Skip to main content
Log in

Relationship between type and age of the inoculum cultures and betalains biosynthesis by Beta vulgaris hairy root culture

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

From a study of the relationship between the type and age of the inocula, and the growth and biosynthesis of betalains in a Beta vulgaris hairy root culture, the best results were achieved with a 14 d inoculum grown in submerged culture giving 42 mg betalains (16 mg betacyanins and 26 betaxanthins) and 1.5 g dry biomass in 40 ml medium.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Dixon RA (1985) Isolation and maintenance of callus and cell suspension cultures. In: Dixon RA, ed. Plant Cell Culture – A Practical Approach. Oxford: IRL Press, pp. 1–20. ISBN 0-947946-22-5.

    Google Scholar 

  • Hamill JD, Parr AJ, Robins RJ, Rhodes MJC (1986) Secondary product formation by cultures of Beta vulgaris and Nicotiana rustica transformed with Agrobacterium rhizogenes. Plant Cell Rep. 5: 111–114.

    Google Scholar 

  • Mukundan U, Bhide V, Dawda H (1999) Production of betalains by hairy root cultures of Beta vulgaris L. In: Fu TJ, Singh G, Curtis WR, eds. Plant Cell and Tissue Culture for the Production of Food Ingredients. New York: Kluwer Academic/Plenum Publ., pp 121–128. ISBN 0-306-46100-5.

    Google Scholar 

  • Nilsson T (1970) Studies into the pigments in beetroot (Beta vulgaris L. spp. vulgaris var. rubra L.). Luntbrukshogskolans Annaler. 36: 179–219.

    Google Scholar 

  • Pedreňo MA, Escribano J (2001) Correlation between antiradical activity and stability of betanine from Beta vulgaris L. roots under different pH, temperature and light conditions. J. Sci. Food Agric. 81: 627–631.

    Google Scholar 

  • Taya M, Mine K, Kino-Oka M, Tone S, Ichi T (1992) Production and release of pigments by culture of transformed hairy root of red beet. J. Ferment. Bioeng. 73: 31–36.

    Google Scholar 

  • Taya M, Yakura K, Kino-Oka M, Tone S (1994) Influence of medium constituents on enhancement of pigment production by batch culture of red beet hairy roots. J. Ferment. Bioeng. 77: 215–217.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Atanas Pavlov.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pavlov, A., Georgiev, V. & Kovatcheva, P. Relationship between type and age of the inoculum cultures and betalains biosynthesis by Beta vulgaris hairy root culture. Biotechnology Letters 25, 307–309 (2003). https://doi.org/10.1023/A:1022397317504

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1022397317504

Navigation