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A novel strain of Streptomyces malaysiensis isolated from Brazilian soil produces high endo-β-1,4-xylanase titres

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Abstract

One hundred and sixty two actinomycete strains isolated from Brazilian soils were screened for xylanase activity, according to the size of the hydrolysis zones observed in oat spelts xylan agar plates. The strain AMT-3, later identified as Streptomyces malaysiensis, was selected as the best producer. In subsequent shake flasks fermentations using growth media contanning 1% (w/v) of either birchwood, or oat spelts xylan, plus organic nitrogen and salts, high endo-β-1,4-xylanase titres (EC 3.2.1.8) (116 U ml−1) were observed in the larchwood medium within 6 days. This is the first report concerning xylanase production by streptomyces malaysiensis, which has been recently described as a new species.

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Authors and Affiliations

  1. Instituto de Microbiologia Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro, Av. Brigadeiro Trompowski, s/n CCS – Bloco I, Cidade Universitária, CEP 21941-590, Rio de Janeiro, RJ, Brazil

    Rodrigo Pires do Nascimento & Doralice Rodrigues Sacramento

  2. Departamento de Biotecnologia, Instituto Nacional de Engenharia e Tecnologia Industrial, Lisboa, Portugal

    Susana Marques, Luís Alves, Francisco Gírio & Maria Teresa Amaral-Collaço

  3. Instituto de Quimica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil

    Elba Pinto da Silva Bon & Rosalie Reed Rodrigues Coelho

Authors
  1. Rodrigo Pires do Nascimento
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  2. Susana Marques
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  3. Luís Alves
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  4. Francisco Gírio
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  5. Maria Teresa Amaral-Collaço
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  6. Doralice Rodrigues Sacramento
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  7. Elba Pinto da Silva Bon
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  8. Rosalie Reed Rodrigues Coelho
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do Nascimento, R.P., Marques, S., Alves, L. et al. A novel strain of Streptomyces malaysiensis isolated from Brazilian soil produces high endo-β-1,4-xylanase titres. World Journal of Microbiology and Biotechnology 19, 879–881 (2003). https://doi.org/10.1023/B:WIBI.0000007287.03281.dd

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  • DOI: https://doi.org/10.1023/B:WIBI.0000007287.03281.dd

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