Abstract
Organophosphate insecticide (parathion/diazinon) resistance in housefly (Musca domestica L.) is associated with the change in carboxylesterase activity. The product of MdαE7 gene is probably playing a role in detoxification of xenobiotic esters. In our research, we have isolated, cloned and sequenced the MdαE7 gene from five different Turkish housefly strains. High doses of malathion (600 μg/fly) were applied in a laboratory environment for one year to Ceyhan1, Ceyhan2, Adana, and Ankara strains while no insecticide treatment was performed in the laboratory to Kirazli strain. Trp251 → Ser substitution was found in the product of MdαE7 gene in all malathion-resistant and Kirazli stocks. In addition, we checked the malathion carboxylesterase (MCE), percent remaining activities in acetylcholinesterase (AChE), glutathion-S-transferase (GST), and general esterase activities in all five strains used in this study. In comparing with universal standard sensitive control WHO, a high level of MCE and GST activities were observed while lower level of general esterase activities was detected in the tested strains. In addition, a higher percent remaining activities in AChE than WHO susceptible strain were observed in all malathion-resistant strains.
Similar content being viewed by others
REFERENCES
Lockridge, O., Blong, R.M., Masson, P.,et al.,A single amino acid substitution,Gly117 → His,Confers Phosphotriesterase (Organophosphorus Acid Anhydride Hydrolase)Activity on Human Butyrylcholinesterase,Biochemistry, 1997,vol.31,pp.991–997.
Claudianos, C., Crone, E., Coppin, C.,et al.,A Genomics Perspective on Mutant Aliesterases and Metabolic Resistance to Organophosphate,Agrochemical Resis-tance:Extent,Mechanism and Detection,Marchall, C.J. and Yamaguchi, I.,Eds.,WA (USA): Am.Chem.Soc., 2002,pp.90–101.
Campbell, P.M., Newcomb, R.D., Russell, R.J.,and Oakeshott, J.G.,Two Different Amino Acid Substitutions in the Ali-Esterase,E3,Confer Alternative Types of Organophosphorus Insecticide Resistance in the Sheep Blowfly,Lucilia cuprina,Insect Biochem.Mol. Biol.,1998,vol.28,pp.139–150.
Campbell, P.M., Trott, J.F., Claudianos, C.,et al.,Biochemistry of Esterases Associated with Organophosphate Resistance in Lucilia cuprina with Comparisons to Putative Orthologues in Other Diptera,Biochem.Genet., 1997,vol.35,pp.17–40.
Devonshire, A.L.and Field, L.M.,Gene Amplification and Insecticide Resistance,Annu.Rev.Entomol.,1991, vol.36,pp.1–23.
Hemingway, J.,The Molecular Basis of Two Contrasting Metabolic Mechanisms of Insecticide Resistance,Insect Biochem.Mol.Biol.,2000,vol.30,pp.1009–1015.
Brownlie, J.,The Evolution of Diazinon Resistance Alleles,and Their Impact on the Genetic Diversity of the α-Esterase Cluster of the Common Housefly,Musca domestica,Honors Thesis, Canberra: Austral.Nat.Univ., 1996.
Claudianos, C., Russell, R.J.,and Oakeshott, J.G.,The Same Amino Acid Substitution in Orthologous Esterases Confers Organophosphate Resistance on the Housefly and a Blowfly,Insect Biochem.Mol.Biol.,1999,vol.29, pp.675–686.
Campbell, P.M., Yen, J.L.,and Masoumi, A.,Cross-Resistance Patterns among Lucilia cuprina (Diptera:Calliphoridae)Resistant to Organophosphorus Insecticides, J.Economic Entomol.,1998,vol.91,no.2,pp.367–375.
Oakeshott, J.G., Claudianos, C., Russell, R.J.,and Robin, G.C.,Carboxyl/Cholinesterases:A Case Study of the Evolution of a Successful Multigene Family, BioEssays, 1999,vol.21,pp.1031–1042.
Newcomb, R.D., Campbell, P.M., Russell, R.J.,and Oakeshott, J.G.,cDNA Cloning,Baculovirus Expression and Kinetic Properties of the Esterase,E3,Involved in Organophosphorus Insecticide Resistance in Lucila cuprina,Insect Biochem.Mol.Biol.,1996,vol.27, pp.15–25.
Newcomb, R.D., Campbell, P.M., Ollis, D.L.,et al., A Single Amino Acid Substitution Converts a Carboxylesterase to an Organophosphorus Hydrolase and Confers Insecticide Resistance on a Blowfly,Proc.Natl.Acad.Sci.USA, 1997,vol.94,pp.7464–7468.
Oppenoorth, F.J.and Van Asperen, K.,Allelic Genes in the Housefly Producing Modified Enzymes That Cause Organophosphate Resistance,Science, 1960,vol.132, pp.298–299.
Clark, A.G.and Shamaan, N.A.,Evidence That DDT-Dehydrochlorinase from the Housefly Is a Glutathione S-Transferase,Pesticide Biochem.Physiol.,1984,vol.22, pp.249–261.
Zhou, H.A.and Syvanen, M.,A Complex Glutathione Transferase Gene Family in the Housefly Musca domestica,Mol.Gen.Genet.,1997,vol.256,pp.187–194.
Hemingway, J., Miyamoto, J.,and Herath, P.R.,A Possible Link between Organophosphorus and DDT Insecticide Resistance Genes in Anopheles:Supporting Evidence from Fenitrothion Metabolism Studies,Pesticide Biochem.Physiol.,1991,vol.39,pp.49–56.
Wang, J., McCommas, S.,and Syvanen, M.,Molecular Cloning of a Glutathione S-Transferase Overproduced in an Insecticide Resistant Strain of the Housefly (Musca domestica ),Mol.Gen.Genet.,1991,vol.277,pp.260–266.
Wei, S.H., Clark, A.G.,and Syvanen, M.,Identification and Cloning of a Key Insecticide Metabolizing Glutathione S-Transferase (MdGST-6A)from a Hyper Insecticide-Resistant Strain of the Housefly Musca domestica,Insect Biochem.Mol.Biol.,2001,vol.31,pp.1145–1153.
Grant, D.F.and Hammock, D.D.,Genetics and Molecular Evidence for a Transacting Regulatory Locus Controlling Glutathione S-Transferase-2 Expression in Aedes aegyptii,Mol.Gen.Genet.,1992,vol.234,pp.169–176.
Oppenoorth, F.J.,Biochemistry of Insecticide Resistance,Pesticide Biochem.Physiol.,1984,vol.22, pp.187–193.
Devonshire, A.L.and Moores, G.D.,Different Forms of Insensitive Acetylcholinesterase in Insecticide Resistant House Flies (Musca domestica ),Pesticide Biochem. Physiol.,1984,vol.21,pp.336–340.
Moores, G.D., Devonshire, A.L.,and Denholm, I., A Microtitre Plate Assay for Characterizing Insensitive Acetylcholinesterase Genotypes of Insecticide-Resistant Insects,Bull.Entomol.Res.,1988,vol.78,pp.537–544.
Kozaki, T., Shono, T., Tomita, T.,and Kono, Y.,Fenitroxon Insensitive Acetylcholinesterases of the Housefly,Musca domestica, Associated with Point Mutations, Insect Biochem.Mol.Biol.,2001,vol.31,pp.991–997.
Chen, Z., Newcomb, R., Forbes, E.,et al.,The acetylcholinesterase gene and organophosphorus resistance in the Australian sheep blowfly,Lucilia cuprina,Insect Biochem.and Mol.Biol.,2001,vol.31,pp.805–816.
Charpentier, A., Menozzi, P., Veronique, M.,et al., A Method to Estimate Acetylcholinesterase Active Sites and Turnover in Insects,Anal.Biochem.,2000,vol.285, pp.76–81.
Yao, H., Chuanling, Q., Williamson, M.S.,and Devonshire, A.L.,Characterization of the Acetylcholinesterase Gene from Insecticide Resistant Houseflies (Musca domestica ),Chinese J.Biotechnol.,1997,vol.13,pp.177–183.
Claudianos, C.,The Evolution of α-Esterase Mediated Organophosphate Resistance in Musca domestica,PhD Thesis,Canberra: Austral.Nat.Univ.,1999.
Bender, W., Spierer, P.,and Hogness, D.S.,Chromosomal Walking and Jumping to Isolate DNA from theAce and rosy Loci and bithorax Complex in Drosophila melanogaster,J.Mol.Biol.,1983,vol.168,pp.17–33.
Sambrook, J., Fritch, E.F.,and Maniatis, T.,Molecular Cloning:A Laboratory Manual,Cold Spring Harbor, New York: Cold Spring Harbor Lab.,1989.
Habig, W.H., Pabst, M.J.,and Jakobi, B.W.,Glutathione S-Transferases,J.Biol.Chem.,1974,vol.249,pp.7130–7139.
Gomori, G.,Human Esterases,J.Lab.Clin.Med.,1953, vol.42,pp.445–453.
Bradford, M.M.,A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein–Dye Binding,Anal.Biochem.,1976,vol.72,pp.248–254.
Devereux, J., Haeberli, P.,and Smithies, O.,A Comprehensive Set of Sequence Analysis Programs for the Vax, Nucleic Acids Res.,1984,vol.12,pp.387–395.
Cygler, M., Schrag, J.D., Sussman, J.L.,et al.,Relationship between Sequence Conservation and Three-Dimensional Structure in a Large Family of Esterases,Lipases and Related Proteins,Protein Sci.,1993,no.2,pp.366–382.
Robin, C., Russell, R.J., Medveczky, K.M.,and Oakeshott, J.G.,Duplication and Divergence of the Genes of the áα-Esterase Cluster of Drosophila melanogaster,J.Mol. Evol.,1996,vol.43,pp.241–252.
Feyereisen, R.,Insect P450 Enzymes,Annu.Rev.Entomol.,1999,vol.44,pp.507–533.
Plapp, F.W.,The Genetic Basis of Insecticide Resistance in the Housefly:Evidence That a Single Locus Plays a Major Role in Metabolic Resistance to Insecticides,Pesticide Biochem.Physiol.,1984,vol.22,pp.194–201.
Franciosa, H.and Berge, J.B.,Glutathione S-Transferase in Housefly Musca domestica L.:Location of GST-1 and GST-2 Families,Insect Biochem.Mol.Biol.,1995,vol. 25,pp.311–317.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Tas¸kin, V., Kence, M. The Genetic Basis of Malathion Resistance in Housefly (Musca domestica L.) Strains From Turkey. Russian Journal of Genetics 40, 1215–1222 (2004). https://doi.org/10.1023/B:RUGE.0000048663.17417.97
Issue Date:
DOI: https://doi.org/10.1023/B:RUGE.0000048663.17417.97