Abstract
Fifty-one yeast strains isolated from fermented mash of Balinese rice wine, brem, fermented using five different types of starters, ragi tape, were identified on the basis of their internal transcribed spacer (ITS) regions and their 18S rDNA sequences. The results revealed that Saccharomyces cerevisiae(35 strains), Candida glabrata(six strains), Pichia anomala(three strains) and Issatchenkia orientalis(seven strains) were the main yeasts in the fermentation of the rice wine. These yeasts undergo succession during the fermentation in which S. cerevisiae was mostly found as the principal yeast at the end of fermentation. Phylogenetic analysis based on the 18S rDNA sequences of selected strains placed the isolated S. cerevisiae strains in the Saccharomyces sensu stricto group. Karyotype analysis of the S. cerevisiae strains resolved using pulsed field gel electrophoresis (PFGE) showed that the strains are typically associated with different types of starters.
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References
Ardana, M.M. & Fleet, G.H. 1989 The microbial ecology of tape ketan fermentation. International Journal of Food Microbiology 9, 157–165.
Birren, B. & Lai, E. 1993 Pulsed Field Gel Electrophoresis. A Practical Guide. New York: Academic Press, Inc. ISBN 0-12-101290-5.
Bruns, T.D., White, T.J. & Taylor, J.W. 1991 Fungal molecular systematics. Annual Reviews of Ecology and Systematics 22, 525–564.
Cronk, T.C., Steinkraus, K.H., Hackler, L.R. & Mattick, L.R. 1977 Indonesian tape ketan fermentation. Applied and Environmental Microbiology 33, 1067–1073.
Cronk, T.C., Mattick, L.R., Steinkraus, K.H. & Hackler, L.R. 1979 Production of higher alcohols during Indonesian tape ketan fermentation. Applied and Environmental Microbiology 37, 892–896.
Derrick, S. & Large, P.J. 1993 Activities of the enzymes of the Ehrlich pathway and formation of branched-chain alcohols in Saccharomyces cerevisiae and Candida utilis grown in continuous culture on valine or ammonium as sole nitrogen source. Journal of General Microbiology 139, 2783–2792.
Djien, K.S. 1972 Tape fermentation. Applied Microbiology 23, 974–978.
Dwidjoseputro, D. & Wolf, F.T. 1970 Microbiological studies of Indonesian fermented foodstuffs. Mycopathologia et Mycologia Applicata 41, 211–222.
Fincham, J.R.S., Day, P.R. & Radford, A. 1997 Fungal Genetics, 4th edn. pp. 178–196. Oxford: Blackwell Scientific Publications. ISBN 0-632-00215-8.
Heerde, E. & Radler, F. 1978 Metabolism of the anaerobic formation of succinic acid by Saccharomyces cerevisiae. Archives of Microbiology 117, 269–276.
Hesseltine, C.W. 1965 A millennium of fungi, food, and fermentation. Mycologia 2, 149–198.
Hesseltine, C.W. 1983 Microbiology of oriental fermented foods. Annual Reviews of Microbiology 37, 575–601.
Hoshi, S., Sakata, T., Mikuni, K., Hashimoto, H. & Kimura, S. 1994 Galactosylsucrose and xylosylfructosidase alter digesive tract size and concentrations of cecal organic acids in rats fed diets containing cholesterol and cholic acid. Journal of Nutrition 124, 52–60.
Kuriyama, H., Satraatmadja, D., Igosaki, Y., Watanabe, K., Kanti, A. & Fukatsu, T. 1997 Identification and characterization of yeast isolated from Indonesian fermented food. Mycoscience 38, 441–445.
Longo, E. & Vezinhet, F. 1993 Chromosomal rearrangements during vegetative growth of a wild strain of Saccharomyces cerevisiae. Applied and Environmental Microbiology 59, 322–326.
Molina, F.I., Inoue, T. & Jong, S.C. 1992a Restriction polymorphisms in the internal transcribed spacers and 5.8S rDNA of Saccharomyces. Current Microbiology 25, 251–255.
Molina, F.I., Inoue, T. & Jong, S.C. 1992b Ribosomal DNA restriction analysis reveals genetic heterogeneity in Saccharomyces cerevisiae Meyen ex Hansen. International Journal of Systematic Bacteriology 42, 499–502.
Radler, F. 1990 Yeast-metabolism of organic acids. In Wine Microbiology and Biotechnology, ed. Fleet, G.H. pp. 165–182. Switzerland: Harwood Academic Publishers GmbH. ISBN 3-7186-5132-7.
Shimizu, Y. & Watanabe, M. 1981 Effects of yeasts strains and environmental conditions on formation of organic acids in must during fermentation. Journal of Fermentation Technology 59, 27–32.
Shin, K.S., Hong, S.D. & Bae, K.S. 1996 The significance of ITSRFLPs and coenzyme Q system in determining taxonomic relationships among Candida species. Journal of General and Applied Microbiology 42, 481–491.
Steinkraus, K.H. 1996 Indigenous fermented foods in which ethanol is a major product. In Handbook of Indigenous Fermented Foods, 2nd edn. ed. Steinkraus, K.H. pp. 363–508. NewYork: Marcel Dekker, Inc. ISBN 0-8247-9352-8.
Sujaya, I.N., Amachi, S., Saito, K., Yokota, A., Asano, K. & Tomita, F. 2002 Specific enumeration of lactic acid bacteria in ragi tape by colony hybridization with specific oligonucleotide probes. World Journal of Microbiology and Biotechnology 18, 263–270.
White, T.J., Bruns, T., Lee, S. & Taylor, J. 1990 Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR Protocols. A Guide to Methods and Applications, eds. Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. pp. 315–322. San Diego: Academic Press, Inc. ISBN 0-12-372181-4.
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Sujaya, I., Antara, N., Sone, T. et al. Identification and characterization of yeasts in brem, a traditional Balinese rice wine. World Journal of Microbiology and Biotechnology 20, 143–150 (2004). https://doi.org/10.1023/B:WIBI.0000021727.69508.19
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DOI: https://doi.org/10.1023/B:WIBI.0000021727.69508.19