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A comparison of screening methods for antioxidant activity in seaweeds

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Abstract

The inhibition of lipid peroxidation and radical scavenging effects were studied to evaluate the antioxidant activity for extracts of 17 species of seaweed. The antioxidant effect was evaluated by determination of lipoxygenase activity and by α, α-diphenyl-β-picrylhydrazyl (DPPH) decolorization. Lipoxygenase activity was depressed in the presence of aqueous and ethanol extracts of 4 algal species; Sargassum species had the highest antioxidant activity of all the species examined. The ethanol extracts of one Sargassum species showed competitive inhibition with the substrate. The same species also showed radical scavenging activity in the DPPH decolorization test. Comparison of these results shows no relationship between enzyme inhibition and radical scavenging activity.

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Authors and Affiliations

  1. Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153, Japan

    R. Matsukawa, M. Chihara, Y. Yamamoto, E. Niki & I. Karube

  2. Department of Life Science, Bar-Ilan University, Ramat-Gan, 52900, Israel

    Z. Dubinsky

  3. Faculty of Engineering, The Science University of Tokyo, 1-3, Kagurazaka, Shinjuku-ku, Tokyo, 162, Japan

    E. Kishimoto & Y. Masuda

  4. Toyo Susan Kaisha Ltd, 2-13-40 Kohnan, Minato-ku, 108, Tokyo, Japan

    K. Masaki

  5. Faculty of Information Science, Hiroshima City University, 151-5, Ozuka, Numata-cho, Asaminami-ku, Hiroshima, 731-31, Japan

    T. Takeuchi

  6. Japanese Red Cross College of Nursing, 4-1-3, Hiro-o, Shibuya-ku, Tokyo, 150, Japan

    M. Chihara

Authors
  1. R. Matsukawa
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  2. Z. Dubinsky
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  3. E. Kishimoto
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  4. K. Masaki
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  5. Y. Masuda
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  6. T. Takeuchi
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  7. M. Chihara
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  8. Y. Yamamoto
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  9. E. Niki
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  10. I. Karube
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Matsukawa, R., Dubinsky, Z., Kishimoto, E. et al. A comparison of screening methods for antioxidant activity in seaweeds. Journal of Applied Phycology 9, 29–35 (1997). https://doi.org/10.1023/A:1007935218120

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  • DOI: https://doi.org/10.1023/A:1007935218120

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