Abstract
In spring 1992, 2-year-old bare-rooted seedlings of Sitka spruce (Picea sitchensis (Bong.) Carr.) and Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) were exposed for 0, 0.5, 1.0, 1.5, or 3 h in a controlled environment room at 20°C. Plants were desiccated in batches during February and March; 100 seedlings of each species were planted in 5 20-plant plots at each of 6 planting sites using the normal planting times and methods of each area. The effect of exposure was assessed within 24 h of desiccation on a subsample of plants by measuring fine root moisture content and electrolyte leakage and, in a limited number of cases, needle water potential. These measures were related to survival and growth after the first and second growing seasons.
There were significant interactions between the effect of desiccation and site. The measurements following desiccation were significantly related to survival and growth on some but not all sites; in general, the effect of condition was more pronounced on sites with low spring rainfall than on sites with greater than 100 mm rainfall per month during the spring of planting. Root electrolyte leakage was significantly related to performance in slightly more cases than needle water potential or root moisture content.
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Balneaves, J. and Menzies, M. 1988. Lifting and handling procedures at Edenvale Nursery – effects on survival and growth of 1/0 Pinus radiataseedlings. New Zealand Journal of Forestry Science 18: 132–134.
Bigras, F. J., and D'Aoust, A. L. 1992. Hardening and dehardening of shoots and roots of containerised black spruce and white spruce seedlings under short and long days. Can. J. For. Res. 22: 388–396.
Burdett, A. N. 1990. Physiological process in plantation establishment and the development of specifications for forest planting stock. Can. J. For. Res. 20: 415–427.
Burr, K. E., Tinus, R.W., Walner, S. J. and King, R. M. 1990. Comparison of 3 cold hardiness tests for conifers. Tree Physiology 6: 351–369.
Cleary, B. D. and Zaerr, J. B. 1980. Pressure chamber techniques for monitoring and evaluating seedling water status. N. Z. J. For. Sci. 10: 133–141.
Coleman, M. D., Hinckley, T. M., McNaughton, G. and Smit, B. A. 1992. Root cold hardiness and native distribution of subalpine conifers. Can. J. For. Res. 22: 932–938.
Colombo, S. J. 1994. Timing of cold temperature exposure affects root and shoot frost hardiness of Picea marianacontainer seedlings. Scand. J. For. Res. 9: 52–59.
Coutts, M. P. 1980. Control of water loss by actively growing Sitka spruce seedlings after transplanting. J. Exp. Bot. 125: 1587–1597.
Coutts, M. P. 1981a. Effects of root or shoot exposure before planting on the water relations, growth, and survival of Sitka spruce. Can. J. For. Res. 11: 703–709.
Coutts, M. P. 1981b. Leaf water potential and control of water loss in droughted Sitka spruce seedlings. J. Exp. Bot. 32: 1193–1201.
Deans, J. D., Lundeberg, C., Tabbush, P. M., Cannell, M. G. R., Sheppard, L. J. and Murray, M. D. 1990. The influence of desiccation, rough handling and cold storage on the quality and establishment of Sitka spruce planting stock. Forestry 63: 129–141.
Dlugokecka, E. and Kacperska-Palacz, A. 1978. Re-examination of electrical conductivity method for estimation of drought injury. Biol. Plant (Prague) 20: 262–267.
Feret, P. P., Kreh, R. E. and Mulligan, C. 1985. Effects of air drying on survival, height, and root growth potential of loblolly pine seedlings. Southern Journal of Applied Forestry 9: 125–128.
Green, L. M. and Warrington, I. J. 1978. Assessment of frost damage in radiata pine seedlings using the diffusate conductivity technique. New Zealand Journal of Forest Science 8: 344–350.
Hallam, P. H. and Tibbits, W. N. 1988. Determination of frost hardiness of Eucalyptususing the electrical conductivity of diffusate in conjunction with a freezing chamber. Can. J. For. Res. 18: 595–600.
Heinrich, J. C. 1977. Protection of planting stock during transplanting. Informations Forest 4: 159–166.
Huuri, O. 1972. The effect of unusual planting techniques on initial development of Scots pine and Norway spruce. Communicationes Instituti Forestalis Fenniae 75: 6, 99 pp.
Insley, H. 1979. Damage to Broadleaved Seedlings by Desiccation. Arboriculture Research Note. Department of the Environment, No 8, 4 pp.
Insley, H. and Buckley, G. P. 1985. The influence of desiccation and root pruning on the survival and growth of broadleaved seedlings. Journal of Horticultural Science 60: 377–387.
Lassoie, J. P. and Salo, D. J. 1981. Physiological response of large Douglas-fir to natural and induced soil water deficits. Can. J. For. Res. 11: 139–144.
Lassoie, J. P., Scott, D.R. M. and Fritschen, L. J. 1977. Stem sap velocity studies of transpiration in Douglas-fir using the heat pulse technique. For. Sci. 23: 377–390.
Martin, U., Pallardy, S. G. and Bahari, Z. A. 1987. Dehydration tolerance of leaf tissue of 6 woody angiosperm species. Physiol. Plant 69: 182–186.
McKay, H. M. 1992. Electrolyte leakage from fine roots of conifer seedlings: a rapid index of plant vitality following cold storage. Can. J. For. Res. 22: 1371–1377.
McKay, H.M. 1994. The Quality of Sitka Spruce at the Time of Planting. Research Information Note 243. Forestry Commission, UK. 4 pp.
McKay, H. M., Gardiner, B. A., Mason, W. L., Nelson, D. G. and Hollingsworth, M.K. 1993. The gravitational forces generated by dropping plants and the response of Sitka spruce seedlings to dropping. Can. J. For. Res. 23: 2443–2451.
McKay, H. M. and Mason, W. L. 1991. Physiological indicators of tolerance to cold storage in Sitka spruce and Douglas-fir seedlings. Can. J. For. Res. 21: 890–901.
Mullin, R. E. 1963. Planting check in Sitka spruce. For. Chron. 39: 252–259.
Murakami, P., Chen, T. H. H. and Fuchigami, L. H. 1990. Desiccation tolerance of deciduous plants during postharvest handling. Journal of Environmental Horticulture 8: 22–25.
Murray, M. B., Cape, J. N. and Fowler, D. 1989. Quantification of frost damage in plant tissues by rates of electrolyte leakage. New Phytologist 113: 307–311.
Palta, J. P., Levitt, J. and Stadelmann, E. J. 1977a. Freezing injury in onion bulb cells. I. Evaluation of the conductivity method and analysis of ion and sugar efflux from injured cells. Plant Physiol. 60: 393–397.
Palta, J. P., Levitt, J. and Stadelmann, E. J. 1977b. Freezing injury in onion bulb cells. II. Post-thawing injury and recovery. Plant Physiol. 60: 398–401.
Philipson, J. J. 1983. The role of gibberellin A4/7, heat and drought in the induction of flowering in Sitka spruce. J. Exp. Bot. 34: 291–302.
Philipson, J. J. 1988. Root growth in Sitka spruce and Douglas-fir transplants: dependence on the shoot and stored carbohydrate. Tree Physiol. 4: 101–108.
Rietveld, R. J. 1989. Transplanting stress in bareroot conifer seedlings: its development and progression to establishment. North. J. Appl. For. 6: 99–107.
Ritchie, G. A. 1986. Relationship among bud dormancy status, cold hardiness, and stress resistance in 2+0 Douglas-fir. New Forests 1: 29–42.
Sharpe, A. L., Howes, R. E. J. and Mason, W. L. 1988. Precision Sown and Undercut Conifer Planting Stock. II. Nursery regimes. Forestry Commission Research Information Note (U.K.) 132.
Sharpe, A. L. and Mason, W. L. 1992. Some methods of cold storage can seriously affect root growth potential and root moisture content and subsequent forest performance of Sitka spruce and Douglas-fir transplants. Forestry 65: 463–472.
Simpson, D. G., Thompson, C. F. and Sutherland, C. D. 1994. Field performance potential of interior spruce seedings: effects of stress treatments and prediction by root growth potential and needle conductance. Can. J. For. Res. 24: 576–586.
Tabbush, P. M. 1987. Effect of desiccation on water status and forest performance of barerooted Sitka spruce and Douglas-fir transplants. Forestry 60: 31–43.
van den Driessche, R. 1987. Importance of current photosynthate to new root growth in planted conifer seedlings. Can. J. For. Res. 17: 776–782.
Wilner, J. 1960. Relative and absolute electrolyte conductance tests for frost hardiness of apple varieties. Can. J. Plant. Sci. 40: 630–637.
Zabielski, S. 1969. The effect of various factors on the success of planting out in Poplars. Bull. Serv. Cult. Etud. Peuplier et Saule 1969, 33–53.
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McKay, H., White, M. Fine root electrolyte leakage and moisture content: indices of Sitka spruce and Douglas-fir seedling performance after desiccation. New Forests 13, 139–162 (1997). https://doi.org/10.1023/A:1006571805960
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DOI: https://doi.org/10.1023/A:1006571805960