Skip to main content
Log in

High lipase production by Candida rugosa is associated with G1 cells. A flow cytometry study

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

Growth of C. rugosa on three different culture media was analysed by laser flow cytometry to evaluate physiological growth conditions allowing effective lipase production. The highest productivity was associated with an increased proportion of cells in the G1 phase and was independent of the effect of the medium on lipase formation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Alberghina L, Porro D (1993) Quantitative flow cytometry: analysis of protein distribution in budding yeast. A mini-review. Yeast 9: 815–823.

    PubMed  Google Scholar 

  • Benjamin S, Pandey A (1998) Candida rugosa lipases: molecular biology and versatility in biotechnology. Yeast 14: 1069–1087.

    PubMed  Google Scholar 

  • Chang RC, Chou SJ, Shaw JF (1994) Multiple forms and functions of Candida rugosa lipase. Biotechnol. Appl. Biochem. 19: 93–97.

    Google Scholar 

  • Dalmau E, Montesinos JL, Lotti M, Casas C (2000) Effect of different carbon sources on lipase production by Candida rugosa. Enzyme Microb. Technol. 26: 657–663.

    PubMed  Google Scholar 

  • Del Rio JL, Serra P, Valero F, Poch M, Solà C (1990) Reaction scheme of lipase production by Candida rugosa growing on olive oil. Biotechnol. Lett. 12: 835–838.

    Google Scholar 

  • Eitzman PD, Srienc F (1991) Dynamics of activation of a galactoseinducible promoter in Saccharomyces cerevisiae. J. Biotechnol. 21: 63–81.

    PubMed  Google Scholar 

  • Gordillo MA, Sanz A, Sanchez A, Valero F, Montesinos JL, Lafuente J, Sola C (1998) enhancement of Candida rugosa lipase production by using different control fed-batch operational strategies. Biotechnol. Bioeng. 60: 156–168.

    PubMed  Google Scholar 

  • Lee GC, Tang SJ, Sun KH, Shaw JF (1999) Analysis of the gene family encoding lipases in Candida rugosa by competitive reverse transcription-PCR. Appl. Environ. Microbiol. 65: 3888–3895.

    PubMed  Google Scholar 

  • Lotti M, Monticelli S, Montesinos JL, Brocca S, Valero F, Lafuente J (1998) Physiological control on the expression and secretion of Candida rugosa lipase. Chem. Phys. Lipids 93: 143–148.

    PubMed  Google Scholar 

  • Montesinos JL, Obradors N, Gordillo MA, Valero F, Lafuente J, Solà C (1996) Effect of nitrogen sources in batch and continuous cultures to lipase production by Candida rugosa. Appl. Biochem. Biotechnol. 59: 25–37.

    Google Scholar 

  • Obradors N, Montesinos JL, Valero F, Lafuente J, Solà C (1993) Effect of different fatty acids in lipase production by Candida rugosa. Biotechnol. Lett. 15: 357–360.

    Google Scholar 

  • Porro D, Smeraldi C, Ranzi BM, Martegani E, Alberghina L (1994) Flow cytometric procedures for the analysis of respiratory activity in growing budding yeast. Biotechnol. Prog. 10: 193–197.

    Google Scholar 

  • Porro D, Venturini M, Brambilla L, Alberghina L., Vanoni M (2000) Relating growth dynamics and glucoamylase excretion of individual Saccharomyces cerevisiae cells. J. Microbiol. Meth. 42: 49–55.

    Google Scholar 

  • Ranzi BM, Porro D, Compagno C Martegani E (1987) Protein and cell volume distributions during the production of β-galactodidase in batch cultures of K. lactis. J. Biotechnol. 5: 227–231.

    Google Scholar 

  • Vanoni M, Vai M, Popolo L, Alberghina L (1983) Structural heterogeneity in populations of the budding yeast Saccharomyces cerevisiae. J Bacteriol. 156: 1282–1291.

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Marina Lotti.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lotti, M., Brocca, S. & Porro, D. High lipase production by Candida rugosa is associated with G1 cells. A flow cytometry study. Biotechnology Letters 23, 1803–1808 (2001). https://doi.org/10.1023/A:1012429713782

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1012429713782

Navigation