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An isolation procedure for arachidonic acid producing Mortierella species

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Abstract

Malt extract agar and an incubation temperature of 5 °C were used to selectively isolate representatives of the genus Mortierella from soil. Fungi in a soil sample from mountain grassland able to grow under these conditions, amounted to a total of 2640 colony forming units per gram soil. Circa 94% of the total fungal isolates represented Mortierella subgenus Mortierella. The rest of the colony-forming units consisted of Mucor isolates (6.0%) and higher fungi (1.5%). All the Mortierella isolates produced arachidonic acid.

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Correspondence to Alfred Botha.

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Botha, A., Paul, I., Roux, C. et al. An isolation procedure for arachidonic acid producing Mortierella species. Antonie Van Leeuwenhoek 75, 253–256 (1999). https://doi.org/10.1023/A:1001848709005

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