Abstract
Multifluor-fluorescence in-situ hybridization (M-FISH) chromosome paints for all the chromosomes in the human complement labeled with different combinations of fluorochromes is a recent technological development enabling assignment of chromosomal material to rearranged chromosomes. Little data is available on the accuracy and limitations of the approach to the analysis of complex karyotypes, which are characteristic of many malignant diseases. Here we compare M-FISH analysis of the breast-cancer-derived cell line T-47D with a previous analysis by reverse chromosome painting analysis of flow-sorted chromosomes from the same material. This demonstrated a high degree of concordance. It also illustrated the limitations of M-FISH analysis, including difficulties identifying small regions of chromosomal material and intrachromosomal rearrangements. Confirmation of selected aberrations using less-complex mixtures of painting probes and further definition of abnormalities using single copy markers may be required. The detailed karyotype description possible by M-FISH analysis contrasts with the definition in the original G-banding analysis. This and the level of concordance with reverse FISH painting supports the utility of the approach in the definition of complex karyotypes.
Similar content being viewed by others
References
Kallioniemi A, Kallioniemi OP, Sudar D et al. (1992) Comparative genomic hybridization for molecular cytogenetic analysis of solid tumors. Science 258: 818–821.
Keydar I, Chen L, Karby S et al. (1979) Establishment and characterization of a cell line of human breast carcinoma origin. Eur J Cancer 15: 659–670.
Macville M, Schrock E, Padilla-Nash H et al. (1999) Comprehensive and definitive molecular cytogenetic characterization of Hela cells by spectral karyotyping. Cancer Res 59: 141–150.
Morris JS, Carter NP, Ferguson-Smith MA, Edwards PAW (1997) Cytogenetic analysis of three breast carcinoma cell lines using reverse chromosome painting. Genes Chromosomes Cancer 20: 120–139.
Schrock E, du Manoir S, Veldman T et al. (1996) Multicolor spectral karotyping of human chromosomes. Science 273: 494–497.
Speicher MR, Ballard SG, Ward DC (1996) Karyotyping human chromosomes by combinatorial multi-fluor FISH. Nat Genet 12: 368–375.
Tosi S, Giudici G, Rambaldi A et al. (1999) Characterization of the human myeloid leukemia-derived cell line GF-D8 by multiplex fluorescence in situ hybridization, subtelomeric probes, and comparative genomic hybridization. Genes Chromosomes Cancer 24: 213–221.
Uhrig S, Schuffenhauer S, Fauth C et al. (1999) Multiplex-FISH for pre-and postnatal diagnositc applications. Am J Hum Genet 65: 448–462.
Veldman T, Vignon C, Schrock E, Rowley JD, Ried T (1997) Hidden chromosome abnormalities in haematological malignancies detected by multicolour spectral karyotyping. Nat Genet 15: 406–410.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Lu, YJ., Morris, J.S., Edwards, P.A.W. et al. Evaluation of 24-color Multifluor-Fluorescence In-situ Hybridization (M-FISH) Karyotyping by Comparison with Reverse Chromosome Painting of the Human Breast Cancer Cell Line T-47D. Chromosome Res 8, 127–132 (2000). https://doi.org/10.1023/A:1009242502960
Issue Date:
DOI: https://doi.org/10.1023/A:1009242502960