-Mennella
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  4. Benedetta Farina
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Quesada, P., Tramontano, F., Faraone-Mennella, M.R. et al. The analysis of the poly(ADPR)polymerase mode of action in rat testis nuclear fractions defines a specific poly(ADP-ribosyl)ation system associated with the nuclear matrix. Mol Cell Biochem 205, 91–99 (2000). https://doi.org/10.1023/A:1007005715848

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