Abstract
Beet molasses successfully replaced glucose as sole carbon source to produce poly-β-hydroxybutyrate by a recombinant Escherichia coli strain (HMS174/pTZ18u-PHB). The fermentation with molasses was cheaper than with glucose. The final dry cell weight, PHB content and PHB productivity were 39.5 g/L, 80% (w/w) and 1 g/Lh, respectively, in a 5 L stirred tank fermenter after 31.5 h fed-batch fermentation with constant pH and dissolved O2 content. © Rapid Science Ltd. 1998
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References
Beaulieu, M, Beaulieu, Y, Mélinard, J, Pandian, S and Goulet, J (1995). Appl Environ Microbiol 61:65–169
Bradel, R and Reichert, K-H (1993). Makromol Chem 194:1983–1990
Choi, J-I and Lee, SY (1997). Bioprocess Eng 17:335–342
Kim, BS, Lee, SY and Chang, HN (1992). Biotechnol Bioeng 14:811–816
Lee, SY, Middelberg, APJ and Lee, YK (1997). Biotechnol Lett 19:1033–1035
Lee, SY, Yim, KS and Chang, HN (1994). J Biotechnol 32:203–211
Li, HQ, Sha, L, Wang, LN and Shen, ZY (1997). Proceedings of the 4th Asia-Pacific Biochemical Engineering Conference. Beijing, P. R. China, pp 248–351
Schubert, P, Kruger, N and Steinbúchel, A (1991). J Bacteriol 173:168–175
Suzuki, T, Yamane, T and Shimizu, S (1986). Appl Microbiol Biotechnol 23:322–329
Zhang, LX (1986). Experimental Methods and Techniques in Biochemistry. pp 9–11, Beijing, P R China: People's Education Press
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Liu, F., Li, W., Ridgway, D. et al. Production of poly-β-hydroxybutyrate on molasses by recombinant Escherichia coli. Biotechnology Letters 20, 345–348 (1998). https://doi.org/10.1023/A:1005367011378
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DOI: https://doi.org/10.1023/A:1005367011378