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Molecular tagging of a gene for resistance to brown planthopper in rice (Oryza sativa L.)

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Abstract

An introgression line derived from an interspecific cross between Oryzasativa and Oryza officinalis, IR54741-3-21-22 was found to beresistant to an Indian biotype of brown planthopper (BPH). Genetic analysisof 95 F3 progeny rows of a cross between the resistant lineIR54741-3-21-22 and a BPH susceptible line revealed that resistance wascontrolled by a single dominant gene. A comprehensive RAPD analysisusing 275 decamer primers revealed a low level of (7.1%) polymorphismbetween the parents.RAPD polymorphisms were either co-dominant (6.9%), dominant forresistant parental fragments (9.1%) or dominant for susceptible parentalfragments (11.6%). Of the 19 co-dominant markers, one primer,OPA16, amplified a resistant parental band in the resistant bulk and asusceptible parental band in the susceptible bulk by bulked segregantanalysis. RAPD analysis of individual F2 plants with the primerOPA16 showed marker-phenotype co-segregation for all, with only onerecombinant being identified. The linkage between the RAPD markerOPA16938 and the BPH resistance gene was 0.52 cM in couplingphase. The 938 bp RAPD amplicon was cloned and used as a probe on122 Cla I digested doubled haploid (DH) plants from aIR64xAzucena mapping population for RFLP inheritance analysis and wasmapped onto rice chromosome 11. The OPA16938 RAPD markercould be used in a cost effective way for marker-assisted selection of BPHresistant rice genotypes in rice breeding programs.

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References

  • Athwal, D.S., M.D. Pathak, E.H. Bacalangco & C.D. Pura, 1971. Genetics of resistance to brown planthopper and green leafhopper in Oryza sativa L. Crop Sci 11: 747-750.

    Google Scholar 

  • Beaumont, V.H., J. Mantet, T.R. Rocheford & J.M. Widholm, 1996. Comparison of RAPD and RFLP markers for mapping F2 generations in Maize (Zea mays). Theor Appl Genet 93: 606-612.

    Google Scholar 

  • Ford, R., E.C.K. Pang & P.W.J. Taylor, 1999. Genetics of resistance to ascochyta blight (Ascochyta lentis) of lentil and the identification of closely linked RAPD markers. Theor Appl Genet 98: 93-98.

    Google Scholar 

  • Huang, N., P. Arnold, T. Mew, G. Magpantay, S. McCouch, E. Guiderdoni, J. Xu, P. Subudhi, R. Angeles & G.S. Khush, 1997. RFLP mapping of isozymes, RAPD and QTLs for grain shape, brown planthopper resistance in a doubled haploid rice population. Mol Breed 1: 1-8.

    Google Scholar 

  • Ishii, T., D.S. Brar, D.S. Multani & G.S. Khush, 1994. Molecular tagging of genes for brown planthopper resistance and earliness introgressed from O. australiensis into cultivated rice, O. sativa. Genome 37: 217-221.

    Google Scholar 

  • Jena, K.K. & G.S. Khush, 1990. Introgression of genes from Oryza officinalis Well ex Watt. to cultivated rice, O. sativa L. Theor Appl Genet 80: 737-745.

    Google Scholar 

  • Jena, K.K. & G. Kochert, 1991. Restriction fragment length polymorphism analysis of CCDD genome species of the genus Oryza L. Plant Mol Bol 16: 831-839.

    Google Scholar 

  • Jena, K. K., G.S. Khush & G. Kochert, 1992. RFLP analysis of rice (Oryza sativa L.) introgression lines. Theor Appl Genet 84: 608-616.

    Google Scholar 

  • Jena, K.K., G.S. Khush & G. Kochert, 1994. Comparative RFLP mapping of a wild rice, Oryza officinalis, and cultivated rice, O. sativa. Genome 37: 382-389.

    Google Scholar 

  • Jena, K.K., I.C. Pasalu, K. Krishnaiah & G.S. Khush, 1998. A RAPD marker for the gene conferring resistance to Indian biotype of BPH. Rice Genet Newsl 15: 133-134.

    Google Scholar 

  • Jeon, Y.H., S.N. Ahn, H.C. Choi, T.R. Hahn & H.P Moon, 1999. Identification of a RAPD marker linked to a brown planthopper resistance gene in rice. Euphytica 107: 23-28.

    Google Scholar 

  • Kabir, M.A. & G.S. Khush, 1988. Genetic analysis of resistance of brown planthopper in rice, Oryza sativa L. Plant Breed 100: 54-58.

    Google Scholar 

  • Kalode, M.B. & T.S. Krishna, 1979. Varietal resistance to brown planthopper in India. In: Brown Planthopper: Threat to Rice Production in Asia. International Rice Research Institute, Manila, Philippines, pp. 369.

    Google Scholar 

  • Khush, G.S. & D.S. Brar, 1991. Genetics of resistance to insects in crop plants. Adv Agron 45: 223-274.

    Google Scholar 

  • Khush, G.S., D.S. Brar & B. Hardy, 2001. Rice genetics from Mendel to functional genomics. Rice Genet IV: 3-25.

    Google Scholar 

  • Kosambi, D.D., 1944. The estimation of map distances from recombination values. Ann Eugen 12: 172-175.

    Google Scholar 

  • Lakshminarayana, A. & G.S. Khush, 1997. New genes for resistance to brown planthopper in rice. Crop Sci 17: 96-100.

    Google Scholar 

  • Lander, E.S., P. Green, J. Abrahamson, A. Barlow, M.J. Daly, S.E. Lincoln & L. Newburg, 1987. MAPMAKER: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1: 174-181.

    Google Scholar 

  • Manninen, O.M., 2000. Associations between anther-culture response and molecular markers on chromosomes 2H, 3H and 4H of barley (Hordeum vulgare L.). Theor Appl Genet 100: 57-62.

    Google Scholar 

  • Martin, G.B., J.G.K. Williams & S.D. Tanksley, 1991. Rapid identification of markers linked to a Pseudomonas resistance gene in tomato by using random primers and near isogenic lines. Proc Natl Acad Sci USA 88: 2336-2340.

    Google Scholar 

  • McCouch, S.R., X. Chen, O. Panaud, S. Temnykh, Y. Xu, Y.G. Cho, N. Huang, T. Ishii & M. Blair, 1997. Microsatellite marker development, mapping and applications in rice genetics and breeding. Plant Mol Biol 35: 89-99.

    Google Scholar 

  • Mei, M., C. Zhuang, R. Wan, J. Wu, W. Hu & G. Kochert, 1996. Genetic analysis and tagging of gene for brown planthopper resistance in indica rice. In: G.S. Khush (Ed.), Rice Genetics III, pp. 590-595. International Rice Research Institute, Manila, Philippines.

    Google Scholar 

  • Michelmore, R.W., I. Paran & R.V. Kesseli, 1991. Identification of markers linked to disease resistance genes by bulked segregant analysis: A rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88: 9828-9832.

    Google Scholar 

  • Mohan, M., S. Nair, A. Bhagwat, T.G. Krishna, M. Yano, C.R. Bhatia & T. Sasaki, 1997. Genome mapping, molecular markers and marker-assisted selection in crop plants. Mol Breed 3: 87-103.

    Google Scholar 

  • Nair, S., J.S. Bentur, U. PrasadaRao & M. Mohan, 1995. DNA markers tightly linked to a gall midge resistance gene (Gm2) are potentially useful for marker-aided selection in rice breeding. Theor Appl Genet 91: 68-73.

    Google Scholar 

  • Shimano, T., T. Inoue, B.A. Antonio, K. Iliromi, A. Shomura, S.Y. Lin, Y. Kuboki, Y. Nagamura, N. Kurata, M. Yano & T. Sasaki, 1995. Extensive conservation in linkage alignment of RFLP markers between rice chromosome 11 and 12. Plant Genome 3: 75.

    Google Scholar 

  • Tanksley, S.D., N.D. Young, A.H. Paterson & M.W. Bonierbale, 1989. RFLP mapping in plant breeding: new tools for an old science. Bio / Technology 7: 257-264.

    Google Scholar 

  • Urrea, C.A., P.N. Miklas, J.S. Beaver & R.H. Riley, 1996. A codominant randomly amplified polymorphic DNA (RAPD) marker useful for indirect selection of BGMV resistance in common bean. J Am Soc Hort Sci 121: 1035-1039.

    Google Scholar 

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Authors and Affiliations

  1. Biotechnology Centre, Mahyco Research Foundation, 8-2-703, AG Heights, Road No. 12, Banjara Hills, Hyderabad, 500 034, India

    K.K. Jena & Y. Varalaxmi

  2. Plant Breeding, Genetics and Biochemistry Division, International Rice Research Institute, DAPO Box 7777, Metro Manila, Philippines

    K.K. Jena & G.S. Khush

  3. Rajendranagar, Hyderabad, 500 030, India

    I.C. Pasalu (Directorate of Rice Research), Y.K. Rao & K. Krishnaiah

  4. Department of Botany, University of Georgia, Athens, GA, 30602, U.S.A

    G. Kochert

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  1. K.K. Jena
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  2. I.C. Pasalu
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  3. Y.K. Rao
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  4. Y. Varalaxmi
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  5. K. Krishnaiah
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  6. G.S. Khush
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  7. G. Kochert
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Jena, K., Pasalu, I., Rao, Y. et al. Molecular tagging of a gene for resistance to brown planthopper in rice (Oryza sativa L.). Euphytica 129, 81–88 (2003). https://doi.org/10.1023/A:1021590025240

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