Abstract
The effect of various carbon and nitrogen sources on cellulose membrane production by Acetobacter xylinum was evaluated. Among the carbon sources, sucrose, glucose and mannitol were found to be suitable for optimum levels of cellulose production. The strain was able to utilize a wide range of protein and nitrogen sources such as peptone, soybean meal, glycine, casein hydrolysate, and glutamic acid for cellulose synthesis. Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) analysis of pellicle proteins (PP) revealed electrophoretic bands of molecular masses in the range of 116–20 kDa. Furthermore, the strain can be useful for the removal of various nitrogenous and carbon substrates present in waste waters.
Article PDF
Similar content being viewed by others
References
Masaoka, S., Ohe, T. & Sakota, N. 1993 Production of cellulose from glucose by Acetobacter xylinum. Journal of Fermentation and Bioengineering 75, 18-22.
Matsuoka, M., Tsuchida, T., Matsushita, K., Adachi, O. & Yoshinaga, F. 1996 A synthetic medium for bacterial cellulose production by Acetobacter xylinum sub sp sucrofermentans. Bioscience Biotechnology and Biochemistry 60, 575-579.
Singh, L., Ramana, K.V., Banerjee, S., Dube, V. & Chauhan, R.S. 1996 Studies on bacterial cellulose membrane production and its structural properties. Proceedings of the XIV National Symposium on Membranes in Chemical and Biochemical Industries, Indian Institute of Technology, Delhi, India, pp. 31-34.
Watanabe, K. & Yamanaka, S. 1995 Effect of oxygen tension in the gaseous phase on production and physiological properties of bacterial cellulose formed under static culture conditions. Bioscience Biotechnology and Biochemistry 59, 65-68.
White, D.G. & Brown, R.M. 1989 Prospects for the commercialization of the biosynthesis of microbial cellulose. In Cellulose and Wood-Chemistry and Technology, ed Schuerch, C. pp. 573-590. John Wiley & Sons Inc., New York. ISBN0-471-51256-7.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ramana, K., Tomar, A. & Singh, L. Effect of various carbon and nitrogen sources on cellulose synthesis by Acetobacter xylinum. World Journal of Microbiology and Biotechnology 16, 245–248 (2000). https://doi.org/10.1023/A:1008958014270
Issue Date:
DOI: https://doi.org/10.1023/A:1008958014270