Abstract
The impact of biosolid and soil type on the movement of faecal coliforms through the vadose zone was investigated following the application of animal manure to soil. Two types of biosolid, solid and liquid manure, were applied to two soil types at a wide range of initial water contents. Bacteria present in the soil solution were collected using calibrated ceramic-porous-cup samplers. Estimated bacterial migration velocities in the soil profile were consistent with the hypothesis that bacteria move mostly through soil macropores, as the rate of transport was faster than the average pore-water velocity. Macropore transport was more likely to occur in wet soils, but it was not necessarily restricted to soils with high initial soil water content. A larger soil clay content, lower total soil porosity, and lower saturated hydraulic conductivity resulted in a greater likelihood that suspended bacteria would be funnelled through pores of larger diameter and faster pore water velocity, increasing the potential vertical transport length of bacteria through the vadose zone. Total porosity was not a significant factor in enhancing deep transport of faecal bacteria. The potential of faecal bacteria to be transported to depth in soil was correlated with the water content of the manure. We conclude that application of animal manure to soil can readily lead to groundwater contamination with faecal bacteria especially under moist soil conditions, and that macropores are important in the transport.
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Acea, M. J., Moore, C. R. and Alexander, M.: 1988, ‘Survival and growth of bacteria introduced into soil’, Soil Biol. Biochem. 20, 509-515.
Barrington, S. and Jutras, P. J.: 1983, ‘Soil sealing by manure in various soil types’, ASAE Winter Meeting, Microfiche collection, fiche No. 83-4571, St. Joseph, MI.
Beven, K. and Germann, P.: 1982, ‘Macropores and water flow in soils’, Water Resour. Res. 18, 1311-1325.
Charriere, G. D., Mossel, A. A., Beaudeau, P. and Leclerc, H.: 1994, ‘Assessment of the marker value of various components of the coli-aerogenes group of Enterobacteriaceae and of a selection of Enterococcus spp. for the official monitoring of drinking water supplies’, J. Appl. Bacteriol. 76, 336-344.
Clesceri, L. S., Greenberg, A. E. and Trussel, R. R.: 1998, Standard Methods for the Examination of Water and Wastewater, 17th ed., American Public Health Association, Washington, DC, 1220 pp.
Cools, D.: 2001, ‘Manure-derived Antibiotic Resistant Bacteria: Survival in Soil and Contamination of Crop Roots’, Ph.D. Thesis, Katholieke Universiteit Leuven, 320 pp.
Dickson, J. S. and Daniels, E. K.: 1991, ‘Attachment of Salmonella typhimurium and Listeria monocytogenes to glass as affected by surface film thickness, cell density, and bacterial motility’, J. Ind. Microbiol. 8, 281-284.
Gerba, C. P. and Bitton, G.: 1984, ‘Microbial Pollutants: Their Survival and Transport Pattern to Groundwater’, in G. Bitton and C. P. Gerba (eds), Groundwater Pollution Microbiology, John Wiley & Sons, New York, NY, U.S.A., pp. 65-88.
Hegde, P. and Kanwar, R. S.: 1997, ‘Impact of Manure Application on Groundwater Quality’, ASAE Annual International Meeting, Paper No. 97-2144 AWE, Minneapolis, MI.
Jacobsen, O. H., Moldrup, P., Larsen, C., Konnerup, L. and Petersen, L.W.: 1997, ‘Particle transport in macropages of undistributed soil columns’, J. Hydrol. 196, 185-203.
Konstantinov, A. R.: 1971, Evaporation in Nature, Israel Program for Scientific Translations, Jerusalem, 200 pp.
Mathess, G., Pekdegger, A. and Schroefer, J.: 1988, ‘Persistence and transport of bacteria and viruses in groundwater - A conceptual evaluation’, J. Contam. Hydrol. 2, 171-188.
McMurry, S. W. Coyne, M. S. and E. Perfect, E.: 1998, ‘Fecal coliform transport through intact soil blocks amended with poultry manure’, J. Environ. Qual. 27, 86-92.
Natsch, A., Keel, C., Troxler, J., Zala, M., Von Albertini, N. and Defago, G.: 1996, ‘Importance of preferential flow and soil management in vertical transport of a biocontrol strain of Pseudomonas fluorescens in structured fields’, Appl. Environ. Microbiol. 62, 33-40.
Patni, N. K., Toxopeus, H. R. and Jui, P. Y.: 1985, ‘Bacterial quality of runoff from manured and non-manured cropland’, Trans. ASAE, 28, 1871-1877.
Rattray, E. A., Prosser, J. I., Glover, L. A. and Killham, K.: 1992, ‘Matric potential in relation to survival and activity of a genetically modified microbial inoculum in soil’, Soil Biol. Biochem. 24, 421-425.
Rüprich, A.: 1994, ‘Felduntersuchungen zum Infiltrationsvermögen und zur Lebensfähigkeit von Fäkalkeimen in Boden nach Gülledüngung’, Ph. D. thesis, University of Hohenheim, 242 pp.
Sjogren, R. E. and Gibson, M. J.: 1981, ‘Bacterial survival in a dilute environment’, Appl. Environ. Microbiol. 41, 1331-1336.
Stotzky, G.: 1985, ‘Mechanisms of Adhesion to Clays, with Reference to Soil Systems’, in D. C. Savage and M. Fletcher (eds), Bacterial Adhesion: Mechanisms and Physiological Significance, Plenum Press, New York, NY, U.S.A., pp. 195-254.
Topp, G. C., Davis, J. L. and Annan, A. P.: 1980, ‘Electromagnetic determination of soil water content: Measurements in coaxial transmission lines’, Water Resour. Res. 16, 574-582.
Unc, A.: 1999, ‘Transport of Faecal Bacteria from Manure through the Vadose Zone’, M.Sc. Thesis, Land Resource Science, University of Guelph, 163 pp.
Unc, A. and Goss, M. J.: 2000, ‘Development of a protocol for sampling faecal coliform bacteria originating from manure in the vadose zone’, Water Qual. Res. J. Can. 35, 23-38.
Wollum II, A. G.: 1986, ‘Cultural Methods for Soil Microorganisms’, in A. Klute, G. S. Campbell, R. D., Jackson, M. M., Mortland and D. R. Nielsen (eds), Methods of Soil Analysis. Part 2 - Chemical and Microbiological Properties, 2nd ed., American Society of Agronomy, Inc., Soil Science Society of America, Inc., Madison, WI., U.S.A., pp. 781-802.
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Unc, A., Goss, M.J. Movement of Faecal Bacteria through the Vadose Zone. Water, Air, & Soil Pollution 149, 327–337 (2003). https://doi.org/10.1023/A:1025693109248
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DOI: https://doi.org/10.1023/A:1025693109248