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Genomic organization of hsp90 gene family in Arabidopsis

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Abstract

We have isolated six members of the hsp90 gene family from Arabidopsis thaliana. Three genes designated hsp81.2, 81.3 and 81.4 are clustered within a 15 kb genomic region while two of these are 1.5 kb apart in a head-to-head orientation. The deduced amino acid sequence shows that the members can be divided into two types. The hsp81.1, 81.2, 81.3 and 81.4 genes comprise the cytosolic hsp90 type having few introns. However, the hsp88.1 and 89.1 genes comprising the organelle type are composed of 18 or 19 introns. Sequence comparison showed there is high homology among the cytosolic members while there is less homology among the organelle members. The expression of the hsp90 genes and mRNA accumulation in plants and calli is very low at control temperatures and is strongly induced by heat-shock. Arsenite stress strongly stimulates the expression of this gene family.

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Authors and Affiliations

  1. Laboratory of Molecular Biology, Department of Agricultural Biology and Biotechnology, Agricultural University of Athens, Iera Odos 75, 118 55, Athens, Greece

    Dimitra Milioni & Polydefkis Hatzopoulos

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  1. Dimitra Milioni
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  2. Polydefkis Hatzopoulos
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Milioni, D., Hatzopoulos, P. Genomic organization of hsp90 gene family in Arabidopsis. Plant Mol Biol 35, 955–961 (1997). https://doi.org/10.1023/A:1005874521528

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