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Accumulation of soluble phenolic compounds in sunflower capitula correlates with resistance to Sclerotinia sclerotiorum

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Abstract

Disease symptoms and total soluble phenolics content have been analysed in four sunflower (Helianthus annuus L.)lines with different resistance levels(from highly susceptible to resistant) to head rot caused by Sclerotinia sclerotiorum (Lib.) de Bary. At the beginning of the flowering stage, capitula were inoculated by spraying with a water suspension of ascospores, and disease symptoms were evaluated from day 6 to day14 after inoculation. The most susceptible genotypes showed all their ovaries to be necrosed and abundant lesions in corollas, bracts and receptacle. In the resistant line, the ovary and corolla were only partially necrosed with no symptoms in the bracts or the receptacle. Total soluble phenolics were extracted and quantified from different parts of the capitulum in both inoculated and non-inoculated plants. The amount of phenolic compounds depended on the sunflower line, the time after inoculation, and the tissue. Higher constitutive and induced phenolic content as well as phenylalanine ammonia-lyase activity were present in the most resistant line, these differences correlated with the absence/presence of disease symptoms.

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Authors and Affiliations

  1. Grupo de Investigación Bioquímica Vegetal y Agrícola, Departamento de Bioquímica y Biología Molecular (ETSIAM), Universidad de Córdoba, Apdo 3048, 14080, Córdoba, España (EPP y JVJN)

    E. Prats & J.V. Jorrín

  2. Advanta Semillas S.A.I.C, Ruta 226, Km 60,5. C.C. 30, 7620 Balcarce, Provincia de Buenos Aires, Argentina

    M.E. Bazzalo & A. León

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  1. E. Prats
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  2. M.E. Bazzalo
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  3. A. León
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  4. J.V. Jorrín
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Prats, E., Bazzalo, M., León, A. et al. Accumulation of soluble phenolic compounds in sunflower capitula correlates with resistance to Sclerotinia sclerotiorum . Euphytica 132, 321–329 (2003). https://doi.org/10.1023/A:1025046723320

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  • DOI: https://doi.org/10.1023/A:1025046723320

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