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An anticoagulant proteoglycan from the marine green alga, Codium pugniformis

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Abstract

An anticoagulant isolated from the marine green alga Codium pugniformis was composed mainly of glucose with minor amounts of arabinose and galactose. It was highly sulfated (326 μg mg-1 polysaccharide) and contained protein(52 μg mg-1 polysaccharide) and was thus a proteoglycan. The anticoagulant properties of the purified proteoglycan were compared with those of heparin by studying the activated partial thromboplastin time (APTT), prothrombin time (PT) and thrombin time(TT) using normal human plasma. The proteoglycan showed similar activities to heparin, but was weaker than heparin. On the other hand, the proteoglycan did not affect PT even at the concentration at which APTT and TT were prolonged. The anticoagulation mechanism of this proteoglycan was due to the direct inhibition of thrombin and the potentiation of antithrombin III.

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References

  • Church FC, Maeda JB, Treanor RE, Whinna HC (1989) Antithrombin activity of fucoidan. J. biol. Chem. 264: 3618-3623.

    PubMed  CAS  Google Scholar 

  • Colliec S, Fischer AM, Tapon-Bretaudiere J, Boisson C, Durand P, Jozefonvicz J (1991) Anticoagulant properties of a fucoidan fraction. Thromb. Res. 64: 143-154.

    Article  PubMed  CAS  Google Scholar 

  • Deacon-Smith RA, Lee-Potter JP, Rogers DJ (1985) Anticoagulant activity in extracts of British marine algae. Bot. mar. 28: 333-338.

    Article  Google Scholar 

  • Doctor VM, Hill C, Jackson GJ (1995) Effect of fucoidan during activation of human plasminogen. Thromb. Res. 79: 237-247.

    Article  PubMed  CAS  Google Scholar 

  • Dubois M, Gilles, KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Analyt. Chem. 28: 350-356.

    Article  CAS  Google Scholar 

  • Jurd KM, Mclellan DS, Rogers DJ, Blunden G (1990) The anticoagulantactivities of aqueous extracts from Codium fragile sub sp tomentosoides. Br. J. Haematol. 76: 25.

    Google Scholar 

  • Jurd KM, Rogers DJ, Blunden G, McLellan DS (1995) Anticoagulant properties of sulfated polysaccharides and a proteoglycan from Codium fragile ssp. atlanticum. J. appl. Phycol. 7: 339-345.

    Article  CAS  Google Scholar 

  • Maeda M, Uehara T, Harada N, Sekiguchi M, Hiraoka A (1991) Heparinoid-active sulfated polysaccharides from Monostroma nitidum and their distribution in the Chlorophyta. Phytochemistry 30: 3611-3614.

    Article  CAS  Google Scholar 

  • Maraganore JM, Chao B, Joseph ML, Jablonski J, Ramachandran KL (1989) Anticoagulant activity of synthetic hirudin peptides J. biol. Chem. 264: 8692-8698.

    PubMed  CAS  Google Scholar 

  • McLellan DS, Jurd KM (1992) Anticoagulants from marine algae. Blood Coagulation and Fibrinolysis 3: 69-77.

    PubMed  CAS  Google Scholar 

  • Mourâno PAS, Pereira MS, Pavao MSG, Mulloy B, Tollefsen DM, Mowinckel MC, Abildgaard U (1996) Structure and anticoagulant activity of a fucosylated chondroitin sulfate from echinoderm. J. biol. Chem. 271: 23973-23984.

    Article  Google Scholar 

  • Nishino T, Yokoyama G, Dobashi K, Fujihara M, Nagumo T (1989) Isolation, purification, and characterization of fucose-containing sulfated polysaccharides from the brown seaweed Ecklonia kurome and their blood-anticoagulant activities. Carbohydr. Res. 186: 119-129.

    Article  PubMed  CAS  Google Scholar 

  • Pereira MS, Mulloy B, Mourâno PAS (1999) Structure and anticoagulant activity of sulfated fucans. J. biol. Chem. 274: 7656-7667.

    Article  PubMed  CAS  Google Scholar 

  • Rogers DJ, Jurd KM, Blunden G, Paoletti S, Zanetti F (1990) Anticoagulant activity of a proteoglycan in extracts of Codium fragile ssp. atlanticum. J. appl. Phycol. 2: 357-361.

    Article  CAS  Google Scholar 

  • Silvestri LJ, Hurst, RE, Simpson L, Settine JM (1982) Analysis of sulfate in complex carbohydrates. Analyt. Biochem. 123: 303-309.

    Article  PubMed  CAS  Google Scholar 

  • Soeda S, Sakaguchi S, Shimeno H, Nagamatsu A (1992) Fibrinolytic and anticoagulant activities of highly sulfated fucoidan. Biochem. Pharmacol. 43: 1853-1858.

    Article  PubMed  CAS  Google Scholar 

  • Uehara T, Takeshita M, Maeda M (1992) Studies on anticoagulantactive arabinan sulfates from the green alga, Codium latum. Carbohydr. Res. 235: 309-311.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Nutritional Science, Faculty of Health and Welfare Science, Okayama Prefectural University, 111 Kuboki, Soja, Okayama, 719-1197, Japan

    Kiminori Matsubara & Yasushi Matsuura

  2. Faculty of Applied Biological Science, Hiroshima University, 1-4-4 Kagamiyama, Higashi-Hiroshima, Hiroshima, 739-8528, Japan

    Kanji Hori & Keisuke Miyazawa

Authors
  1. Kiminori Matsubara
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  2. Yasushi Matsuura
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  3. Kanji Hori
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  4. Keisuke Miyazawa
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Matsubara, K., Matsuura, Y., Hori, K. et al. An anticoagulant proteoglycan from the marine green alga, Codium pugniformis . Journal of Applied Phycology 12, 9–14 (2000). https://doi.org/10.1023/A:1008174115350

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  • DOI: https://doi.org/10.1023/A:1008174115350

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