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Human Aurora-B binds to a proteasome α-subunit HC8 and undergoes degradation in a proteasome-dependent manner

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Abstract

Human Aurora/Ipl1-related kinase 2 (Aurora-B) is a key regulator of mitosis. Here human proteasome α-subunit C8 (HC8) was identified to interact with the Aurora-B by yeast two-hybrid screen. This finding was confirmed by GST pull-down assays and immunoprecipitation experiments. The Aurora-B protein level increased in HeLa cells cultured with proteasome inhibitor ALLN. Our data suggest that Aurora-B might undergo degradation by binding to HC8 in a proteasome-dependent manner during mitosis.

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Authors and Affiliations

  1. State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai, 200433, P.R. China

    Fengjue Shu, Shuguang Guo, Yongjun Dang, Meiyan Qi, Guangjin Zhou, Zekun Guo, Ying Zhang, Chaoqun Wu, Shouyuan Zhao & Long Yu

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  1. Fengjue Shu
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  2. Shuguang Guo
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  3. Yongjun Dang
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  4. Meiyan Qi
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  6. Zekun Guo
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Shu, F., Guo, S., Dang, Y. et al. Human Aurora-B binds to a proteasome α-subunit HC8 and undergoes degradation in a proteasome-dependent manner. Mol Cell Biochem 254, 157–162 (2003). https://doi.org/10.1023/A:1027317014159

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