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Influence of molybdate and tungstate ions on activity of cis-epoxysuccinate hydrolase in Nocardia tartaricans

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Abstract

L-Tartaric acid was produced by transformation of cis-epoxysuccinic acid using Nocardia tartaricans ATCC 31191 in a molar yield of 100%. cis-Epoxysuccinic acid was prepared by epoxidation of maleic acid in the presence of molybdate or tungstate ions. The effect of these ions on the subsequent activity of the cis-epoxysuccinate hydrolase was determined in both intact cells and cell-free extracts of Nocardia tartaricans. Based on kinetic studies, the tungstate ions behaved as a strong inhibitor while the molybdate ions as a stimulator of the enzyme. © Rapid Science Ltd. 1998

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Authors and Affiliations

  1. Department of Biochemical Technology, Faculty of Chemical Technology, Slovak Technical University, Radlinského, Bratislava, Slovakia

    H. Miková, M. Rosenberg & L'. Kristofíková

  2. Laboratory of NMR Spectroscopy, Faculty of Chemical Technology, Slovak Technical University, Radlinského, Bratislava, Slovakia

    T. Liptaj

Authors
  1. H. Miková
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  2. M. Rosenberg
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  3. L'. Kristofíková
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  4. T. Liptaj
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Miková, H., Rosenberg, M., Kristofíková, L. et al. Influence of molybdate and tungstate ions on activity of cis-epoxysuccinate hydrolase in Nocardia tartaricans. Biotechnology Letters 20, 833–835 (1998). https://doi.org/10.1023/A:1005303307226

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  • DOI: https://doi.org/10.1023/A:1005303307226

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