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Transesterification of phosphatidylcholine with eicosapentaenoic acid ethyl ester using phospholipase A2 in organic solvent

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Abstract

Phospholipase A2-catalysed transesterification of phosphatidylcholine (PC, 99%) with eicosapentaenoic acid ethyl ester (EPAEE, 95%) was carried out in organic solvent. The maximum yield was 14.3% (w/w). The optimum reaction condition was 50 °C, 48 h, initial water activity 0.25 and molar ratio of PC to EPAEE 1:10 in 5 ml toluene.

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Authors and Affiliations

  1. Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1, Kusong-dong, Yusong-gu, Taejon, 305-701, Korea

    Chang Won Park & Joon Shick Rhee

  2. Korea Research Institute of Bioscience & Biotechnology, 52 Oun-dong, Yusong-gu, Taejon, 305-333, Korea

    Seok Joon Kwon

  3. Doosan Venture BG Biotech., 69-1, Sungbok-ri, SuJi-eup, YongIn-si, Kyung gi-do, 449-840, Korea

    Jeong Jun Han

Authors
  1. Chang Won Park
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  2. Seok Joon Kwon
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  3. Jeong Jun Han
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  4. Joon Shick Rhee
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Park, C.W., Kwon, S.J., Han, J.J. et al. Transesterification of phosphatidylcholine with eicosapentaenoic acid ethyl ester using phospholipase A2 in organic solvent. Biotechnology Letters 22, 147–150 (2000). https://doi.org/10.1023/A:1005626508891

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  • DOI: https://doi.org/10.1023/A:1005626508891

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