Abstract
Studies were carried out for the production of aroma compounds by Kluyveromyces marxianus grown on cassava bagasse in solid state fermentation using packed bed reactors, testing two different aeration rates. Respirometric analysis was used to follow the growth of the culture. Headspace analysis of the culture by gas chromatography showed the production of 11 compounds, out of which nine were identified. Ethyl acetate, ethanol and acetaldehyde were the major compounds produced. Lower aeration rate (0.06l h−1 g−1 of initial dry matter) increased total volatile (TV) production and the rate of production was also increased at this aeration rate. Using an aeration rate of 0.06l h−1 g−1 maximum TV concentrations were reached at 24 h and at 40 h with 0.12l h−1 g−1.
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References
Berger, R.G. 1995 Aroma Biotechnology, Berlin: Springer-Verlag. ISBN 3-54058606-7.
Bramorski, A., Christen, P., Ramirez, M., Soccol, C.R. & Revah, S. 1998 Production of volatile compounds by the edible fungus Rhizopus oryzae during solid state cultivation on tropical agroindustrial substrates. Biotechnology Letters 20, 359-362.
Christen, P., Meza, J.C. & Revah, S. 1997 Fruity aroma production in solid state fermentation by Ceratocystis fimbriata: influence of the substrate type and the presence of precursors. Mycological Research 101, 911-919.
Christen, P., Bramorski, A., Revah, S. & Soccol, C.R. 2000 Characterization of volatile compounds produced by Rhizopus strains grown on agro-industrial solid wastes. Bioresource Technology 71, 211-215.
Fabre, C.E., Duviau, V.J., Blanc, P.J. & Goma, G. 1995 Identification of volatile flavour compounds obtained in culture of Kluyveromyces marxianus. Biotechnology Letters 17, 1207-1212.
Gervais, P. & Sarrette, M. 1990 Influence of age of mycelia and water activity on aroma production by Trichoderma viride. Journal of Fermentation and Bioengineering 69, 46-50.
Humphrey, M., Pearce, S. & Skill, B. 1990 Biotransformation of coconut fat to methyl ketones. A commercial scale solid state fermentation. In Abstracts of the Symposium on Bioinformation of Flavours, London.
Ito, K., Yoshida, K., Ishikawa, T. & Kobayashi, S. 1990 Volatile compounds produced by fungus Aspergillus oryzae in rice koji and their changes during cultivation. Journal of Fermentation and Bioengineering 70, 169-172.
Janssens, L., de Pooter, H.L., Demey, L., Vandamme, E.J. & Schamp, N.M. 1988 Fruity flavours by fermentation. Medical Faculteit Landbouww. Rijksuniversiteit Gent. 53, 2071-2077.
Janssens, L., de Pooter, H.L., Schamp, N.M. & Vandamme, E.J. 1992 Production of flavours by micro-organisms. Process Biochemistry 27, 195-215.
Jiang, J. 1995 Changes in volatile composition of Kluyveromyces lactis broth during fermentation. In Food Flavours, Generation Analysis and Process Influence, ed. Charalambous G. pp. 1073-1086. Elsevier Science B.V., New York. ISBN 0-44482013-2.
Medeiros, A.B.P., Pandey, A., Freitas, R.J.S., Christen, P. & Soccol, C.R. 2000 Optimization of the production of aroma compounds by Kluyveromyces marxianus in solid state fermentation using factorial design and response surface methodology. Biochemical Engineering Journal 6, 33-39.
Meza, J.C., Christen, P. & Revah, S. 1998 Effect of added amino acids on the production of a fruity aroma by Ceratocystis fimbriata. Science de e Alimentation 18, 627-636.
Pandey, A. 1991 Aspects of design of fermenter in solid state fermentation. Process Biochemistry 26, 355-361.
Pandey, A. & Soccol, C.R. 1998 Bioconversion of biomass: a case study of lignocellulosic bioconversions in solid state fermentation. Brazilian Archives of Biology and Technology 41, 379-390.
Pastore, G.M., Park, Y.K. & Min, D.B. 1994 Production of a fruity aroma by Neurospora from beiju. Mycological Research 98, 25-35.
Saucedo-Castanñda, G., Trejo-Hernández, M.R., Lonsane, B.K., Navarro, J.M., Roussos, S., Dufour, D. & Raimbault, M. 1994 On-line automated monitoring and control systems for CO2 and O2 aerobic and anaerobic solid state fermentations. Process Biochemistry 29, 13-24.
Soares, M., Christen, P., Pandey, A. & Soccol, C.R. 2000 Fruity flavour production by Ceratocystis fimbriata grown on coffee husk in solid state fermentation. Process Biochemistry 35, 857-861.
Sugawara, E., Hashimoto, S., Sakurai, Y. & Kobayashi, A. 1994 Formation by yeast of the HEMF (4-hydroxy-2 (or 5)-ethyl-5 (or 2)-methyl-3 (2H)-furanone) aroma components in Miso with aging. Bioscience Biotechnology and Biochemistry 58, 1134-1135.
Yamauchi, H., Akita, O., Obata, T., Amachi, T., Hara, S. & Yoshizawa, K. 1989 Production and application of a fruity odor in a solid state culture of Neurospora sp. using pre-gelatinized polish rice. Agricultural and Biological Chemistry 53, 2881-2888.
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Medeiros, A.B., Pandey, A., Christen, P. et al. Aroma compounds produced by Kluyveromyces marxianus in solid state fermentation on a packed bed column bioreactor. World Journal of Microbiology and Biotechnology 17, 767–771 (2001). https://doi.org/10.1023/A:1013596330389
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DOI: https://doi.org/10.1023/A:1013596330389