Abstract
The objective was to obtain the polysaccharide fraction present in the extracted polymeric matrix of a biofilm by differential precipitation of the protein content. This was tried by pH adjustment and by addition of trichloroacetic acid (TCA). The best yield of protein precipitation was with 20% (w/v) TCA, with more than 97% of the protein removed and without affecting the amount of polysaccharides in solution.
Similar content being viewed by others
References
Beri RG, Rollings JE (1995) A technique for the rapid removal of proteins from cell extracts containing polysaccharides. Biotechnol. Tech. 9: 783-786.
Doco T, Carcano D, Ramos P, Loones A, Fournet B (1991) Rapid isolation and estimation of polysaccharide from fermented skim milk with Streptococcus salivarius subsp. thermophilus by coupled anion exchange and gel-permeation high-performance liquid chromatography. J. Dairy Res. 5: 147-150.
Dubois, M, Gilles, KA, Hamilton, JK, Rebers PA, Smith F (1956). Colorimetric method for determination of sugars and related substances. Anal. Chem. 28: 350-356.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Oliveira, R., Marques, F. & Azeredo, J. Purification of polysaccharides from a biofilm matrix by selective precipitation of proteins. Biotechnology Techniques 13, 391–393 (1999). https://doi.org/10.1023/A:1008954301470
Issue Date:
DOI: https://doi.org/10.1023/A:1008954301470