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Degradation of the Photosystem II D1 and D2 proteins in different strains of the cyanobacterium Synechocystis PCC 6803 varying with respect to the type and level of psbA transcript

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Abstract

The turnover of the D1 and D2 proteins of Photosystem II (PSII) has been investigated by pulse-chase radiolabeling in several strains of the cyanobacterium Synechocystis PCC 6803 containing different types and levels of the psbA transcript. Strains lacking psbA1 and psbA3 gene and containing high levels of the psbA2 transcript showed the selective synthesis of D1 whose degradation could be slowed down by the protein synthesis inhibitor lincomycin. In contrast, in strains containing just the psbA3 gene, the intensity of the D1 protein labeling was lower and labeling of the D2 and CP43 proteins was stimulated in comparison to the psbA2-containing strains. In addition, the rate and selectivity of the D1 degradation and its dependence on the presence of lincomycin was proportional to the level of the psbA3 transcript in the particular strain. Consequently, there was parallel, lincomycin-independent and slowed-down breakdown of the D1 and D2 proteins in strains with the lowest level of psbA3 transcript. These results are discussed in terms of a model in which the rate of D1 and D2 degradation in cyanobacteria is affected not only by the rate of PSII photodamage, but also by the availability of newly synthesized D1 protein. Moreover, the comparison of the non-oxygen-evolving D1 mutants D170A** and Y161F*** differing by the presence of tyrosine Z has indicated a minor role of the oxidized form of this secondary PSII electron donor in the donor side mechanism of D1 and D2 protein breakdown.

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Authors and Affiliations

  1. Laboratory of Photosynthesis, Institute of Microbiology, Academy of Sciences, Opatovickýmlýn, 37981, Třeboň, Czech Republic

    Josef Komenda

  2. Department of Biochemistry, Imperial College of Science, Technology & Medicine, London, SW7 2AY, UK

    Hanadi A.G. Hassan, James Barber & Peter J. Nixon

  3. Central Research and Development Department, E.I. Du Pont de Nemours & Company, Experimental Station, P.O. Box 80 173, Wilmington, DE, 19880-0173, USA

    Bruce A. Diner

  4. Department of Biochemistry, University of California at Riverside, Riverside, CA, 92521-0129, USA

    Richard J. Debus

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  1. Josef Komenda
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  2. Hanadi A.G. Hassan
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  4. Richard J. Debus
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  5. James Barber
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  6. Peter J. Nixon
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Komenda, J., Hassan, H.A., Diner, B.A. et al. Degradation of the Photosystem II D1 and D2 proteins in different strains of the cyanobacterium Synechocystis PCC 6803 varying with respect to the type and level of psbA transcript. Plant Mol Biol 42, 635–645 (2000). https://doi.org/10.1023/A:1006305308196

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