Abstract
Following the first official report of a clinically severe outbreak of bovine viral diarrhoea disease occurring in a farm in northern Italy, which had originated from the use of a live vaccine contaminated with a strain of BVD genotype II virus, a retrospective study on the prevalence of BVDV genotypes in Italy became highly relevant. For this purpose, the genotype of 78 BVDV-positive specimens, obtained in 1998–1999 from dairy cattle in an area near to where the outbreak occurred, was characterized by PCR technology. Two sets of primers, spanning the 5′ UTR of BVDV genome, were used sequentially in a first round of RT-PCR, performed on viral RNA extracted directly from 15 clinical samples and 63 BVDV-infected cell-culture fluids; a second PCR assay followed to selectively amplify only BVDV genotype II. All the viruses under study were characterized as BVDV genotype I. As well as contributing to a better understanding of the prevalence of BVDV genotypes in the field, the results of the present study illustrate the possibility that novel BVDV strains can emerge in susceptible animals through the use of contaminated immunobiological products for bovine use.
Similar content being viewed by others
REFERENCES
Baker, J., 1987. Bovine viral diarrhoea virus: a review. Journal of the American Veterinary Medical Association, 190, 1449-1458
Collett, M.S., Larson, R., Gold, C., Strick, D., Anderson, D.K. and Purchio, A.F., 1988.Molecular cloning and nucleotide sequence of the pestivirus bovine viral diarrhea virus.Virology, 165, 191-199
Cordioli, P., Berlinzani, A. and Brocchi, E., 1993. Detection of cattle persistently infected with pestivirus by a sandwich ELISA with anti-p80 monoclonal antibodies. In: S. Edwards (ed.), Proceedings of the Second Symposium on Pestiviruses, 1992, (European Society for Veterinary Virology, Lyon: fondation Marcel Merieux), 203-204
El-Kholy, A.A., Bolin, S.R., Ridpath, J.F., Arab, R.M.H., Abou-Zeid, A.A., Hamman, H.M. and Platt, K.B., 1998. Use of polymerase chain reaction to simultaneously detect and type bovine viral diarrhoea viruses isolated from clinical specimens. Revue Scientifique et Technique de l'Office International des Epizooties, 17, 733-742
Falcone, E., Tollis, M. and Conti, G., 1999. Bovine viral diarrhea disease associated with a contaminated vaccine. Virology, 18, 387-388
Harpin, S., Elahi, S.M., Cornaglia, E., Yolken, R.H. and Elazhary, Y., 1995. The 5′-untranslated region sequence of a potential new genotype of bovine viral diarrhea virus. Archives of Virology, 140, 1285-1290
Letellier, C., Kerkhofs, P., Wellemans, G. and Vanopdenbosch, E., 1999. Detection and genotyping of bovine viral diarrhea virus by reverse transcription–polymerase chain amplification of the 5′ untranslated region.Veterinary Microbiology, 64, 155-167
Pellerin, C., Van Den Hurk, J., Lecomte, J. and Tijssen, P., 1994. Identification of a new group of bovine viral diarrhea virus strains associated with severe outbreaks and high mortalities. Virology, 203, 260-268
Ridpath, J.F., Bolin, S.R. and Dubovi, E.J., 1994. Segregation of bovine diarrhea virus into genotypes. Virology, 205, 66-74
Sambrook, J., Fritsch, E.F. and Maniatis, T., 1989. Molecular Cloning: A LaboratoryManual, (Cold Spring Harbor Laboratory Press, Plainview, NY), 6.3-6.19
Topliff, C.L. and Kelling, C.L., 1998.Virulence markers in the 5′ untranslated region of genotype 2 bovine viral diarrhea virus isolates.Virology, 250, 164-172
Vilcek, S., Drew, T.W., McGoldrick, A. and Paton, D.J., 1999. Genetic typing of bovine pestiviruses from England and Wales.Veterinary Microbiology, 69, 227-237
Wolfmeyer, A., Wolf, G., Beer, M., Strube, W., Hehnen, H.R., Schmeer, N. and Kaaden, O.R., 1997. Genomic (5′ UTR) and serological di¡erences among German BVDV field isolates. Archives of Virology, 142, 2049-2057
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Falcone, E., Cordioli, P., Sala, G. et al. Genotyping of Bovine Viral Diarrhoea Viruses Isolated from Cattle in Northern Italy. Vet Res Commun 25, 161–167 (2001). https://doi.org/10.1023/A:1006465120869
Issue Date:
DOI: https://doi.org/10.1023/A:1006465120869