Abstract
Glutathione plays an important role in various cellular functions including cell growth and differentiation. In the present study, cell differentiation was induced by butyrate in human colon cell line HT-29 and cellular thiol status was assessed. It was observed that butyrate-induced differentiation was associated with decrease in cellular GSH level and this was prominent at early stages of differentiation. Buthionine sulfoximine (BSO), a specific cellular GSH depleting agent, did not induce differentiation in cells but potentiated the differentiation induced by butyrate. Both BSO and butyrate individually and together inhibited cell growth. These studies suggest that cellular GSH level is modulated in butyrate-induced differentiation and decrease of GSH at the initial stage might facilitate cellular differentiation.
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References
Miester A: Selective modification of Glutathione metabolism. Science 220: 470–477, 1983
Deneke SM, Fanburg BL: Regulation of cellular glutathione. Am J Physiol 257: L163–L173, 1988
Arrick BA, Nathan CF: Glutathione metabolism as a determinant of therapeutic efficacy: A review. Cancer Res 44: 4224–4232, 1984
Terradez P, Asensi M, Lasso delareza MC, Puertes IR, Vina J Estrela JM: Depletion of tumor glutathione in vivo by buthionine sulfoximine: modulation by the rate of cellular proliferation and inhibition of cancer growth. Biochem J 292: 477–483, 1993
Shaw JP, Chou IN: Elevation of intracellular glutathione content associated with mitogenic stimulation of quiescent fibroblasts. J Cell Physiol 129: 193–198, 1986
Suthanthiran M, Anderson ME, Sharma VK, Meister A: Glutathione regulates activation-dependent DNA synthesis in highly purified normal human T lymphocytes stimulated via the CD2 and CD3 antigens. Proc Natl Acad Sci USA 87: 3343–3347, 1990
Cummings JH: Short chain fatty acids in the human colon. Gut 22: 763–779, 1981
Cummings JH, Englyst HN: Fermentation in the human large intestine and the available substrates. Am J Clin Nutr 45: 1243–1255, 1987
Petit JM, Chauftert B, Dimanche-Boitrel MT, Genne P, Duchamp O, Martin F: mdr 1 gene-expression and villin synthesis in a colon cancer cell line differentiated by sodium butyrate. Anticancer Res 13: 487–490, 1993
Leder A, Leder P: Butyric acid, a potent inducer of erythroid differentiation in cultured erythroleukemia cells. Cell 5: 319–322, 1975
Young GP, Gibson P: Contrasting effects of butyrate on proliferation and differentiation of normal and neoplastic cells. In: AF Roche (ed). Short chain fatty acids metabolism and clinical importance. Report of the tenth Ross conference on medical research. Columbus, Ohio: Ross Laboratories, 1991 pp 50–55
Chen ZX, Breitman TR: Tributyrin: A prodrug of butyric acid for potential clinical application in differentiation therapy. Cancer Res 54: 3494–3499, 1994
Breitman TR, He R: Combinations of retinoic acid with either sodium butyrate, dimethyl sulfoxide or hexamethylene bisacetamide synergistically induce differentiation of the human myeloid leukemia cell line HL-60. Cancer Res 50: 6268–6273, 1990
Faris WW, Reed DJ: High Performance liquid chromatography of thiols and disulfides: dinitrophenol derivatives. Meth Enzymol 143: 101–109, 1987
Thambidurai D, Bachhwat BK: Purification and properties of Brain Alkaline phosphatase. J Neurochem 29: 503–512, 1979
Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ: Protein measurement with the Folin-phenol reagent. J Biol Chem 193: 265–275, 1951
Esposito F, Agosti V, Morrone G, Morra F, Cuomo C, Russo T, Vienuta S, Cimino F: Inhibition of the differentiation of human myeloid cell lines by redox changes induced through glutathione depletion. Biochem J 301: 649–653, 1994
Mallery SR, Lautry LE, Lauman HB, Stephens RE, Brierly GP: Modulation of human microvascular endothelial cell bioenergetic status and glutathione levels during proliferative and differentiated growth. J cell Biochem 53: 360–372, 1993
Cornell JS, Meister A: Glutathione and γ-glutamyl cycle enzymes in crypt and villus tip cells of rat jejunal mucosa. Proc Natl Acad Sci USA 73: 420–422, 1976
Staal FJT, Roederer M, Herzenberg LA: Intracellular thiols regulate activation of nuclear factor kB and transcription of human immuno-deficiency virus. Proc Natl Acad Sci USA 87: 9943–9947, 1990
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Benard, O., Balasubramanian, K. Modulation of glutathione level during butyrate-induced differentiation in human colon derived HT-29 cells. Mol Cell Biochem 170, 109–114 (1997). https://doi.org/10.1023/A:1006892929652
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DOI: https://doi.org/10.1023/A:1006892929652