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Tobacco and Arabidiopsis SLT1 mediate salt tolerance of yeast

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Abstract

A tobacco cDNA (NtSLT1, for Nicotiana tabacum sodium- and lithium-tolerant) was isolated by functional complementation of the salt-sensitive phenotype of a calcineurin (CaN)-deficient yeast mutant (cnbΔ, regulatory subunit null). CaN is a Ca2+/calmodulin-dependent type 2B protein phosphatase that regulates Na+ homeostasis in yeast. This phosphatase modulates plasma membrane K+/Na+ selectivity through the activation of high-affinity K+ transport, and increaseses extracellular Na+ efflux by activation and transcriptional induction of the Na+/Li+translocating P-type ATPase encoded by ENA1. Expression of N-terminally truncated NtSLT1 (Met-304), but not full-length protein, suppressed salt sensitivity of cnb1. Truncated NtSLT1 also increased salt tolerance of wild-type yeast, indicating functional sufficiency. NtSLT1 encodes a protein of yet unknown function but experimentation in yeast confirms it as a salt tolerance determinant. The Arabidopsis thaliana orthologue, AtSLT1, also suppressed salt sensitivity of cnbΔ but only when expressed without the N-terminus (Met-301), suggesting that this region of the proteins from these evolutionarily diverse plant species contains an autoinhibitory domain. NtSLT1 enhanced transcription of the CaN-dependent ENA1 gene promoter and compensated the salt sensitivity of a mutant deficient in TCN1 - a transcription factor that is activated by CaN and then induces ENA1 expression. NtSLT1 partially suppressed the salt sensitivity of ena1-4 indicating that NtSLT1 has both ENA-dependent and independent functions. NtSLT1 suppressed spk1 hal4 (SPK1/HAL4 which encodes a serine-threonine kinase that regulates TRK1-2 transporters to have high K+/Na+ selectivity) but not ena1-4 trk1-2 implicating the ENA-independent function to be through TRK1-2. Together, these results implicate SLT1 as a signal regulatory molecule that mediates salt tolerance by modulating Na+ homeostasis.

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Authors and Affiliations

  1. Center for Plant Environmental Stress Physiology, Department of Horticulture and Landscape Architecture, Purdue University, 1165 Horticulture Building, West Lafayette, IN 47907-1165, USA

    Tracie K. Matsumoto, Ray A. Bressan & Paul M. Hasegawa

  2. Consejo Superior de Investigaciones Cientificas, Instituto de Recursos Naturales y Agrobiologia, Avenida Reina Mercedes, 10, Sevilla , 41012, Spain

    Jose M. Pardo

  3. Department of Environmental Sciences, Faculty of Science, Osaka Women's University, Daisen-cho, Sakai, Osaka, 590-0035, Japan

    Satomi Takeda

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  1. Tracie K. Matsumoto
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  2. Jose M. Pardo
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  3. Satomi Takeda
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  4. Ray A. Bressan
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  5. Paul M. Hasegawa
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Matsumoto, T.K., Pardo, J.M., Takeda, S. et al. Tobacco and Arabidiopsis SLT1 mediate salt tolerance of yeast. Plant Mol Biol 45, 489–500 (2001). https://doi.org/10.1023/A:1010659207604

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