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Sterol carrier protein-2 suppresses microsomal acyl-CoA hydrolysis

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Abstract

Although sterol carrier protein 2 (SCP-2) has long been regarded primarily as a sterol transfer protein, its actual physiological function is not known. The recent discovery that SCP-2 binds long chain fatty acyl-CoAs (LCFA-CoAs) with high affinity suggests additional roles for SCP-2 in cellular utilization of LCFA-CoAs for synthesis of glycerides and cholesterol esters. Concomitant to these anabolic pathways, LCFA-CoAs are also degraded by cellular hydrolases. The purpose of the work presented herein was to determine if SCP-2 altered the aqueous pool of LCFA-CoA by (i) extracting LCFA-CoA from microsomal membranes, and (ii) protecting LCFA-CoA from microsomal hydrolase activity. The data demonstrated for the first time that SCP-2 increases the aqueous pool of oleoyl-CoA by increasing the aqueous/membrane distribution oleoyl-CoA by 2.4-fold. In addition, SCP-2 inhibited the hydrolysis of oleoyl-CoA by microsomal acyl-CoA hydrolase 1.6-2.4 fold, depending on the concentration of oleoyl-CoA. By simultaneously extracting LCFA-CoA from membranes and inhibiting LCFA-CoA degradation SCP-2 may potentiate LCFA-CoA transacylation and modulate the role of LCFA-CoAs as intracellular signaling molecules.

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Authors and Affiliations

  1. Department of Physiology and Pharmacology, Texas A&M University, TVMC, College Station, TX, 77843-4466, USA

    Christopher A. Jolly, Hsu Chao & Friedhelm Schroeder

  2. Department of Pathobiology, Texas A&M University, TVMC, College Station, TX, 77843-4466, USA

    Ann B. Kier

  3. Cardiovascular Department, DuPont Merck Pharmaceutical Company, Experimental Station 400-3231, Wilmington, DE, 19898-0400, USA

    Jeffrey T. Billheimer

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  1. Christopher A. Jolly
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  2. Hsu Chao
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  4. Jeffrey T. Billheimer
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  5. Friedhelm Schroeder
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Jolly, C.A., Chao, H., Kier, A.B. et al. Sterol carrier protein-2 suppresses microsomal acyl-CoA hydrolysis. Mol Cell Biochem 205, 83–90 (2000). https://doi.org/10.1023/A:1007001614939

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