Abstract
A collection of 127 strains of Erwinia amylovora, the causative agent of fire blight, was tested by PCR amplification of a fragment of the plasmid pEA29. A variability in the length of the DNA fragment obtained was observed after digestion by MspI and Sau3A restriction enzymes. Strains were distributed into three groups according to the length of the DNA product. Most of the strains analysed were placed into two groups. Thirteen strains were clustered into a third group which was linked with the geographical origin of strains: they were all isolates from recently reported outbreaks of fire blight in Austria and in southern Bavaria in Germany. The variation in the length of the amplified fragment is probably due to an insertion into this fragment.
References
Anonymous (1996) Premier signalement d'Erwinia amylovora en Hongrie. OEPP/EPPO Reporting Service 96/106
Barny MA, Guinebretière MH, Marçais B, Coissac E, Paulin JP and Laurent J (1990) Cloning a large gene cluster involved in Erwinia amylovora CFBP 1430 virulence. Molecular Microbiology 4: 777–786
Bereswill S, Pahl A, Bellemann P, Zeller W and Geider K (1992) Sensitive and species-specific detection of Erwinia amylovora by polymerase chain reaction analysis. Applied and Environmental Microbiology 58 (11): 3522–3526
Bereswill S, Pahl A, Bellemann P, Berger F, Zeller W and Geider K (1993) Efficient detection of Erwinia amylovora by PCR-analysis. Acta Horticulturae 338: 51–58
Falkenstein H, Bellemann P, Walter S, ZellerWand Geider K (1988) Identification of Erwinia amylovora, the fireblight pathogen, by colony hybridization with DNA from plasmid pEA29. Applied and Environmental Microbiology 54: 2798–2802
Falkenstein H, Zeller W and Geider K (1989) The 29 kb plasmid, common in strains of Erwinia amylovora, modulates development of fireblight symptoms. Journal of General Microbiology 135: 2643–2650
Hale CN and Clark RC (1990) Detection of Erwinia amylovora from apple tissue by DNAhybridization. Acta Horticulturae 273: 51–55
Keck M(1994) Erstes Feuerbrand-Auftreten in Ôsterreich-Vorsicht im Kernobstbau, Bessres Obst 1: 25–26
Keck M, Chartier R, Reich H and Paulin JP (1996) First record of fire blight (Erwinia amylovora) in Austria-Preliminary experiments on the survival on fruit boxes. Acta Horticulturae 411: 9–12
King EO, Ward MK and Raney DE (1954) Two simple media for the demonstration of pyocyanin and fluorescein. Journal of Laboratory Clinical Medicine 44: 301–397
Klement Z (1990) Inoculation of plant tissues: introduction and general instructions. Chapter 1.5. In: Klement Z, Rudolph K and Sands DC (eds)Methods in Phytobacteriology (pp. 95–124) Akademiai Kiado, Budapest
Laurent J, Barny MA, Kotoujansky A, Dufriche P and Vanneste J (1989) Characterization of a ubiquitous plasmid in Erwinia amylovora. Molecular Plant-Microbe Interactions 2(4): 160–164
Maniatis T, Fritsch EF and Sambrook J (1982) Molecular cloning, a laboratory manual. Cold Spring Harbor Laboratory, Box 100, Cold Spring Harbour, New York.
McManus PS and Jones AL (1995) Detection of Erwinia amylovora by nested PCR and PCR-dot-blot and reverse-blot hybridizations. Phytopathology 85: 618–623
Tharaud M, Menggad M, Paulin JP and Laurent J (1994) Virulence, growth, and surface characteristics of Erwinia amylovora mutants with altered pathogenicity. Microbiology 140: 659–669
Vanneste JL (1995) Erwinia amylovora. In: Singh US, Singh RP and Kohmoto K (eds) Pathogenesis and Host Specificity in Plant Diseases: Histopathological, Biochemical, Genetic and Molecular Bases. Vol. 1 Prokaryotes (pp. 21–41) Pergamon Press, Oxford, London
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Lecomte, P., Manceau, C., Paulin, JP. et al. Identification by PCR analysis on plasmid pEA29 of isolates of Erwinia amylovora responsible of an outbreak in Central Europe. European Journal of Plant Pathology 103, 91–98 (1997). https://doi.org/10.1023/A:1008607226805
Issue Date:
DOI: https://doi.org/10.1023/A:1008607226805