Abstract
Media employed for imparting partial alignment onto solute molecules have recently attracted considerable attention, since they permit the measurement of NMR parameters for solute biomolecules commonly associated with solid state NMR. Here we characterize a medium which is based on a quasi-ternary surfactant system comprising cetylpyridinium bromide/hexanol/sodium bromide. We demonstrate that dilute solutions of this system can exist in liquid crystalline phases which orient in the magnetic field and allow the measurement of residual dipolar couplings under a variety of conditions. The present system is extremely versatile and robust, tolerating different buffer conditions, temperature ranges and concentrations.
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Bax, A. and Tjandra, N. (1997) J. Biomol. NMR, 10, 289–292.
Ben-Shaul, A., Rorman, D.H., Hartland, G.V. and Gelbart, W.M. (1986) J. Phys. Chem., 90, 5277–5286.
Benton, W.J. and Miller, C.A. (1983) J. Phys. Chem., 87, 4981–4991.
Cavagnero, S., Dyson, J.H. and Wright, P.E. (1999) J. Biomol. NMR, 13, 387–391.
Clore, G.M. and Gronenborn, A.M. (1989) CRC Crit. Rev. Biochem. Mol. Biol., 24, 479–564.
Clore, G.M., Starich, M.R. and Gronenborn, A.M. (1998) J. Am. Chem. Soc., 120, 10571–10572.
Derick, J.P. and Wigley, D.B. (1994) J. Mol. Biol., 243, 906–918.
Gomati, R., Appell, J., Bassereau, P., Marignan, J. and Porte, G. (1987) J. Phys. Chem., 91, 6203–6210.
Gronenborn, A.M., Filpula, D.R., Essig, N.Z., Achari, A., Whitlow, M., Wingfield, P.T. and Clore, G.M. (1991) Science, 253, 657–661.
Hansen, M.R., Rance, M. and Pardi, A. (1998a) J. Am. Chem. Soc., 120, 11210–11211.
Hansen, M.R., Mueller, L. and Pardi, A. (1998b) Nat. Struct. Biol., 5, 1065–1074.
Helfrich, W. (1978) Z. Naturforsch., C33, 305–315.
Hoffmann, H. (1994) Ber. Bunsenges. Phys. Chem., 98, 1433–1455.
Koenig, B.W., Hu, J.-S., Ottiger, M., Bose, S., Hendler, R.W. and Bax, A. (1999) J. Am. Chem. Soc., 121, 1385–1386.
Kung, H.C., Wang, K.Y., Golier, I. and Bolton, P.H. (1995) J. Magn. Reson., B109, 323–325.
Losonczi, J.A. and Prestegard, J.H. (1998) J. Biomol. NMR, 12, 447–451.
McGrath, K.M. (1997) Langmuir, 13, 1987–1995.
Nakamura, H. and Okano, K. (1983) Phys. Rev. Lett., 50, 186–189.
Ottiger, M. and Bax, A. (1998) J. Biomol. NMR, 12, 361–372.
Ottiger, M. and Bax, A. (1999) J. Biomol. NMR, 13, 187–191.
Porte, G., Appell, J. and Poggi, Y. (1980) J. Phys. Chem., 84, 3105–3110.
Porte, G. and Appell, J. (1981) J. Phys. Chem., 85, 2511–2519.
Porte, G., Gomati, R., El Haitamy, O., Appell, J. and Marignan, J. (1986) J. Phys. Chem., 90, 5746–5751.
Prestegard, J.H. (1998) Nat. Struct. Biol., 5, Suppl, 517–522.
Prosser, R.S., Losonczi, J.A. and Shiyanovskaya, I.V. (1998) J. Am. Chem. Soc., 120, 11010–11011.
Sanders, C.R. and Prosser, R.S. (1998) Structure, 6, 1227–1234.
Sass, J., Cordier, F., Hoffmann, A., Rogowski, M., Cousin, A., Omichinski, J.G., Lowen, H. and Grzesiek, S. (1999) J. Am. Chem. Soc., 121, 2047–2055.
Tjandra, N. (1999) Structure, 7, R 205–211.
Tjandra, N. and Bax, A. (1997a) Science, 278, 1111–1114.
Tjandra, N. and Bax, A. (1997b) J. Magn. Reson., 124, 512–515.
Tjandra, N., Grzesiek, S. and Bax, A. (1996) J. Am. Chem. Soc., 118, 6264–6272.
Tjandra, N., Omichinski, J.G., Gronenborn, A.M., Clore, G.M. and Bax, A. (1997) Nat. Struct. Biol., 4, 732–738.
Tolman, J.R., Flanagan, J.M., Kennedy, M.A. and Prestegard, J.H. (1995) Proc. Natl. Acad. Sci. USA, 92, 9279–9283.
Tolman, J.R. and Prestegard, J.H. (1996a) J. Magn. Reson., B112, 245–252.
Tolman, J.R. and Prestegard, J.H. (1996b) J. Magn. Reson., B112, 269–274.
Wang, H., Eberstadt, M., Olejniczak, E.T., Meadows, R.P. and Fesik, S.W. (1998a) J. Biomol. NMR, 12, 443–446.
Wang, Y.-X., Marquardt, J.L., Wingfield, P., Stahl, S.J., Lee-Huang, S., Torchia, D. and Bax, A. (1998b) J. Am. Chem. Soc., 120, 7385–7386.
Wüthrich, K. (1986) NMR of Proteins and Nucleic Acids, Wiley, New York, NY.
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Barrientos, L.G., Dolan, C. & Gronenborn, A.M. Characterization of surfactant liquid crystal phases suitable for molecular alignment and measurement of dipolar couplings. J Biomol NMR 16, 329–337 (2000). https://doi.org/10.1023/A:1008356618658
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DOI: https://doi.org/10.1023/A:1008356618658