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Enhanced salidroside production in liquid-cultivated compact callus aggregates of Rhodiola sachalinensis: manipulation of plant growth regulators and sucrose

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Abstract

Liquid-cultivated compact callus aggregates (CCA) of Rhodiola sachalinensis were initiated from the explant of stems. Microscopic analysis showed that CCA displayed some level of cellular/tissue differentiation, which was perhaps responsible for the increased salidroside production. A higher ratio of cytokinin/auxin was found to be beneficial both for CCA growth and salidroside accumulation. Sucrose (> 100 g l-1) had a positive effect on salidroside synthesis, which was not due to an osmotic effect of the medium. The medium containing 40 g l-1 sucrose, 1.5 μM naphthaleneacetic acid and 15 μM 6-benzylaminopurine yielded ca. 120 mg l-1 salidroside after 24 days of culture.

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Authors and Affiliations

  1. State Key Laboratory of Biochemical Engineering, Institute of Chemical Metallurgy, Chinese Academy of Science, Beijing, 100080, P. R. China

    J. F. Xu & Z. G. Su

  2. Institute of Biochemical Engineering, Dalian University of Technology, Dalian, 116012, P. R. China

    P. Q. Ying & A. M. Han

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  1. J. F. Xu
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  2. P. Q. Ying
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  3. A. M. Han
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  4. Z. G. Su
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Correspondence to J. F. Xu.

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Xu, J.F., Ying, P.Q., Han, A.M. et al. Enhanced salidroside production in liquid-cultivated compact callus aggregates of Rhodiola sachalinensis: manipulation of plant growth regulators and sucrose. Plant Cell, Tissue and Organ Culture 55, 53–58 (1998). https://doi.org/10.1023/A:1026489515174

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  • DOI: https://doi.org/10.1023/A:1026489515174

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