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Plant regeneration from interspecific hybrid and backcross progeny of Helianthus eggertii × Helianthus annuus

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Abstract

An efficient protocol for plant regeneration from leaves of the interspecific hybrid Helianthus eggertii Small. × Helianthus annuus L. was developed. The regeneration capacity of the first backcross progeny is reported. Leaves from the F1 interspecific hybrid were cultured on Murashige and Skoog basal media (MS) supplemented with α-naphthalenacetic acid (NAA), N 6-benzyladenine (BA), AgNO3, KNO3, casein hydrolysate and adenine sulfate. Embryo-like structures and/or shoots regeneration were observed on most of the tested media. The best results were obtained on media with a higher concentration of cytokinin (8.8 μM BA) and lower concentration of auxin (1.08 μM NAA). The addition of casein hydrolysate in the media increased the regeneration efficiency. Plant regeneration was achieved via somatic embryogenesis and direct organogenesis. The regeneration potential of leaf, stem and root explants of eighteen first backcross lines was studied. Most of the tested lines were highly regenerable and some of them had DNA content closely related to that of Helianthus annuus L.

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References

  • Alibert G, Christel A-Ch & Burrus M (1994) Sunflower tissue and cell culture and their use in biotechnology. Plant Physiol. Biochem. 32: 31–44

    Google Scholar 

  • Bohorova N, Atanassov A & Georgieva-Todorova J (1985) In vitro organogenesis, androgenesis and embryo culture, in the genus Helianthus L. Z. Pflanzenzuchtg. 95: 35–44

    Google Scholar 

  • Carter JF (1978) Sunflower Science and Technology. American Society of Agronomy, Madison,Wisconsin

    Google Scholar 

  • Chraibi KM, Latche A, Rousten JP & Fallot J (1991) Stimulation of shoot regeneration from cotyledons of Helianthus annuus L. by the ethylene inhibitors, silver and cobalt. Plant Cell Rep. 10: 204–207

    Google Scholar 

  • Chraibi BKM, Castelle JC, Latche A, Roustan JP & Fallot J (1992) Enhancement of shoot regeneration by liquid medium culture from mature cotyledons of sunflower (Helianthus annuus L.). Plant Cell Rep. 10: 617–620

    Google Scholar 

  • Christov M (1996) Characterisation of wild Helianthus species as sources of new features for sunflower breeding. In: Caligari PDS & Hind DJN (eds) Composite: Biology & Utilisation. Proceedings of the International Copmpositae Conference, Kew, 1994. (Hind DJF, editor-in-chief), 2: 547–570. Royal Botanical Gardens, Kew

    Google Scholar 

  • De Laat AMM, Gohde W & Vogelzang MJDC (1987) Determination of ploidy of single plants and plant population by flowcytometry. Plant Breed. 99: 303–307

    Google Scholar 

  • Deglene L, Lesignes Ph, Alibert G & Sarrafi A (1997) Genetic control of organogenesis in cotyledons of sunflower (Helianthus annuus). Plant Cell Tiss. Org. Cult. 48: 127–130

    Google Scholar 

  • Espinasse A & Lay C (1989) Shoot regeneration of callus derived from globular to torpedo embryos from 59 sunflower genotypes. Crop. Sci. 29: 201–205

    Google Scholar 

  • Espinasse A, Lay J & Volin J (1989) Effect of growth regulator concentration and explant size on shoot from callus derived from zygotic embryos of sunflower (Helianthus annuus L.). Plant Cell Tiss. Org. Cult. 17: 171–181

    Google Scholar 

  • Finer JJ (1987) Direct somatic embryogenesis and plant regeneration from immature embryos of hybrid sunflower (Helianthus annuus L.). Plant Cell Rep. 6: 372–374

    Google Scholar 

  • Freyssinet M & Freyssinet G (1988) Fertile plant regeneration from sunflower (Helianthus annuus L.) immature embryos. Plant Sci. 56: 177–181

    Google Scholar 

  • Gohde W, Schumann J, Buchner Th, Otto F & Barlogie B (1979) Pulse cytophotometry – application in tumour cell biology and clinical oncology. In: Melamend MR, Mullaney PF & Mendelson ML (eds) Flow Cytometry and Sorting. (p 599) John Wiley and Sons, New York

    Google Scholar 

  • Jeannin G & Hahne G (1991) Donor plant growth conditions and regeneration of fertile plants from somatic embryos induced on immature zygotic embryos of sunflower (Helianthus annuus L.). Plant Breeding 107: 280–287

    Google Scholar 

  • Paterson KE (1984) Shoot tip culture of Helianthus annuus -flowering and development of adventitious and multiple shoots. Am. J. Bot. 71: 925–931

    Google Scholar 

  • Laparra H, Stoeva P, Ivanov P & Hahne G (1997) Plant regeneration from different explants in Helianthus smithii Heiser. Plant Cell Rep. 16: 692–695

    Google Scholar 

  • Lupi MS, Bennici A, Locci F & Gennai D (1987) Plantlets formation from callus and shoot tip culture of Helianthus annuus L. Plant Cell Tiss. Org Cult. 11: 47–55

    Google Scholar 

  • Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol. Plant. 15: 473–497

    Google Scholar 

  • Paterson K E (1984) Shoot tip culture of Helianthus annuus -Flowering and development of adventitious and multiple shoots. Am. J. Bot. 71: 925–931

    Google Scholar 

  • Paterson KE & Everett NP (1985) Regeneration of Helianthus annuus inbred plants from callus. Plant Sci. 42: 125–132.

    Google Scholar 

  • Pelissier B, Bouchefra O, Pepin R & Freyssinet G (1990) Production of isolated somatic embryos from sunflower thin cell layers. Plant Cell Rep. 1990

  • Pouzet A & Delplancke D (2000) Evolution compare et de la cometitivite du tournesol dans differentes aries de production. Proc. of 15th Intertnational Sunflower Conference, France, 12-15 June, PL.A-1–PL.A-10

  • Power CJ (1987) Organogenesis from Helianthus annuus inbred lines and hybrids from the cotyledons of zygotic embryos. Am. J. Bot. 74: 497–503

    Google Scholar 

  • Prado E & Berville A (1990) Induction of somatic embryo development by liquid culture in sunflower (Helianthus annuus L.). Plant Sci. 67: 73–82

    Google Scholar 

  • Pugliesi C, Magale P, Cecconi F & Baroncelli S (1993) Organogenesis and embryogenesis in Helianthus tuberosus and in the interspecific hybrid Helianthus annuus × Helianthus tuberosus. Plant Cell Tiss. Org. Cult. 33: 187–193

    Google Scholar 

  • Pugliesi C, Fambribi M & Cavallini A (2000) Transgenic Sunflower (Helianthus annuus). In: Bajaj YPS (ed) Biotechnology in Agriculture and Forestry (46: 303–324). Transgenic crops I. Springer, Berlin, Heidelberg

    Google Scholar 

  • Sarrafi A, Flores-Berios E, Bolandi A, Deglen L, Berville A, Souvre A, Sereys H, Griveau Y & Alibert G (1999) Genetic control of organogenesis and somatic embryogenesis in sunflower. HELIA (Annex 2) 22: 186

    Google Scholar 

  • Steinrucken G & Grunenwaldt (1984) In vitro Regeneration bei Helianthus tuberosus L. Gartenbauwissenschaft 49: 266–269

    Google Scholar 

  • Sugatha M & Prabakaran A J (1997) Propagation and maintenance of wild sunflower in vitro. HELIA 20: 107–114

    Google Scholar 

  • Tomson T, Zimmerman D & Roters C (1981) Wild Helianthus as a genetic resource. Field Crops Res. 4: 333–343

    Google Scholar 

  • Wilcox McCann A, Cooly G & Van Dresser J (1988) A system for routine plantlet regeneration of sunflower (Helianthus annuus L.) from immature embryo-derived callus. Plant Cell Tiss. Org. Cult. 14: 103–110

    Google Scholar 

  • Wirtzens B, Scowcroft WR, Downes RW & Larkin PJ (1988) Tissue culture and plant regeneration from sunflower (Helianthus annuus) and interspecific hybrids (H. tuberosus × H. annuus). Plant Cell Tiss. Org. Cult. 13: 61–76

    Google Scholar 

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Authors and Affiliations

  1. AgroBioInstitute, 2232, Kostinbrod-2, Bulgaria

    Y. Yordanov, E. Yordanova & A. Atanassov

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  1. Y. Yordanov
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  2. E. Yordanova
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  3. A. Atanassov
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Correspondence to Y. Yordanov.

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Yordanov, Y., Yordanova, E. & Atanassov, A. Plant regeneration from interspecific hybrid and backcross progeny of Helianthus eggertii × Helianthus annuus . Plant Cell, Tissue and Organ Culture 71, 7–14 (2002). https://doi.org/10.1023/A:1016510109911

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