Abstract
The regeneration potential of D.alata L. germplasm preserved in vitro was compared with the micropropagation of fresh material. Nodal cuttings were conserved for 9 months in different treatments based on D-571 culture medium modified, using several variable components (mannitol, benzylaminopurine and activated charcoal). Regeneration at 8 weeks, assessed by means of percentage of explant regenerating and the multiplication at 5 weeks through the shoot length and de novo bud count formation per explant were determined. The results showed high rates (100 and 98%) of explant regeneration and micropropagation from in vitro material maintained in D-571 medium with 1.5% of mannitol + 0.1 or 1 mg l−1 of benzylaminopurine + 2 g l−1 of activated charcoal, respectively.
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Borges, M., Ceiro, W., Meneses, S. et al. Regeneration and multiplication of Dioscorea alata germplasm maintained in vitro . Plant Cell, Tissue and Organ Culture 76, 87–90 (2004). https://doi.org/10.1023/A:1025804516226
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DOI: https://doi.org/10.1023/A:1025804516226