biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 48:477-479, 2004 | DOI: 10.1023/B:BIOP.0000041109.27037.21

Cytokinin-Mediated Axillary Shoot Formation in Pinus heldreichii

D. Stojičić1, S. Budimir2
1 Institute of Forestry, Belgrade, Serbia and Montenegro, India, e-mail
2 Institute for Biological Research "Siniša Stanković", Belgrade, Serbia and Montenegro, India

Shoot formation from seedling explants of Pinus heldreichii was induced by pulse treatment with benzyladenine at different concentration, followed by culture on medium lacking plant growth regulators. After treatment with 222 μM benzyladenine (BA) an average of 4.6 shoots per explant was obtained. Shoots, detached from explants, rooted with a frequency of about 10 %, and rooted plantlets were successfully transferred to ex vitro conditions.

Keywords: benzyladenine; pine; plant regeneration; seedling explant
Subjects: cytokinins; in vitro culture, regeneration, organogenesis; nutrient medium, GD (Gresshoff and Doy); pine, seedling explant, regeneration; Pinus heldreichii; shoot formation, axillary

Published: September 1, 2004  Show citation

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Stojičić, D., & Budimir, S. (2004). Cytokinin-Mediated Axillary Shoot Formation in Pinus heldreichii. Biologia plantarum, 48(3), 477-479. doi: 10.1023/B:BIOP.0000041109.27037.21
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