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The home stretch, a first analysis of the nearly completed genome of Rhodobacter sphaeroides 2.4.1

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Abstract

Rhodobacter sphaeroides 2.4.1 is an α-3 purple nonsulfur eubacterium with an extensive metabolic repertoire. Under anaerobic conditions, it is able to grow by photosynthesis, respiration and fermentation. Photosynthesis may be photoheterotrophic using organic compounds as both a carbon and a reducing source, or photoautotrophic using carbon dioxide as the sole carbon source and hydrogen as the source of reducing power. In addition, R. sphaeroides can grow both chemoheterotrophically and chemoautotrophically. The structural components of this metabolically diverse organism and their modes of integrated regulation are encoded by a genome of ∼4.5 Mb in size. The genome comprises two chromosomes CI and CII (2.9 and 0.9 Mb, respectively) and five other replicons. Sequencing of the genome has been carried out by two groups, the Joint Genome Institute, which carried out shotgun-sequencing of the entire genome and The University of Texas-Houston Medical School, which carried out a targeted sequencing strategy of CII. Here we describe our current understanding of the genome when data from both of these groups are combined. Previous work had suggested that the two chromosomes are equal partners sharing responsibilities for fundamental cellular processes. This view has been reinforced by our preliminary analysis of the virtually completed genome sequence. We also have some evidence to suggest that two of the plasmids, pRS241a and pRS241b encode chromosomal type functions and their role may be more than that of accessory elements, perhaps representing replicons in a transition state.

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Authors and Affiliations

  1. Department of Microbiology and Molecular Genetics, University of Texas-Houston Medical School, 6431 Fannin St., Houston, TX 77030, USA

    Chris Mackenzie & Madhusudan Choudhary

  2. Life Sciences Division, 1060 Commerce Park, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA

    Frank W. Larimer

  3. DOE Joint Genome Institute, 2800 Mitchell Drive, B400, Walnut Creek, CA, 94598, USA

    Paul F. Predki & Stephanie Stilwagen

  4. Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK

    Judith P. Armitage

  5. Department of Biological Sciences, University of Wisconsin-Parkside, Greenquist Hall, Kenosha, WI, 53141, USA

    Robert D. Barber

  6. Bacteriology Department, University of Wisconsin-Madison, 1550 Linden Drive, Madison, WI, 53706, USA

    Timothy J. Donohue & Jack E. Newman

  7. Department of Biochemistry, University of Mississippi Medical Center, 2500 N. State St., Jackson, MS, 39216, USA

    Jonathan P. Hosler

  8. Department of Microbiology, Wing Hall, Cornell University, Ithaca, NY 14853-8101, USA

    James P. Shapleigh

  9. Institute of Genetics, University of Nottingham, QMC, Nottingham, NG7 2UH, UK

    R. Elizabeth Sockett

  10. Department of Biological Sciences, Oakland University, Rochester, MI, 48309, USA

    Jill Zeilstra-Ryalls

  11. Department of Microbiology and Molecular Genetics, University of Texas-Houston Medical School, 6431 Fannin St., Houston, TX, 77030, USA

    Samuel Kaplan

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  1. Chris Mackenzie
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  2. Madhusudan Choudhary
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  7. Robert D. Barber
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  14. Samuel Kaplan
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Correspondence to Samuel Kaplan.

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Mackenzie, C., Choudhary, M., Larimer, F.W. et al. The home stretch, a first analysis of the nearly completed genome of Rhodobacter sphaeroides 2.4.1. Photosynthesis Research 70, 19–41 (2001). https://doi.org/10.1023/A:1013831823701

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