Skip to main content
SpringerLink
Log in

Streptozotocin-induced type 1 diabetes mellitus alters the morphology, secretory function and acyl lipid contents in the isolated rat parotid salivary gland

  • Published:
Molecular and Cellular Biochemistry Aims and scope Submit manuscript

Abstract

Diabetes mellitus (DM) is associated with numerous conditions including hypo-secretion of digestive enzymes. This study investigated the morphology, secretory function (α-amylase release) and acyl lipid contents in the isolated parotid gland of STZ-induced diabetic and age-matched control rats in order to provide insights into diabetes-induced salivary insufficiency. The techniques employed included light microscopy, colourimetric and gas chromatography (GC) analysis, respectively. Diabetes mellitus was induced in adult male Wistar rats by a single intraperitoneal (IP) injection of streptozotocin (STZ) (60 mg per kg body weight). Control animals were injected with a similar volume of citrate buffer. The animals were tested for DM 4 days after STZ injection and 2 months later when they were humanely killed for the experiment. The morphological results showed diabetic parotid glands to be extensively infiltrated with lipid droplets of various magnitudes, whereas glands from control animals display normal structure with the absence of lipid droplets. The analysis of parotid secretory function revealed a significant (p < 0.05) dose-dependent decrease in α-amylase release in response to noradrenaline (NA) in STZ-treated glands when compared to age-match control parotid glands. Furthermore, the levels of acyl lipids (16:0, 16:1, 18:0 and 18:1) in diabetic parotid glands was significantly (p < 0.01) reduced compared to control glands, along with a reduced ratio of 16:1/16:0. The results indicate DM can elicit changes in the morphology, secretory function and acyl fatty acid quantity in the isolated rat parotid gland. (Mol Cell Biochem 261: 175–181, 2004)

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Williams G, Pickup JC: Handbook of Diabetes. Cambridge University Press, Cambridge, UK, 1998

    Google Scholar 

  2. Petersen OH: Stimulus-secretion coupling: Cytoplasmic calcium signals and the control of ion channels in exocrine acinar cells. J Physiol 448: 1–51, 1992

    CAS  PubMed  Google Scholar 

  3. Schultz I: Stimulus-secretion coupling in exocrine glands: Role of 1,4,5-trisphosphate, calcium and cAMP. Curr Eye Res 4(4): 467–473, 1985

    Google Scholar 

  4. Putney JW: Identification of cellular activation mechanisms associated with salivary secretion. Ann Rev Physiol 48: 75–88, 1986

    CAS  Google Scholar 

  5. Dang AQ, Faas FH, Lee JA, Carter WJ: Altered fatty acid composition in the plasma, platelets, and aorta of the streptozotocin-induced diabetic rat. Metabolism 37: 1065–1072, 1988

    Article  CAS  PubMed  Google Scholar 

  6. Igal RA, Mandon EC, Des Gomez Dumm IN: Abnormal metabolism of polyunsaturated fatty acids in adrenal glands of the diabetic rat. Mol Cell Endocrinol 77: 217–227, 1991

    Article  CAS  PubMed  Google Scholar 

  7. Shin CS, Lee MK, Park KS, Kim SY, Cho BY, Lee HK, Koh CS, Min HK: Insulin restores fatty acid composition earlier in liver microsomes than erythrocytes membranes in streptozotocin-induced diabetic rats. Diabetes Res Clin Pract 29: 93–98, 1995

    Article  CAS  PubMed  Google Scholar 

  8. Chorvathova V, Ondreicka R: The fatty acid composition of the tissues of streptozotocin-diabetic rats. Physiol Bohemoslov 32(5): 466–475, 1983

    CAS  PubMed  Google Scholar 

  9. Ghebremeskel K, Bitsanis D, Koukkou E, Lowry C, Poston L, Crawford MA: Liver triacylglycerols and free fatty acids in streptozotocin-induced diabetic rats have atypical n-6 and n-3 pattern. Comp Biochem Physiol C 132: 349–354, 2002

    CAS  Google Scholar 

  10. Sharma AK, Duguid IGM, Blanchard DS, Thomas PK: The effect of insulin treatment on myelinated nerve fibre maturation and integrity and on body growth in streptozotocin-diabetic rats. J Neurol Sci 67: 285–297, 1985

    Article  CAS  PubMed  Google Scholar 

  11. Adeghate EA, Singh J, Burrows S, Howarth FC, Donath T: Secretory responses and peptidergic and aminergic innervation of the rat lacrimal glands. Biogenic Amines 10(2): 487–498, 1994

    CAS  Google Scholar 

  12. Draper CE, Adeghate EA, Singh J, Pallot DJ: Evidence to suggest morphological and physiological alterations of lacrimal gland acini with ageing. Exp Eye Res 68: 265–276, 1999

    Article  CAS  PubMed  Google Scholar 

  13. Gonzalez A, Camello PJ, Pariente JA, Salido GM: Free cytosolic calcium levels modify intracellular pH in rat pancreatic acini. Biochem Biophys Res Comm 230: 652–656, 1997

    Article  CAS  PubMed  Google Scholar 

  14. Herzog V, Sies H, Miller F: Exocytosis in secretory cells of rat lacrimal gland. J Cell Biol 70: 692–706, 1976

    Article  CAS  PubMed  Google Scholar 

  15. Gardner JD, Jackson MJ: Regulation of amylase release from dispersed pancreatic acinar cells. J Physiol 270: 439–454, 1977

    CAS  PubMed  Google Scholar 

  16. Ceska M, Birath K, Brown B: A new and rapid method for the clinical determination of α-amylase activities in human serum and urine. Optimal Conditions. Clin Chem Acta 26: 437–444, 1969

    CAS  Google Scholar 

  17. Rolph EC, Goad LJ: Phosphatidylcholine biosynthsesis in celery cell suspension cultures with altered sterol composistions. Physiol Plant 83: 605–610, 1991

    Article  CAS  Google Scholar 

  18. Levy E, Roy CC, Lepage G, Bendayen M: Lipid abnormalities in pancreatic tissue of streptozotocin-induced diabetic rats. Lipids 23: 771–778, 1988

    CAS  PubMed  Google Scholar 

  19. Reuterving CO, Hagg E, Henriksson R, Holm J: Salivary glands in long-term alloxan-diabeticrats. Aquantitative light and electron-microscopic study. Acta Pathol Microbiol Immunl Scan 95(3): 131–136, 1987

    CAS  Google Scholar 

  20. Anderson LC, Garrett JR: Lipid accumulation in the major salivary glands of streptozotocin-diabetic rats. Archs Oral Biol 31(7): 469–475, 1986

    CAS  Google Scholar 

  21. Komabayashi T, Ikeda T, Suda K, Izawa T: Beta-adrenergic receptors and adenylate cyclase activity in the parotid acinar cells from acute streptozotocin-induced diabetic rats. Res Commun Mol Pathol Pharmacol 107(3/4): 311–322, 2000

    CAS  PubMed  Google Scholar 

  22. Anderson LC: Parotid gland function in streptozotocin-diabetic rats. J Dent Res 66(2): 425–429, 1987

    CAS  PubMed  Google Scholar 

  23. Szczepanski A, Mednieks MI, Hand AR: Expression and distribution of parotid secretory proteins in experimental diabetes. Eur J Morphol 36: 240–246, 1998

    PubMed  Google Scholar 

  24. Kim SK, Cuzzort LM, Mckean RK, Allen ED: Effects of diabetes and insulin on alpha-amylase messenger RNA levels in rat parotid glands. J Dent Res 69(8): 1500–1504, 1990

    CAS  PubMed  Google Scholar 

  25. Hand AR, Weiss Re: Effects of streptozotocin-induced diabetes on the rat parotid gland. Lab Invest; J Tech Methods Pathol 51(4): 429–440, 1984.

    CAS  Google Scholar 

  26. Kluber KM, Feczko JD: Ultrastructural, histochemical, and morphometric analysis of skeletal muscle in murine model of type 1 diabetes. Anat Rec 239(1): 18–34, 1994

    Google Scholar 

  27. Abdel-Rahman MS, Elrakhawy FI, Iskander FA: Endocrine pancreas in postnatal offspring of alloxan diabetic rats. Toxicol Lett 62(2/3): 263–274, 1992

    CAS  PubMed  Google Scholar 

  28. Gewolb IH, Torday JS: High glucose inhibits maturation of the fetal lung in vitro: Morphometric analysis of lamellar bodies and fibroblast lipid inclusions. Lab Invest 73(1): 59–63, 1995

    CAS  PubMed  Google Scholar 

  29. Moir AMB, Zammit VA: Effects of insulin treatment of diabetic rats on hepatic partitioning of fatty acids between oxidation and esterifiction, phospholipids and acylglycerol synthesis, and on the fractional rate of secretion of triacylglycerol in vivo. Biochem J 304(1): 177–182, 1994

    CAS  PubMed  Google Scholar 

  30. Giron MD, Sanchez F, Hortelano P, Periago JL, Suarez MD: Effects of dietry fatty acids on lipid metabolism in streptozotocin-induced diabetic rats. Met Clin Exp 48(4): 455–460, 1999

    CAS  Google Scholar 

  31. Mimouni V, Poisson JP: Altered desaturase activities and fatty acid composition in liver microsomes of spontaneously diabetic Wistar BB rats. Biochem Biophys Acta-Lipid. Lipid Met 1123(3): 296–302, 1992

    CAS  Google Scholar 

  32. Morris PA, Prout RES, Proctor GB, Garrett JR, Anderson LC: Lipid analysis of the major salivary glands in streptozotocin-diabetic rats and the effects of insulin treatment. Archs Oral Biol 37(6): 489–494, 1992

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biological Sciences, University of Central Lancashire, Preston, England, UK

    S. Mahay, M. Z. Lindley, C. E. Rolph & J. Singh

  2. Department of Human Anatomy, F.M.H.S., United Arab Emirates University, Al-Ain, UAE

    E. Adeghate

Authors
  1. S. Mahay
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. Adeghate
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Z. Lindley
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. C. E. Rolph
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. J. Singh
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mahay, S., Adeghate, E., Lindley, M.Z. et al. Streptozotocin-induced type 1 diabetes mellitus alters the morphology, secretory function and acyl lipid contents in the isolated rat parotid salivary gland. Mol Cell Biochem 261, 175–181 (2004). https://doi.org/10.1023/B:MCBI.0000028753.33225.68

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/B:MCBI.0000028753.33225.68

Search

Navigation