Abstract
A hyper-thermostable, alkaline lipase from a newly-isolated, mesophilic Pseudomonas sp. was optimal at pH 11 and at 90 °C. It had a half-life of more than 13 h at 90 °C. It was activated by 30% when heated at 90 °C for 2 h. The enzyme had a greater affinity for mustard oil (K m=40 mg ml−1) than for olive oil (K m=140 mg ml−1).
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Rathi, P., Bradoo, S., Saxena, R. et al. A hyper-thermostable, alkaline lipase from Pseudomonas sp. with the property of thermal activation. Biotechnology Letters 22, 495–498 (2000). https://doi.org/10.1023/A:1005604617440
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DOI: https://doi.org/10.1023/A:1005604617440