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Purification of polysaccharides from a biofilm matrix by selective precipitation of proteins

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Biotechnology Techniques

Abstract

The objective was to obtain the polysaccharide fraction present in the extracted polymeric matrix of a biofilm by differential precipitation of the protein content. This was tried by pH adjustment and by addition of trichloroacetic acid (TCA). The best yield of protein precipitation was with 20% (w/v) TCA, with more than 97% of the protein removed and without affecting the amount of polysaccharides in solution.

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Oliveira, R., Marques, F. & Azeredo, J. Purification of polysaccharides from a biofilm matrix by selective precipitation of proteins. Biotechnology Techniques 13, 391–393 (1999). https://doi.org/10.1023/A:1008954301470

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  • DOI: https://doi.org/10.1023/A:1008954301470

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