Abstract
Broiler chicks of both sexes were fed formulated rations containing different fibre types with and without supplementation with crude and pure microbial enzymes in order to determine the effect on the lipids of the blood, liver and kidney. Results shown enzyme supplementation to greatly and significantly affect total lipid, free fatty acids, triglyceride and cholesterol levels of the blood, liver and kidney relative to the age of the birds and the type of fibres. By day 42, the total blood lipid of the unsupplemented diet rose to the highest level (22.4 mg/l), that of the kidney (3.62 mg/g) and the liver (185.7 mg/g). Enzyme supplementation, however, brought about reduction of these values with the diet containing all the fibres – wheat bran, corn bran, palm kernel meal and coconut meal giving the lowest values of the total lipids, free fatty acids, triglycerides and cholesterol on day 42 for the blood, liver and kidney, respectively. The pure forms of the enzymes applied directly to the feed gave more highly significant effects than the crude forms fed through water. Interaction effects between the various forms of the enzyme and the mode of application were also statistically significant.
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References
Cheetham, P.S.J. 1984 Development in the immobilization of microbial cells and their applications. In Topics in Enzyme and Fermentation Biotechnology. Vol. 4, ed. Wiseman, A. pp. 189–238. Chichester: Ellis Horwood Ltd.
Chen, W.J.L. & Anderson, J.W. 1986 Hypocholesterolaemic effects of soluble fibre. In Dietary Fiber: Basic and Clinical Aspects, eds Vahouny, G.V. & Kritchevy, D. pp. 275–286. New York: Plenum. ISBN 0–30642028–7.
Chesson, A. 1987 Supplementary enzymes to improve the utilization of pig and poultry diets. In Recent Advances in Animal Nutrition, eds Haresign, W. & Cole, D.J.A. pp. 71–89. London: Butterworths. ISBN 0–40701163–3.
Corredor, C., Mansbach, C. & Bressler, R. 1967 Carnitine depletion in the choline-deficient state. Biochimica et Biophysica Acta 144, 366–374.
Duncan, D.B. 1955 Multiple range and multiple F-test. Biometrics 11, 1–42.
Fletcher, M.J. 1968 A colorimetric method for estimating serum triglyceride. Clinica Chimica Acta 22, 393–397.
Folch, J., Lees, M. & Sloane-Stanley, E.H. 1957 A simple method for the isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry 226, 497–509.
Hammer, R.J. 1989 Aspects of improving the nutritional values of cereal grains for monogastrics. In Forum Feed Symposium on Feed Enzymes in Animal Production Proceedings. Solihull, England.
Hansen, I., Bach Knudsen, K.E. & Eggum, B.O. 1992 Gastrointestinal implications in the rat of wheat bran, oat bran and pea fibre. British Journal of Nutrition 68, 451–462.
Havel, R.J., Felts, J.M. & Van Duyne, C.M. 1962 Formation and fate of endogenous triglycerides in blood plasma of rabbits. Journal of Lipid Research 3, 297–308.
Hesselman, K. 1983 Effects of β-glucanase supplementation to barley-based diets for broiler chicks. Report 112 Sveriges Lantbruksununiversites, Uppsala.
Hesselman, K. & Aman, P. 1986 The effect of β-glucanase on the utilisation of starch and nitrogen by broiler chickens fed on barley of low-or high viscosity. Animal Feed Science and Technology 15, 83–93.
Kay, R.M. & Truswell, A.S. 1977 Effect of citrus pectin on blood lipids and faecal steroid excretion in man. American Journal of Clinical Nutrition 30, 171–175.
Kim, E. & Goldberg, M. 1969 Serum Cholesterol assay using a stable Liebermann-Burchard reagent. Clinical Chemistry 15, 1171–1179.
Lombardi, B. 1971 Effect of choline deficiency on rat hepatocytes. Federation Proceedings 30, 139–142.
Maner, J.H., Pond, W.G., Loosli, J.K. & Lowery, R.S. 1962 Effects of isolated soybean protein and casein on the gastric pH and rate of passage of food residues in baby pigs. Journal of Animal Science 21, 49–52.
Mateos, G.G., Jerry, I.S. & Jennifer, A.E. 1982 Rate of food passage (transit time) as influenced by level of supplemental fat. Poultry Science 61, 94–100.
MacDonald, P., Edwards, R.A. & Greenhalgh, J.F.D. 1981 Animal Nutrition, 3rd edn. New York: Longman.
Mookerjea, S. 1971 Action of choline in lipoprotein metabolism. Federation Proceedings 30, 143–150.
Ogunlayi, B.A. 1994 Personal communication. Department of Animal Science, University of Ibadan, Ibadan, Nigeria.
Onilude, A.A. & Oso, B.A. 1999 Effect of fungal enzyme mixture supplementation of various dietary fibre-containing diets fed broiler chicks. 1: Performance and carcass characteristics. World Journal of Microbiology & Biotechnology 15, 353–360.
Ontko, J.A. 1972 Metabolism of free fatty acids in isolated liver cells. Journal of Biological Chemistry 247, 1788–1800.
Pearson, D. 1976 The Chemical Analysis of Foods, 7th edn. London: Churchill Livingstone Publishers. ISBN 0–44301411–6.
Pettersson, D. & Aman, P. 1991 Production results, serum cholesterol concentration and carcass composition of broiler chickens fed diets based on bran or inner endosperm from oats with or without enzyme supplementation. Journal of the Science of Food & Agriculture 57, 273–286.
Sen, K.C., Ray, S.N. & Ranjhan, S.K. 1978 The nutritive value of cattle feeds and nutrition of farm animals. Delhi: ICAR.
Tietz, N.W. 1976 Fundamentals of Clinical Chemistry. 2nd edn. Philadelphia: W.B. Saunders Company. ISBN 0–72168866–7.
Topping, D.L. & Mayes, P.A. 1972 The immediate effects of insulin and fructose on the metabolism of the perfused liver: changes in lipoproteins secretion, fatty acid oxidation and esterification, lipogenesis and carbohydrate metabolism. Biochemical Journal 126, 295–311.
White, A., Handler, P. & Smith, E.L. 1973 Principles of Biochemistry, 7th edn. Tokyo: McGrawHill Kogakusha Ltd. ISBN 0–07–069758–2.
Whitehead, C.C. 1975 Tissue lipid composition in fatty liver and kidney syndrome in chicks. Researches in Veterinary Science 18, 32–35.
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Onilude, A., Oso, B. Effect of fungal enzyme mixture supplementation of various fibre-containing diets fed to broiler chicks 2: On blood, liver and kidney total lipids, triacylglycerols and cholesterol. World Journal of Microbiology and Biotechnology 15, 315–320 (1999). https://doi.org/10.1023/A:1008987919600
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DOI: https://doi.org/10.1023/A:1008987919600