Abstract
Escape of transgenes from genetically modified oilseed rape, Brassica napus, into wild radish, Raphanus raphanistrum, depends on sexual compatibility. The variation in prezygotic barriers of two different cultivars for interspecific hybridization with a population of wild radish was investigated by hand crossing and fluorescence microscopy of pistils. Significant differences were observed between oil seed rape cultivars in their ability to accept wild radish pollen germinating onto their stigma and the rate of fertilization of ovules. Some differences among the pollen donor plants were also detected. These results suggest that the rate of interspecific hybridization in the field would depend upon the oilseed rape cultivar and the genotype composition of the local wild populations. The implication of S-related genes, as revealed through identification by pistil tissue prints of class I and II S-types of SLG (S-Locus Glycoprotein) and SLR1 (S-Locus Related),and immuno-IEF, was not significant.
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References
Alexander, M.P., 1969. Differential staining of aborted and non aborted pollen. Stain Technol 44: 117–122.
Baranger, A., A.M. Chèvre, F. Eber & M. Renard, 1995. Effect of oilseed rape genotype on the spontaneous hybridization rate with a weedy species, an assessment of transgene dispersal. Theor Appl Genet 91: 956–963.
Cappadocia, M., P. Heizmann & C. Dumas, 1993. Tissue printing and its applications in self-incompatibility studies. Plant Mol Biol 23: 1079–1085.
Chadoeuf, R., Darmency, H., Maillet, J. & M. Renard, 1998. Survival of buried seeds interspecific hybrids between oilseed rape, hoary mustard and wild radish. Field Crops Res 58: 197–204.
Chèvre, A.M., F. Eber, H. Darmency, A. Fleury, H. Picault, J.C. Letanneur & M. Renard, 2000. Assessment of interspecific hybridization between transgenic oilseed rape and wild radish under agronomic conditions. Theor Appl Genet 100: 1233–1239.
Darmency, H., E. Lefol & A. Fleury, 1998. Spontaneous hybridizations between oilseed rape and wild radish. Mol Ecol 7: 1467–1473.
Dumas, C. & R.B. Knox, 1983. Callose and determination of pistil viability and incompatibility. Theor Appl Genet 67: 1–10.
Gaude, T., A. Friry, P. Heizmann, C. Mariac, M. Rougier, I. Fobis & C. Dumas, 1993. Expression of a self-incompatibility gene in a self-compatible line of Brassica oleracea. The Plant Cell 5: 75–86.
Guéritaine, G. & H. Darmency, 2001. Polymorphism for interspecific hybridization between oilseed rape (Brassica napus) and a population of wild radish (Raphanus raphanistrum). Sex Plant Reprod 14: 169–172.
Heizmann, P., D.T. Luu & C. Dumas, 2000. Stigma Adhesion in the Brassicaceae. Ann Botany 85: 23–27.
Karron, J.D., D.L. Marshall & D.M. Oliveras, 1990. Numbers of sporophytic self-incompatibility alleles in populations of wild radish. Theor Appl Genet 79: 457–460.
Kerlan, M.C., A.M. Chèvre, F. Eber, A. Baranger & M. Renard, 1992. Risk of assesment of outcrossing of transgenic rapeseed to related species: I. Interspecific hybrid production under optimal conditions with emphasis on pollinisation and fertilization. Euphytica 62: 145–153.
Lefol, E., A. Fleury & H. Darmency, 1996. Gene dispersal from transgenic crops II Hybridization between oilseed rape and the hoary mustard. Sex Plant Reprod 9: 189–196.
Lewis, D. & L.K. Crowe, 1958. Unilateral incompatibility in flowering plants. Heredity 12: 233–256.
Luu, D.T., P. Heizmann, C. Dumas, M. Trick & M. Cappadocia, 1997. Involvement of SLR1 genes in pollen adhesion to the stigmatic surface in Brassicaceae. Sex Plant Reprod 10: 227–235.
Luu, D.T., D. Marty-Mazars, M. Trick, C. Dumas & P. Heizmann, 1999. Pollen-Stigma adhesion in Brassica sp. involves SLG and SLR1 proteins. The Plant Cell 11: 251–262.
Martin, F.W., 1959. Staining and observing pollen tubes in the style by means of fluorescence. Stain Technol 34: 125–128.
McCubbin, A.G. & T.H. Kao, 2000. Molecular recognition and response in pollen and pistil interactions. Ann Rev Cell Dev Biol 16: 333–364.
Nasrallah, J.B. & M.E. Nasrallah, 1989. The molecular genetics of self-incompatibility in Brassica. Ann Rev Genet 23: 121–139.
Rieger, L.H., C. Preston & S.B. Powles, 1999. Risks of gene flow from transgenic herbicide-resistant canola (Brassica napus) to weedy relatives in southern Australian cropping systems Aust J Agri Res 50: 115–128.
Rieger, M.A., T.D. Potter, C. Preston & S.B. Powles, 2001. Hybridization between Brassica napus L. and Raphanus raphanistrum L. under agronomic field conditions. Theor Appl Genet 103: 555–560.
Systat 9, 1999. SYSTAT®, release 9 for Window, SPSS Inc., Chicago.
Trick, M. & R.B. Flavell, 1989. A homologous S genotype of Brassica oleracea express two S-like genes. Mol Gen Genet 218: 112–117.
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Guéritaine, G., Bonavent, J. & Darmency, H. Variation of prezygotic barriers in the interspecific hybridization between oilseed rape and wild radish. Euphytica 130, 349–353 (2003). https://doi.org/10.1023/A:1023021122374
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DOI: https://doi.org/10.1023/A:1023021122374