Skip to main content
SpringerLink
Log in

Growth promotion of bean (Phaseolus vulgaris L.) by a polyamine-producing isolate of Streptomyces griseoluteus

  • Published:
Plant Growth Regulation Aims and scope Submit manuscript

Abstract

Of seventy-five actinomycetes isolated from a bean rhizosphere in the United Arab Emirates, an isolate of Streptomyces griseoluteus (WT) was found to be capable of producing relatively high levels of putrescine on decarboxylase agar medium and to produce putrescine, spermidine and spermine in liquid decarboxylase medium. In the glasshouse, the application of the WT strain to soil amended with arginine (as a precursor for putrescine) significantly (P < 0.05) promoted the growth of bean plants and increased the fresh and dry weights and lengths of roots and shoots, compared with control plants. Infestation of soil with the WT strain resulted in a significant (P < 0.05) increase in the levels of putrescine, spermidine and spermine, certain endogenous plant growth regulators (PGRs) (indole-acetic acid, and gibberellic acid), chlorophylls (a, b) and carotenoids with a concomitant reduction in the level of abscisic acid in bean plants, compared with control plants. A polyamine non-producing mutant strain (PNPM) obtained from the wild-type isolate (WT), however, failed to promote plant growth. There were no significant (P > 0.05) differences between the levels of polyamines, endogenous PGRs, chlorophylls (a, b), and carotenoids between plants that were not exposed to either of the strain (control) and those grown in soil with the PNPM strain. Both WT and PNPM strains were incapable of producing in vitro detectable levels of PGRs, indole-acetic acid, indole-pyruvic acid, gibberellic acid, isopentenyl adenine and zeatin in the culture filtrates. This study is the first to demonstrate the potential of a polyamine-producing actinomycete to promote plant growth. In addition, it is also the first published report of the production of polyamines by streptomycete actinomycetes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Ali A.G. and Lovatt C.J. 1995. Relationship of polyamines to lowtemperature stress-induced flowering of the Washington navel orange (Citrus sinensis L. Osbeck). J. Hortic. Sci. 70: 491–498.

    Google Scholar 

  • Arena M.E. and Manca de Nadra M.C. 2001. Biogenic amine production by Lactobacillus. J. Appl. Microbiol. 90: 158–162.

    Google Scholar 

  • Bagni N. and Tassoni A. 2001. Biosynthesis, oxidation and conjugation of aliphatic polyamines in higher plants. Amino acids 20: 301–317.

    Google Scholar 

  • Bais H.P., Sudha G.S. and Gokare A.R. 2000. Putrescine and silver nitrate influences shoot multiplication, in vitro flowering and endogenous titers of polyamines in Cichorium intybus L. cv. Lucknow local. J. Plant Growth Regul. 19: 238–248.

    Google Scholar 

  • Beranger-Novat N., Monin J. and Martin-Tanguy J. 1994. Polyamines and their biosynthetic enzymes in dormant em-bryos of the spindle tree (Euonymus europaeus L.) and in dormancy break obtained after treatment with gibberellic acid. Plant Sci. 102: 139–145.

    Google Scholar 

  • Besford R.T., Richardson C.M., Campos J.L. and Tiburcio A.F. 1993. Effect of polyamines on stabilization of molecular complexes of thylakoid membranes of osmotically stressed oat leaves. Planta 189: 201–206.

    Google Scholar 

  • Bover-Cid S., Hugas M., Izquierdo-Pulido M. and Vidal-Carou M.C. 2001. Amino acid decarboxylase activity of bacteria isolated from fermented pork sausages. Int. J. Food Microbiol. 66: 185–189.

    Google Scholar 

  • Cho S.C. 1983. Enhancement by putrescine of gibberellin-induced elongation in hypocotyls of lettuce seedlings. Plant Cell Physiol. 24: 305–308.

    Google Scholar 

  • Cross T. 1989. Growth and examination of actinomycete, some guidelines. In: Williams S.T., Sharpe M.E. and Holt J.G. (eds), Bergey's Manual of Systematic Bacteriology. Williams and Wilkins, Baltimore, MD, USA, pp. 2340–2343.

    Google Scholar 

  • Davies B.H. 1965. Analysis of carotenoid pigments. In: Goodwin T.W. (ed.), Chemistry and Biochemistry of Plant Pigments. Academic Press, London, UK, pp. 489–532.

    Google Scholar 

  • El-Tarabily K.A., Hardy G.E.St.J., Sivasithamparam K. and Kurtböke I.D. 1996. Microbiological differences between limed and unlimed soils and their relationship with cavity spot disease of carrots (Daucus carota L.) caused by Pythium coloratum in Western Australia. Plant Soil 183: 279–290.

    Google Scholar 

  • El-Tarabily K.A., Soliman M.H., Nassar A.H., Al-Hassani H.A., Sivasithamparam K., McKenna F. et al. 2000. Biological control of Sclerotinia minor using a chitinolytic bacterium and actinomycetes. Plant Pathol. 49: 573–583.

    Google Scholar 

  • Evans P.T. and Malmberg R.L. 1989. Do polyamines have roles in plant development? Annu. Rev. Plant Phys. Plant Mol. Biol. 40: 235–269.

    Google Scholar 

  • Flores H.E. and Galston A.W. 1982. Analysis of polyamines in higher plants by high performance liquid chromatography. Plant Physiol. 69: 701–706.

    Google Scholar 

  • Friedman R., Altman A. and Bachrach U. 1982. Polyamines and root formation in mung bean hypocotyl cuttings I. Effects of exogenous compounds and changes in endogenous polyamine content. Plant Physiol. 70: 844–848.

    Google Scholar 

  • Galston A.W. and Kaur-Sawhney R. 1990. Polyamines in plant physiology. Plant Physiol. 94: 406–410.

    Google Scholar 

  • Geneve R.L. and Kester S.T. 1991. Polyamines and adventitious root formation in the juvenile and mature phase of English ivy. J. Exp. Bot. 42: 71–75.

    Google Scholar 

  • Guinn G., Brummett D.L. and Beier R.C. 1986. Purification and measurement of abscisic acid and indole-acetic acid by high performance liquid chromatography. Plant Physiol. 81: 997–1002.

    Google Scholar 

  • Holden M. 1965. Chlorophylls. In: Goodwin T.W. (ed.), Chemistry and Biochemistry of Plant Pigments. Academic Press, London, UK, pp. 462–488.

    Google Scholar 

  • Jarvis B.C., Shannon P.R. and Yasmin S. 1983. Involvement of polyamines with adventitious root development in stem cuttings of mung bean. Plant Cell Physiol. 24: 677–683.

    Google Scholar 

  • JiZhong X., Haijiang C., BaoKun M. and WenCai Z. 2001. Effect of exogenous spermidine on the levels of endogenous hormones and polyamines in the flowers and fruitlets of red Fuji apple. Acta Hortic. Sin. 28: 206–210.

    Google Scholar 

  • Kakkar R.K. and Nagar P.K. 1996. Polyamines and senescence of maintenance foliage of tea, Camellia sinensis L. Biol. Plantarum 38: 153–157.

    Google Scholar 

  • Krizek D.T., Kramer G.F. and Mirecki R.M. 1997. Influence of UV-B radiation and putrescine on shoot and root growth of cucumber seedlings grown in nutrient solution. J. Plant Nutr. 20: 613–623.

    Google Scholar 

  • Lee M., Lee S. and Park K. 1997. Effects of spermine on ethylene biosynthesis in cut carnation (Dianthus caryophyllus L.) flowers during senescence. J. Plant Physiol. 151: 68–73.

    Google Scholar 

  • Locci R. 1989. Streptomycetes and related genera. In: Williams S.T., Sharpe M.E. and Holt J.G. (eds), Bergey's Manual of Systematic Bacteriology. Williams and Wilkins, Baltimore, MD, USA, pp. 2451–2508.

    Google Scholar 

  • Lynch J.M. 1985. Origin, nature and biological activity of aliphatic substances and growth hormones found in soil. In: Vaughan D. and Malcolm R.E. (eds), Soil Organic Matter and Biological Activity. Martinus Nijhoff/Dr. W. Junk Publishers, Dordrecht, The Netherlands, pp. 151–174.

    Google Scholar 

  • Martin-Tanguy J. 2001. Metabolism and function of polyamines in plants: recent development (new approaches). Plant Growth Regul. 34: 135–148.

    Google Scholar 

  • Miller J.H. 1972. Experiments in Molecular Genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA, pp. 121–185.

    Google Scholar 

  • Mo H. and Pua E.C. 2002. Up-regulation of arginine decarboxylase gene expression and accumulation of polyamines in mustard (Brassica juncea) in response to stress. Physiol. Plantarum 114: 439–449.

    Google Scholar 

  • Nag S., Saha K. and Choudhuri M.A. 2001. Role of auxins and polyamines in adventitious root formation in relation to changes in compounds involved in rotting. Journal Plant Growth Regul. 20: 182–194.

    Google Scholar 

  • Nassar A.H. 1997. Physiological responses to polyamine treatments in Pisum sativum L. Ph.D, Ain-Shams University, Cairo, Egypt.

    Google Scholar 

  • Nonomura H. and Hayakawa M. 1988. New methods for the selective isolation of soil actinomycetes. In: Okami Y., Beppu T. and Oegawara H. (eds), Biology of Actinomycetes. Japan Sci. Soc. Press, Tokyo, Japan, pp. 288–293.

    Google Scholar 

  • Rajasekaran L.R. and Blake T.J. 1998. Early growth invigoration of Jack pine seedlings by natural plant growth regulators. Trees-Struct. Funct. 12: 420–423.

    Google Scholar 

  • Redmond J.W. and Tseng A. 1979. High-pressure liquid chromatographic determination of putrescine, spermidine and spermine. J. Chromatogr. 170: 479–481.

    Google Scholar 

  • Sharma M.L. and Ali M. 1998. Polyamines as modulators of soybean productivity. J. Agron. Crop Sci. 181: 189–191.

    Google Scholar 

  • Shindy W.W. and Smith O.E. 1975. Identification of plant hormones from cotton ovules. Plant Physiol. 55: 550–554.

    Google Scholar 

  • Sinska I. 1988. Stimulation of dark germination of light-sensitive lettuce seeds by polyamines. Acta Physiol. Plant. 10: 11–16.

    Google Scholar 

  • Smith M.A. and Davies P.J. 1985. Separation and quantitation of polyamines in plant tissue by high performance liquid chromatography of their dansyl derivatives. Plant Physiol. 78: 89–91.

    Google Scholar 

  • ten Brink B., Damink C., Joosten H.M. and Huis in't Veld J.H. 1990. Occurrence and formation of biologically active amines in foods. Int. J. Food Microbiol. 11: 73–84.

    Google Scholar 

  • Tiburcio A.F., Campos J.L., Figueras X. and Besford R.T. 1993. Recent advances in the understanding of polyamine functions during plant development. Plant Growth Regul. 12: 331–340.

    Google Scholar 

  • Tien T.M., Gaskings M.H. and Hubbell D.H. 1979. Plant growth substances produced by Azospirillum brasilense and their effect on the growth of pearl millet (Pennisetum americanum L.). Appl. Environ. Microbiol. 37: 1016–1024.

    Google Scholar 

  • Todorov D., Alexieva V. and Karanov E. 1998. Effect of putrescine, 4-PU-30, and abscisic acid on maize plants grown under normal, drought and rewatering conditions. J. Plant Growth Regul. 17: 197–203.

    Google Scholar 

  • Upreti K.K. and Murti G.S. 1999. Effect of polyamines on the changes in endogenous hormones in pea under water stress conditions. Indian J. Plant Physiol. 4: 1–5.

    Google Scholar 

  • Velikov V., Yordanov I. and Edreva A. 2000. Oxidative stress and some antioxidant systems in acid rain-treated bean plants. Protective role of exogenous polyamines. Plant Sci. 151: 59–66.

    Google Scholar 

  • Wada N., Shinozaki M. and Iwamura H. 1994. Flower induction by polyamines and related compounds in seedlings of morning glory (Pharbitis nil cv. Kidachi). Plant Cell Physiol. 35: 469–472.

    Google Scholar 

  • Walker M.A., Roberts D.R., Shih C.Y. and Dumbroff E.B. 1985. A requirement for polyamines during the cell division phase of radicle emergence in seeds of Acer saccharum. Plant Cell Physiol. 26: 967–972.

    Google Scholar 

  • Walters D.R. 2000. Polyamines in plant-microbe interactions. Physiol. Mol. Plant Pathol. 57: 137–146.

    Google Scholar 

  • Wen-Quan S. 1989. Path analysis of relationship between the endogenous polyamines and lateral root initiation in apple seedlings. Acta Phytophysiol. Sin. 15: 388–392.

    Google Scholar 

  • Xu Y.C., Wang J., Shan L., Dong X. and Li M.M. 2001. Effect of exogenous polyamines on glycolate oxidase activity and active oxygen species accumulation in wheat seedlings under osmotic stress. Israel J. Plant Sci. 49: 173–178.

    Google Scholar 

  • Zheleva D., Tsonev T., Sergiev I. and Karanov E. 1994. Protective effect of exogenous polyamines against atrazine in pea plants. J. Plant Growth Regul. 13: 203–211.

    Google Scholar 

Download references

Author information

Author notes

  1. Amr H. Nassar & Khaled A. El-Tarabily

    Present address: Faculty of Science, Department of Biology, United Arab Emirates University, Al-Ain, 17551, United Arab Emirates

Authors and Affiliations

  1. Department of Botany and Microbiology, respectively, Faculty of Science, University of Ain Shams, Cairo, 11566, Egypt

    Amr H. Nassar & Khaled A. El-Tarabily

  2. Faculty of Natural and Agricultural Sciences, Department of Soil Science and Plant Nutrition, University of Western Australia, Crawley, 6009, Australia (e-mail

    Krishnapillai Sivasithamparam

Authors
  1. Amr H. Nassar
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Khaled A. El-Tarabily
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Krishnapillai Sivasithamparam
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Nassar, A.H., El-Tarabily, K.A. & Sivasithamparam, K. Growth promotion of bean (Phaseolus vulgaris L.) by a polyamine-producing isolate of Streptomyces griseoluteus . Plant Growth Regulation 40, 97–106 (2003). https://doi.org/10.1023/A:1024233303526

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1024233303526

Search

Navigation