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Cultivar identification and genetic relationship among selected breeding lines and cultivars in chickpea (Cicer arietinum L.)

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Abstract

Twenty two RAPD and 22 ISSR markers were evaluated for their potential use in determination of genetic relationships in chickpea (Cicer arietinum L.) cultivars and breeding lines. We were able to identify six chickpea cultivars/breeding lines by cultivar-specific markers. All of the cultivars tested displayed a different phenotype generated either by the RAPD or ISSR primers. Though ISSR primers generated less markers than RAPD primers, the ISSR primers produced higher levels of polymorphism (% of polymorphic markers per primer) than RAPD primers. A high level of within cultivar homogeneity was observed in chickpea. Cultivars/breeding lines originating from a common genetic background showed closer genetic relationship. Chickpea lines with similar seed type(kabuli or desi) had a tendency to cluster together.

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References

  • Ahmed, F., 1999. Random amplified polymorphic DNA (RAPD) analysis reveals genetic relationships among the annual Cicer species. Theor Appl Genet 98: 657–663.

    Article  Google Scholar 

  • Ahmed, F., P.M. Gaur & A.E. Slinkard, 1992. Isozyme polymorphism and phylogenetic interpretation in the genus Cicer L. Theor Appl Genet 83: 620–627.

    Google Scholar 

  • Akkaya, M., A.A. Bhagwat & P.B. Cregan, 1992. Length polymorphisms of simple sequence repeat DNA in soybean. Genetics 132: 1131–1139.

    PubMed  CAS  Google Scholar 

  • Chan, K.F. & M. Susan, 1997. Genetic diversity and relationships detected by isozyme and RAPD analysis of crop and wild species of Amaranthus. Theor Appl Genet 95: 865–873.

    Article  CAS  Google Scholar 

  • Charters, Y.M., A. Robertson, M.J. Wilkinson & G. Ramsay, 1996. PCR analysis of oilseed rape cultivars (Brassica napus L. ssp. oleifera) using 5'-anchored sequence repeat (SSR) primers. Theor Appl Genet 92: 442–447.

    Article  CAS  Google Scholar 

  • Fang, D.Q. & M.L. Roose, 1997. Identification of closely related citrus cultivars with inter-simple sequence repeat markers. Theor Appl Genet 95: 408–417.

    Article  CAS  Google Scholar 

  • Gaur, P.M. & A.E. Slinkard, 1990. Inheritance and linkage of isozyme coding genes in chickpea. J Hered 81: 455–461.

    CAS  Google Scholar 

  • Kaga, A., M., Ohnishi, T. Ishii & O. Kamijima, 1996. A genetic linkage map of azuki bean constructed with molecular and morphological markers using an interspecific population (Vigna angularis X V. nakashimae). Theor Appl Genet 93: 658–663.

    Article  CAS  Google Scholar 

  • Kantely, R.V., X. Zeng, J.L. Bennetzen & B.E. Zehr, 1995. Assessment of genetic diversity in dent and popcorn (Zea mays L.) inbred lines using inter-simple sequence repeat (ISSR) amplification. Mol Breed 1: 365–373.

    Article  Google Scholar 

  • Kazan, K. & F.J. Muehlbauer, 1991. Allozyme variation and phylogeny in annual species of Cicer (Leguminosae). Plant Syst Evol 175: 11–21.

    Article  CAS  Google Scholar 

  • Lorenz, M, A. Weihe & T. Börner, 1997. Cloning and sequencing of RAPD fragments amplified from mitochondrial DNA of malesterile and male-fertile cytoplasm of sugar beet (Beta vulgaris L.). Theor Appl Genet 94: 273–278.

    Article  CAS  Google Scholar 

  • Micheli, M.R. & R. Bova, 1997. Fingerprinting Methods based on Arbitrarily Primed PCR. Springer, Berlin Heidelberg, New York.

    Google Scholar 

  • Micheli, M.R., R. Bova, E. Pascale & E. D'Ambrosio, 1994. Reproducible DNA fingerprinting with the random amplified polymorphic DNA (RAPD) method. Nucleic Acids Res 22: 1921–1922.

    PubMed  CAS  Google Scholar 

  • Morgante, M. & A.M. Olivieri, 1993. PCR-amplified microsatellites as markers in plant genetics. Plant J 3: 175–182.

    Article  PubMed  CAS  Google Scholar 

  • Murray, M.G. & W.F. Thompson, 1980. Rapid isolation of highmolecular-weight plant DNA. Nucleic Acid Res 8: 4321–4325.

    PubMed  CAS  Google Scholar 

  • Nei, M., 1978. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89: 583–590.

    Google Scholar 

  • Pa+akinskienë, I, C.M. Griffiths, A.J.E. Bettany, V. Paplauskien & M.W. Humphreys, 2000. Anchored simple-sequence repeats as primers to generate species specific DNA markers in Lolium and Festuca grasses. Theor Appl Genet 100: 384–390.

    Article  Google Scholar 

  • Paul, S., F.N. Wachira, W. Powell & R. Waugh, 1997. Diversity and genetic differentiation among populations of Indian and Kenyan tea (Camellia sinensis (L.) O. Kuntze) revealed by AFLP markers. Theor Appl Genet 94: 255–263.

    Article  CAS  Google Scholar 

  • Ratnaparkhe, M.B., D.K. Santra, A. Tullu & F.J. Muehlbauer, 1998. Inheritance of inter-simple-sequence-repeat polymorphisms and linkage with a fusarium gene in chickpea. Theor Appl Genet 96: 348–353.

    Article  CAS  Google Scholar 

  • Sant, V.J., A.G. Patankar, N.D. Sarode, L.B. Mhase, M.N. Sainani, R.B. Deshmukh, P.K. Ranjekar & V.S. Gupta, 1999 Potential of DNA markers in detecting divergence and in analysing heterosis in Indian elite chickpea cultivars. Theor Appl Genet 98: 1217–1225.

    Article  CAS  Google Scholar 

  • Sharma, S.K., M.R. Knox & T.H.N. Ellis, 1996. AFLP analysis of the diversity and phylogeny of Lens and its comparison with RAPD analysis. Theor Appl Genet 93: 751–758.

    Article  CAS  Google Scholar 

  • Sharma, P.C., P. Winter, T. Burger, B. Huttle, F. Weigand, K. Weising & G. Kahl, 1995. Abundance and polymorphism of di-, triand tetra-nucleotide tandem repeats in chickpea (Cicer arietinum L.). Theor Appl Genet 90: 90–96.

    Article  CAS  Google Scholar 

  • Simon, C.J. & F.J. Muehlbauer, 1997. Construction of a chickpea linkage map and its comparision maps of pea and lentil. J Hered 88: 115–119.

    CAS  Google Scholar 

  • Sneath, P.H.A. & R.R. Sokal, 1973. Numerical Taxonomy. Freeman, San Fransisco, CA.

    Google Scholar 

  • Sonnante, G., A. Marangi, D. Pignone & G. Venora, 1997. Using RAPD markers to investigate genetic variation in chickpea [Cicer arietinum L. - random amplified polymorphic DNA]. J Genet Breed 51: 303–307.

    CAS  Google Scholar 

  • Van Rheenen, H.A, 1992. Biotechnology and chickpea breeding. Int Chickpea Newsl 26: 14–17.

    Google Scholar 

  • Wang, Z., J.L. Weber, G. Zhong & S.D. Tanksley, 1994. Survey of plant short tandem DNA repeats. Theor Appl Genet 88: 1–6.

    CAS  Google Scholar 

  • Waycott, W. & S.B. Fort, 1994. Differentiation of nearly identical germplasm accessions by a combination of molecular and morphologic analyses. Genome 37: 577–583.

    CAS  Google Scholar 

  • Weising, K., D. Kaemmer, F. Weigand, J.T. Epplent & G. Kahl, 1992. Oligonucleotide fingerprinting reveals various probedependent levels of informativeness in chickpea (Cicer arietinum). Genome 35: 436–442.

    CAS  Google Scholar 

  • Weising, K., H. Nybom, W. Meyer & K. Wolff, 1994. DNA Fingerprinting in Plants and Fungi. CRC Press, Florida.

    Google Scholar 

  • Williams, J.G.K., A. Kubelik, K.J. Livak, J.A. Rafalski & S.V. Tingey, 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucl Acids Res 18: 6531–6535.

    PubMed  CAS  Google Scholar 

  • Wolff, K., E. Zietkiewicz & H. Hofstra, 1995. Identification of chrysanthemum cultivars and stability of DNA fingerprint patterns. Theor Appl Genet 91: 439–447.

    Article  CAS  Google Scholar 

  • Yeh, F.C. & T.J.B. Boyle, 1997. Population genetic analysis of codominant and dominant markers and quantitative traits. Belg J Botany 129: 157.

    Google Scholar 

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Authors and Affiliations

  1. Greenhouse and Processing Crops Research Centre, Agriculture and Agri-Food Canada, Harrow, Ontario, N0R 1G0, Canada

    M.A. Chowdhury

  2. Crop Development Centre, 51 Campus Drive, Saskatoon, Saskatchewan, S7N 5A8, Canada

    Bert Vandenberg & T. Warkentin

Authors
  1. M.A. Chowdhury
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  2. Bert Vandenberg
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  3. T. Warkentin
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Chowdhury, M., Vandenberg, B. & Warkentin, T. Cultivar identification and genetic relationship among selected breeding lines and cultivars in chickpea (Cicer arietinum L.). Euphytica 127, 317–325 (2002). https://doi.org/10.1023/A:1020366819075

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  • DOI: https://doi.org/10.1023/A:1020366819075

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