Skip to main content
SpringerLink
Log in

Influence of n-3 highly unsaturated fatty acid deficiency on the lipid composition of broodstock gilthead seabream (Sparus aurata L.) and on egg quality

  • Published:
Fish Physiology and Biochemistry Aims and scope Submit manuscript

Abstract

A feeding experiment was conducted on gilthead seabream (Sparus aurata) broodstock to investigate the incidence of n-3 highly unsaturated fatty acids (n-3 HUFA) dietary deficiencies on the lipid composition of female liver, gonads and eggs, in relation to spawning quality. Broodstock were fed a control (C) diet or a n-3 HUFA deficient (D) but linolenic acid rich diet. After 20 weeks of feeding, the results showed that levels of total neutral (TNL) and total polar (TPL) lipids of female gonads and eggs were independent of diet. However the fatty acid composition of phosphatidylcholine (PC), phosphatidylethanolamine (PE) and phosphatidylinositol (PI) of female liver, gonads and eggs in the two groups of fish showed marked differences, reflecting the influence of fatty acid levels in the broodstock diets. This influence was even higher in TNL than in the phospholipid classes examined. In fish fed n-3 HUFA deficient diet, fatty acid composition of TNL of female gonads and eggs reflected the diet more than liver. A higher egg production in broodstock fed C diet (1.8% n-3 HUFA in diet) was extended to spawning quality such as percentages of fertilised and hatched eggs.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References cited

  • Bell, M.V., Henderson, R.J. and Sargent, J.R. 1986. The role of polyunsaturated fatty acids in fish. Comp. Biochem. Physiol. 83B: 711–719.

    Google Scholar 

  • Bell, M.V. 1989. Molecular species analysis of phosphoglycerides from the ripe roes of cod (Gadus morhua). Lipids 24: 585–588.

    Google Scholar 

  • Cejas, J., Villamandos, J. and Samper, M. 1992. Estudio sobre la reproducción de la dorada, Sparus aurata. Influencia del peso/edad de las hembras sobre la calidad de puesta. Instituto Español de Oceanografía. Informes técnicos No 126.

  • Christie, W.W. (Ed.) 1982. Lipid Analysis. Pergamon Press, Oxford.

    Google Scholar 

  • Craik, J.A.C. and Harvey, S.H. 1984. Egg quality in rainbow trout: The relation between eggs viability, selected aspects of egg composition and time of stripping. Aquaculture 40: 115–130.

    Google Scholar 

  • Devauchelle, N., Alexandre, J.C., Le Carre, N. and Letty, Y. 1988. Spawning of turbot (Scophthalmus maximus) in captivity. Aquaculture 69: 159–184.

    Google Scholar 

  • Fernández-Palacios, H., Izquierdo, M., Robaina, L., Valencia, A., Salhi, M. and Vergara, J.M. 1995. Effect of n-3 HUFA level in broodstock diets on egg quality of gilthead seabream (Sparus aurata L.). Aquaculture 132: 325–337.

    Google Scholar 

  • Greene, D.H.S. and Selivonchick, D.P. 1987. Lipid metabolism in fish. Prog. Lipid Res. 26: 53–85.

    Google Scholar 

  • Henderson, R.J. and Tocher, D.R. 1987. The lipid composition and biochemistry of freshwater fish. Prog. Lipid Res. 26: 281–347.

    Google Scholar 

  • Horwitz, W. (Ed.) 1980. Methods of Analysis, 13th edition. Association of Official Analytical Chemists, Washington, DC.

    Google Scholar 

  • Ibeas, C., Izquierdo, M.S. and Lorenzo, A. 1994. Effect of different levels of n-3 highly unsaturated fatty acids on growth and fatty acid composition of juvenile gilthead seabream (Sparus aurata). Aquaculture 127: 177–188.

    Google Scholar 

  • Ibeas, C., Cejas, J.R., Gómez, T., Jerez, S. and Lorenzo, A. 1996. Influence of dietary n-3 highly unsaturated fatty acids levels on juvenile gilthead seabream (Sparus aurata) growth and tissue fatty acid composition. Aquaculture 142: 221–235.

    Google Scholar 

  • Kalogeropoulos, N., Alexis, M.N. and Henderson, R.J. 1992. Effects of dietary soybean and cod-liver oil levels on growth and body composition of gilthead seabream (Sparus aurata). Aquaculture 104: 293–308.

    Google Scholar 

  • Kimata, M. 1983. Changes of fatty acid composition during early development in the red seabream, Chrysophrys major (T.et S.). J. Fac. Har. Sci. Technol. Tokai. Univ. 16: 225–233.

    Google Scholar 

  • Koven, W.M., Kissil, G.W. and Tandler, A. 1989. Lipid and n-3 HUFA requirement of Sparus aurata larvae during starvation and feeding. Aquaculture 79: 185–191.

    Google Scholar 

  • Koven, W.M., Tandler, A., Kissil, G.W., Sklan, D., Friezlander, O. and Harel, M. 1990. The effect of dietary (n-3) polyunsaturated fatty acids on growth, survival and swin bladder development in Sparus aurata larvae. Aquaculture 91: 131–141.

    Google Scholar 

  • Lasker, R. and Theilaker, G.H. 1962. The fatty acid composition of lipids of some Pacific sardine tissues in relation to ovarian maduration and diet. J. Lipid Res. 3: 60–64.

    Google Scholar 

  • Lie, Ø. and Lambertsen, G. 1991. Fatty acid composition of glycerophospholipids in seven tissues of cod (Gadus morhua), determined by a combined HPLC/GLC Meth. J. Chromatogr. 565: 119–129.

    Google Scholar 

  • Lie, #x00D8;., Hemre, G-I. and Lambertsen, G. 1992. Influence of dietary fatty acids on the glycerophospholipid composition in organs of cod (Gadus morhua). Lipids 27: 770–775.

    Google Scholar 

  • Lie, Ø., Sandvin, A. and WaagbØ, R. 1993. Influence of dietary fatty acids on the lipid composition of lipoproteins in farmed Atlantic salmon (Salmo salar). Fish Physiol. Biochem. 12: 249–260.

    Google Scholar 

  • Mourente, G. and Odriozola, J.M. 1990. Effect of broodstock diets on lipid classes and their fatty acid compositions in eggs of gilthead seabream (Sparus aurata L.). Fish Physiol. Biochem. 8: 93–101.

    Google Scholar 

  • Mourente, G. and Tocher, D.R. 1993. The effects of weaning on to a dry pellet diet on brain lipid and fatty acid compositions in post-larval gilthead seabream (Sparus aurata L.). Comp. Biochem. Physiol. 104A: 605–611.

    Google Scholar 

  • Napolitano, G.E., Ratmayake, W.M.N. and Ackman, R.G. 1988. Fatty acid component of larval Ostrea edulis L.: Importance of tryacilglycerols as a fatty acid reserve. Comp. Biochem. Physiol. 90B: 875–883.

    Google Scholar 

  • Olsen, R.E., Henderson, R.J. 1989. The rapid analysis of neutral and polar marine lipids using double-development HPTLC and scanning densitometry. J. Exp. Mar. Biol. Ecol. 129: 189–197.

    Google Scholar 

  • Olsen, R.E., Henderson, R.J. and Ringo, E. 1991. Lipids of Artic charr, Salvelinus alpinus (L.) I. Dietary induced changes in lipid class and fatty acid composition. Fish Physiol. Biochem. 9: 151–164.

    Google Scholar 

  • Owen, J.M., Adron, J.W., Middleton, C. and Cowey, C.B. 1975. Elongation and desaturation of dietary fatty acids in turbot (Scophthalmus maximus) and rainbow trout (Salmo gairdneri), Lipids 10: 528–531.

    Google Scholar 

  • Rodríguez, C., Pérez, J.A., Izquierdo, M.S., Mora, J., Lorenzo, A. and Fernández-Palacios, H. 1993. Essential fatty acid requeriments of larval gilthead seabream, Sparus aurata (L.). Aquacult. Fish. Manage. 24: 295–304.

    Google Scholar 

  • Rodríguez, C. 1994. Estudio de los requerimientos de ácidos grasos esenciales de la dorada europea (Sparus arurata L.) durante las dos primeras semanas de alimentación. Tesis doctoral, Universidad de La Laguna, Spain.

    Google Scholar 

  • Rodríguez, C., Pérez, J.A., Lorenzo, A., Izquierdo, M.S. and Cejas, J.R. 1994. n-3 HUFA requirement of larval gilthead seabream Sparus aurata when using high levels of eicosapentaenoic acid. Comp. Biochem. Physiol. 4: 693–698.

    Google Scholar 

  • RØnnestad, Y., Koven, W.M., Tandler; A., Harel, M. and Fyhn, H.J. 1994. Energy metabolism during development of eggs and larvae of gilthead seabream (Sparus aurata). Mar. Biol. 120: 187–196.

    Google Scholar 

  • Sargent, J., Henderson, R.J. and Tocher, D.R. 1989. The lipids. In Fish Nutrition. pp. 153–218. Edited by J.E. Halver. Academic Press, New York.

    Google Scholar 

  • Sargent, J.R. 1995. Origin and functions of eggs lipids: nutritional implications. In Broodstock Management and Egg and Larval Quality. pp. 353–372. Edited by N. R. Bromage and R. J. Roberts. Blackwell Science, London.

    Google Scholar 

  • Takeuchi, T. and Watanabe, T. 1977. Effect of eicosapentaenoic acid and docosahexaenoic acid in pollack liver oil on growth and fatty acid composition of rainbow trout. Bull. Jap. Soc. Sci. Fish. 43: 947–953.

    Google Scholar 

  • Takeuchi, T., Watanabe, T. and Nose, T. 1979. Requirement of essential fatty acid of chum salmon (Oncorhynchus keta) in freshwater environment. Bull. Jap. Soc. Sci. Fish. 45: 1319–1323.

    Google Scholar 

  • Takeuchi, T. and Watanabe, T. 1982. The effects of starvation and environmental temperature on proximate and fatty acid compositions of carp and rainbow trout. Bull. Jap. Soc. Sci. Fish. 48: 1307–1316

    Google Scholar 

  • Takeuchi, T., Toyota, M., Satoh, S. and Watanabe, T. 1990. Requirement of juvenile red seabream Pagrus major for eicosapentaenoic and docosahexaenoic acids. Nippon Suisan Gakkaishi 56: 1263–1269.

    Google Scholar 

  • Tandler, A., Har'el, M., Wilks, M., Levinson, A., Brickell, L., Christie, S., Avital, E. and Barr, Y. 1989. Effect of environmental temperature on survival, growth and population structure in the mass rearing of the gilthead seabream, Sparus aurata. Aquaculture 78: 277–284.

    Google Scholar 

  • Tocher, D.R., Fraser, A.J., Sargent, J.R. and Gamble, J.C. 1985a. Fatty acid composition of phospholipids and neutral lipids during embryonic and early larval development in Atlantic herring (Clupea harengus L). Lipids 20: 69–74.

    Google Scholar 

  • Tocher, D.R., Fraser, A.J., Sargent, J.R. and Gamble, J.C. 1985b. Lipid class composition during embryonic and early larval development in Atlantic herring (Clupea harengus L.). Lipids. 20: 84–89.

    Google Scholar 

  • Tocher, D.R., Mourente, G. and Sargent, J.R. 1992. Metabolism of [1-14C docosahexanoate (22:6n-3), [1-14C eicosapentanoate (20:5n-3) and [1-14C linolenate (18:3n-3) in brain cells from juvenile turbot, Scophthalmus maximus. Lipids 27: 494–499.

    Google Scholar 

  • Tocher, D.R. 1993. Elongation predominates over desaturation in the metabolism of 18:3n-3 and 20:5n-3 in turbot (Scophthalmus maximus) brain astroglial cells in primary culture. Lipids 28: 267–272.

    Google Scholar 

  • Ulvund, K.A. and Grahl-Nielsen, O. 1988. Fatty acid composition in eggs of Atlantic cod (Gadus morhua). Can. J. Fish. Aquat. Sci. 45: 898–901.

    Google Scholar 

  • Watanabe, T., Takeuchi, T. and Shimma, Y. 1978. Influence of SCP feed on lipid contents and fatty acid composition of rainbow trout. Bull. Freshw. Fish. Res. Lab. 28: 37–46.

    Google Scholar 

  • Watanabe, T., Takeuchi, T., Satoh, M. and Nishimura, K. 1984. Effect of low protein-high calory or essential fatty acid deficiency diet on reproduction of rainbow trout. Nippon Suisan Gakkaishi 50: 1207–1215.

    Google Scholar 

  • Watanabe, T., Lee, M., Mizutani, J., Yamada, T., Satoh, S., Takeuchi, T., Yoshida, N., Kitada, T. and Arakawa, T. 1991. Effective components in cuttlefish meal and raw krill for improvement of quality of red seabream (Pagrus major) eggs. Nippon Suisan Gakkaishi 57: 681–694.

    Google Scholar 

  • Watanabe, T. and Kiron, V. 1994. Broodstock management and nutritional approaches for quality offsprings in the red seabream. In: Proceedings, Broodstock Management and Egg and Larvae Quality. Stirling University 23–27 June 1992.

  • Zar, J.H. 1984. Biostadistical Analysis. 3rd edn., Prentice-Hall, Upper Saddle River, NI.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. N.E.R.C. Unit of Aquatic Biochemistry, Department of Biological and Molecular Sciences, University of Stirling, Stirling, FK9 4LA, Scotland, U.K

    C. Rodríguez

  2. Centro Oceanográfico de Canarias, Instituto Español de Oceanografía, Crta. San Andrés s/n, 38120, Sta. Cruz de Tenerife, Spain

    J.R. Cejas & M. Samper

  3. Departamento de Biología Animal, Facultad de Biología, Universidad de La Laguna, 38271, Tenerife, Spain

    M.V. Martín, P. Badía & A. Lorenzo

Authors
  1. C. Rodríguez
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J.R. Cejas
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M.V. Martín
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. P. Badía
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. M. Samper
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. A. Lorenzo
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rodríguez, C., Cejas, J., Martín, M. et al. Influence of n-3 highly unsaturated fatty acid deficiency on the lipid composition of broodstock gilthead seabream (Sparus aurata L.) and on egg quality. Fish Physiology and Biochemistry 18, 177–187 (1998). https://doi.org/10.1023/A:1007750218840

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1007750218840

Search

Navigation