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Assessment of genetic variability in grapefruits (Citrus paradisi Macf.) and pummelos (C. maxima (Burm.) Merr.) using RAPD and SSR markers

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Abstract

The genetic variability of 38 grapefruit (Citrus paradisi Macf.) and three pummelos (C. maxima (Burm.) Merr..) accessions was evaluated using RAPD, and single sequence repeat (SSR) analyses. Approximately49% of the 198 RAPD were polymorphic, and 4.6 alleles per SSR loci were identified. PIC values changed from 0.093 to 0.450. A UPGMA phenetic tree was constructed and two main grapefruit groups were identified. The grapefruit accessions `do Cabo' and `Siamesa-Filipinas'clustered very close to the pummelos in Group A. The Group B consisted of three sub-groups, which comprised all of the other grapefruit accessions. The majority of grapefruit accessions showed a narrow genetic base suggesting that the observed morphological polymorphism within the group must be associated with somatic mutations, which were not detected by these molecular markers.

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Authors and Affiliations

  1. Departamento de Biologia, Universidade Estadual de Maringá, CEP 87020-900, Maringá-PR, Brazil

    M.J. Corazza-Nunes

  2. Centro de Citricultura Sylvio Moreira/IAC, Cordeirópolis-SP, Brazil

    M.A. Machado, M. Cristofani & M.L.P.N. Targon

  3. Departamento de Agronomia, Universidade Estadual de Maringá, Maringá-PR, Brazil

    W.M.C. Nunes

Authors
  1. M.J. Corazza-Nunes
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  2. M.A. Machado
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  3. W.M.C. Nunes
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  4. M. Cristofani
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  5. M.L.P.N. Targon
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Corazza-Nunes, M., Machado, M., Nunes, W. et al. Assessment of genetic variability in grapefruits (Citrus paradisi Macf.) and pummelos (C. maxima (Burm.) Merr.) using RAPD and SSR markers. Euphytica 126, 169–176 (2002). https://doi.org/10.1023/A:1016332030738

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  • DOI: https://doi.org/10.1023/A:1016332030738

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