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Phytogard® and DL-β-amino Butyric Acid (BABA) Induce Resistance to Downy Mildew (Bremia Lactucae) in Lettuce (Lactuca sativa L)

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Abstract

Downy mildew of lettuce (Bremia lactucae) is a serious disease. An alternative to chemicals is the application of disease resistance inducers. The aim of this study was to test whether DL-β-amino butyric acid (BABA) and Phytogard® (K2HPO3) could induce resistance in downy mildew susceptible plants. Aqueous solutions of BABA (0, 10, 20, 30, 50, 80, 100 mM) and Phytogard® (0.0, 5.8, 29.0, 40.6, 58.0 and 87.0 ppm) were sprayed on seven-day-old seedlings 0, 3, 7 and 15 days before or 1–3 days after inoculation with B. lactucae. Results obtained showed that Phytogard®- and BABA-induced resistance was dose-dependent. At 40.6 ppm for Phytogard® and 10 mM for BABA, complete protection was obtained. Both compounds had a curative effect and the induced resistance lasted for at least 15 days. It was also shown that both compounds induced systemic resistance in lettuce against downy mildew. Phytogard® at 40.6 ppm completely inhibited spore germination while BABA at 20 mM did not. Pathogenesis related (PR) protein analysis showed that BABA induced weak accumulation of PR-2, but not PR-1, PR-5 and PR-9. Phytogard® induced none of these proteins. The use of these two compounds to protect lettuce from B. lactucae is discussed.

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Authors and Affiliations

  1. Bretagne Biotechnologie Végétale (BBV), Penn-ar-prat, F-29250, Saint Pol-de-Léon, France

    Emmanuel Pajot & Daniel Le Corre

  2. Bretagne Biotechnologie Végétale (BBV), Penn-ar-prat, F-29250, Saint Pol-de-Léon, France

    D. Silué

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  1. Emmanuel Pajot
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  2. Daniel Le Corre
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  3. D. Silué
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Pajot, E., Le Corre, D. & Silué, D. Phytogard® and DL-β-amino Butyric Acid (BABA) Induce Resistance to Downy Mildew (Bremia Lactucae) in Lettuce (Lactuca sativa L). European Journal of Plant Pathology 107, 861–869 (2001). https://doi.org/10.1023/A:1013136608965

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