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Non-structural plum pox potyvirus proteins detected by immunogold labelling

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Abstract

Plum pox potyvirus (PPV) induces in infected Nicotiana clevelandii cells characteristic crystalline inclusions known as nuclear inclusions (NI) when located in the nucleus and as dense material (Dm) when located in the cytoplasm. Crystalline inclusions contain protease (NIa) and RNA-dependent RNA polymerase (NIb) proteins. It is now well established for all potyviruses that cylindrical inclusions contain CI helicase ATPase protein (Martin et al., 1992). The intracellular location of other non-structural PPV proteins remains unknown. Using Escherichia coli expression vectors, specific antibodies were obtained against P1, P3, 6K2 and NIb PPV proteins for which antibodies were not yet available. As expected, NIb antiserum labelled crystalline inclusions. P1, P3 and 6K2 proteins were present in both types of crystalline inclusions found in the nucleus and in the cytoplasm of PPV-infected leaves of N. clevelandii, suggesting that nuclear inclusions and dense material were composed of the same proteins. This composition is discussed.

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Authors and Affiliations

  1. Laboratoire de biologie cellulaire, route de Saint Cyr, 78026, Versailles cedex, France

    M.T. Martin

  2. Laboratoire de pathologie végétale I.N.R.A., route de Saint Cyr, 78026, Versailles cedex, France

    B. Gélie

Authors
  1. M.T. Martin
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  2. B. Gélie
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Martin, M., Gélie, B. Non-structural plum pox potyvirus proteins detected by immunogold labelling. European Journal of Plant Pathology 103, 427–431 (1997). https://doi.org/10.1023/A:1008653000005

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  • DOI: https://doi.org/10.1023/A:1008653000005

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