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Determination of β-Endorphin and Fragments Thereof in Human Plasma Using High-Performance Liquid Chromatography and a Multiple Radioimmunoassay System

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Abstract

A method for the determination of β-endorphin and β-endorphin fragments in human plasma was developed. β-Endorphin-related peptides were extracted from plasma using octadecasilyl-silica cartridges. Extracts were subjected to re versed-phase high-performance liquid chromatography (HPLC). Extracts as well as HPLC column eluates were assayed using a multiple radioimmunoassay system; several antibodies directed against various distinct regions of the β-endorphin molecule were employed. Using this method, evidence for the presence of multiple β-endorphin fragments in the plasma of healthy young volunteers (under normal conditions) was obtained.

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Authors and Affiliations

  1. Rudolf Buchheim-Institut für Pharmakologie der Justus Liebig-Universität, Frankfurter Str. 107, D-6300, Gieβen, FRG

    Klaus Wiedemann & Hansjörg Teschemacher

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  1. Klaus Wiedemann
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  2. Hansjörg Teschemacher
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Wiedemann, K., Teschemacher, H. Determination of β-Endorphin and Fragments Thereof in Human Plasma Using High-Performance Liquid Chromatography and a Multiple Radioimmunoassay System. Pharm Res 3, 142–149 (1986). https://doi.org/10.1023/A:1016357806879

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  • DOI: https://doi.org/10.1023/A:1016357806879

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