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Combining induced mutation and protoplasting for strain improvement of Aspergillus oryzae for kojic acid production

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Abstract

By combining induced mutation, using NTG and UV irradiation, and protoplasting of a wild type strain of Aspergillus oryzae ATCC 22788, a hyper-producing strain was obtained that accumulated 41 g kojic acid l−1 in shake-flasks, which was 100-fold higher than that in the wild type strains. Similar production of kojic acid was obtained in 5 l stirred-tank fermentations.

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Authors and Affiliations

  1. Department of Chemical Engineering, National Tsing Hua University, Hsinchu, 30043, Taiwan

    Hisao-Ming Wan, Chien-Chiang Chen, Te-Sheng Chang, Raghavan N. Giridhar & Wen-Teng Wu

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  1. Hisao-Ming Wan
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  2. Chien-Chiang Chen
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  3. Te-Sheng Chang
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  4. Raghavan N. Giridhar
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  5. Wen-Teng Wu
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Correspondence to Wen-Teng Wu.

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Wan, HM., Chen, CC., Chang, TS. et al. Combining induced mutation and protoplasting for strain improvement of Aspergillus oryzae for kojic acid production. Biotechnology Letters 26, 1163–1166 (2004). https://doi.org/10.1023/B:BILE.0000035490.49252.38

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  • DOI: https://doi.org/10.1023/B:BILE.0000035490.49252.38

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