Abstract
Nocardia tartaricans converted sodium cis-epoxysuccinate to L-tartrate. The highest cis-epoxysuccinate hydrolase activity (37.7 U mg−1) was obtained with 0.02% (w/v) sodium deoxycholate, but this inactivated the cells. Immobilized N. tartaricans in pectate gel showed higher enzyme activity (51 U mg −1) compare to the free cells (8.9 U mg −1). After 450 days, the immobilized cells still possessed 0.65 U mg −1, i.e. 30% of the initial enzyme activity.
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Gemeiner P, Nahálka J, Vikartovská A, Nahálková J, Tomáška M, Šturdík E, Markovič O, Malovíková A, Zatková I, Ilavský M (1996) In: Wiffels RH, Buitelaar RM, Bucke C, Tramper J, eds. Immobilized Cells: Basics and Applications. Amsterdam: Elsevier Science B.V., pp. 76–83.
Kurillová L', Gemeiner P, Ilavsky M, Štefuca V, Polakovič M, Welwardová A, Tóth D (1992) Calcium pectate beads for cell entrapment. 4. Properties of stabilized and hardened calcium pectate gel beads with and without cells. Biotechnol. Appl. Biochem. 16: 236–251.
Miková H, Rosenberg M, Liptaj T (1998) Influence of molybdate and tungstate ions on activity of cis-epoxysuccinate hydrolase in Nocardia tartaricans. Biotechnol. Lett. 20: 833–835.
Miura Y, Yutani K, Takesue H (1977) Microbiological process for preparing of L-tartaric acid in presence of surfactans. U.S. Pat. 4 017 362.
Norton S, Vuillemard JCh (1994) Food bioconversions and metabolite production using immobilized cell technology. Crit. Rev. Biotechnol. 14: 193–224.
Polonský J, Repášová L', Pekarovičová A, Košík M (1985) Isotachophoretic analysis of saccharidic acids. J. Chromatogr. 320: 111–118.
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Rosenberg, M., Miková, H. & Krištofíková, L. Production of L-tartaric acid by immobilized bacterial cells Nocardia tartaricans . Biotechnology Letters 21, 491–495 (1999). https://doi.org/10.1023/A:1005592104426
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DOI: https://doi.org/10.1023/A:1005592104426